Experiment: CTR
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt amyA and yedD are separated by 33 nucleotides yedD and yedE are separated by 186 nucleotides
b1927: amyA - cytoplasmic alpha-amylase (NCBI), at 2,004,180 to 2,005,667
amyA
b1928: yedD - hypothetical protein (NCBI), at 2,005,701 to 2,006,114
yedD
b1929: yedE - predicted inner membrane protein (NCBI), at 2,006,301 to 2,007,506
yedE
Position (kb)
2005
2006
2007 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2004.739 kb on - strand, within amyA at 2004.809 kb on + strand, within amyA at 2004.861 kb on - strand, within amyA at 2004.942 kb on - strand, within amyA at 2005.017 kb on - strand, within amyA at 2005.185 kb on + strand, within amyA at 2005.185 kb on + strand, within amyA at 2005.308 kb on + strand, within amyA at 2005.308 kb on + strand, within amyA at 2005.310 kb on + strand, within amyA at 2005.410 kb on - strand, within amyA at 2005.410 kb on - strand, within amyA at 2005.443 kb on - strand, within amyA at 2005.591 kb on - strand at 2005.633 kb on + strand at 2005.633 kb on + strand at 2005.659 kb on - strand at 2005.659 kb on - strand at 2005.729 kb on + strand at 2005.787 kb on - strand, within yedD at 2005.788 kb on + strand, within yedD at 2005.801 kb on - strand, within yedD at 2005.801 kb on - strand, within yedD at 2005.809 kb on - strand, within yedD at 2005.827 kb on - strand, within yedD at 2005.953 kb on + strand, within yedD at 2005.969 kb on - strand, within yedD at 2006.256 kb on - strand at 2006.284 kb on + strand at 2006.393 kb on - strand at 2006.393 kb on - strand at 2006.417 kb on + strand at 2006.417 kb on + strand at 2006.457 kb on - strand, within yedE at 2006.508 kb on + strand, within yedE at 2006.605 kb on - strand, within yedE at 2006.732 kb on - strand, within yedE at 2006.767 kb on - strand, within yedE at 2006.772 kb on - strand, within yedE at 2006.951 kb on - strand, within yedE at 2006.989 kb on + strand, within yedE at 2007.062 kb on + strand, within yedE at 2007.062 kb on + strand, within yedE
Per-strain Table
Position Strand Gene LocusTag Fraction CTR remove 2,004,739 - amyA b1927 0.38 +1.7 2,004,809 + amyA b1927 0.42 -1.6 2,004,861 - amyA b1927 0.46 +1.5 2,004,942 - amyA b1927 0.51 +0.4 2,005,017 - amyA b1927 0.56 +1.7 2,005,185 + amyA b1927 0.68 -1.5 2,005,185 + amyA b1927 0.68 +1.1 2,005,308 + amyA b1927 0.76 -1.2 2,005,308 + amyA b1927 0.76 +0.4 2,005,310 + amyA b1927 0.76 -0.2 2,005,410 - amyA b1927 0.83 -0.3 2,005,410 - amyA b1927 0.83 +1.6 2,005,443 - amyA b1927 0.85 -0.9 2,005,591 - +0.7 2,005,633 + -1.0 2,005,633 + -1.5 2,005,659 - +2.2 2,005,659 - +1.0 2,005,729 + -1.1 2,005,787 - yedD b1928 0.21 -3.6 2,005,788 + yedD b1928 0.21 +0.6 2,005,801 - yedD b1928 0.24 +0.1 2,005,801 - yedD b1928 0.24 +1.3 2,005,809 - yedD b1928 0.26 -0.2 2,005,827 - yedD b1928 0.30 -0.1 2,005,953 + yedD b1928 0.61 +3.2 2,005,969 - yedD b1928 0.65 +1.3 2,006,256 - -1.4 2,006,284 + +0.2 2,006,393 - -0.9 2,006,393 - +0.0 2,006,417 + -0.3 2,006,417 + +0.1 2,006,457 - yedE b1929 0.13 -0.1 2,006,508 + yedE b1929 0.17 -0.5 2,006,605 - yedE b1929 0.25 -0.2 2,006,732 - yedE b1929 0.36 -1.3 2,006,767 - yedE b1929 0.39 +0.4 2,006,772 - yedE b1929 0.39 -2.3 2,006,951 - yedE b1929 0.54 +0.2 2,006,989 + yedE b1929 0.57 -0.7 2,007,062 + yedE b1929 0.63 +1.1 2,007,062 + yedE b1929 0.63 +1.4
Or see this region's nucleotide sequence