Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10500 and PS417_10505 are separated by 0 nucleotides PS417_10505 and PS417_10510 are separated by 149 nucleotides PS417_10510 and PS417_10515 are separated by 187 nucleotides
PS417_10500: PS417_10500 - lipoprotein, at 2,241,155 to 2,242,240
_10500
PS417_10505: PS417_10505 - hypothetical protein, at 2,242,241 to 2,243,089
_10505
PS417_10510: PS417_10510 - esterase, at 2,243,239 to 2,243,655
_10510
PS417_10515: PS417_10515 - acetyl-CoA acetyltransferase, at 2,243,843 to 2,245,024
_10515
Position (kb)
2243
2244 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2242.380 kb on + strand, within PS417_10505 at 2242.380 kb on + strand, within PS417_10505 at 2242.381 kb on - strand, within PS417_10505 at 2242.381 kb on - strand, within PS417_10505 at 2243.121 kb on + strand at 2243.204 kb on + strand at 2243.204 kb on + strand at 2243.204 kb on + strand at 2243.204 kb on + strand at 2243.205 kb on - strand at 2243.223 kb on + strand at 2243.256 kb on - strand at 2243.727 kb on + strand at 2243.727 kb on + strand at 2243.728 kb on - strand at 2243.827 kb on + strand at 2243.827 kb on + strand at 2243.828 kb on - strand at 2243.828 kb on - strand at 2244.174 kb on - strand, within PS417_10515 at 2244.174 kb on - strand, within PS417_10515
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x remove 2,242,380 + PS417_10505 0.16 -0.6 2,242,380 + PS417_10505 0.16 +0.2 2,242,381 - PS417_10505 0.16 -0.2 2,242,381 - PS417_10505 0.16 +0.7 2,243,121 + +3.5 2,243,204 + +1.1 2,243,204 + +2.1 2,243,204 + -2.1 2,243,204 + -0.2 2,243,205 - -0.1 2,243,223 + +0.5 2,243,256 - +1.2 2,243,727 + +0.3 2,243,727 + +2.7 2,243,728 - -0.5 2,243,827 + -0.7 2,243,827 + -1.1 2,243,828 - -0.4 2,243,828 - +0.0 2,244,174 - PS417_10515 0.28 -0.1 2,244,174 - PS417_10515 0.28 +0.4
Or see this region's nucleotide sequence