Experiment: PoolT9D24
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrB and pyrL are separated by 3 nucleotides pyrL and yjgH are separated by 280 nucleotides yjgH and yjgI are separated by 130 nucleotides yjgI and yjgJ are separated by 70 nucleotides
b4245: pyrB - aspartate carbamoyltransferase catalytic subunit (NCBI), at 4,469,483 to 4,470,418
pyrB
b4246: pyrL - pyrBI operon leader peptide (NCBI), at 4,470,422 to 4,470,556
pyrL
b4248: yjgH - predicted mRNA endoribonuclease (NCBI), at 4,470,837 to 4,471,232
yjgH
b4249: yjgI - predicted oxidoreductase with NAD(P)-binding Rossmann-fold domain (NCBI), at 4,471,363 to 4,472,076
yjgI
b4251: yjgJ - orf, hypothetical protein (VIMSS), at 4,472,147 to 4,472,740
yjgJ
Position (kb)
4470
4471
4472 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 4469.937 kb on - strand, within pyrB at 4469.945 kb on + strand, within pyrB at 4469.952 kb on + strand, within pyrB at 4469.966 kb on + strand, within pyrB at 4470.043 kb on + strand, within pyrB at 4470.104 kb on + strand, within pyrB at 4470.104 kb on + strand, within pyrB at 4470.106 kb on + strand, within pyrB at 4470.200 kb on + strand, within pyrB at 4470.224 kb on + strand, within pyrB at 4470.257 kb on + strand, within pyrB at 4470.359 kb on - strand at 4470.427 kb on - strand at 4470.427 kb on - strand at 4470.466 kb on + strand, within pyrL at 4470.466 kb on + strand, within pyrL at 4470.482 kb on - strand, within pyrL at 4470.718 kb on + strand at 4470.764 kb on + strand at 4470.783 kb on - strand at 4470.783 kb on - strand at 4470.796 kb on + strand at 4470.796 kb on + strand at 4470.808 kb on + strand at 4470.830 kb on + strand at 4470.830 kb on - strand at 4470.830 kb on - strand at 4470.840 kb on + strand at 4470.842 kb on + strand at 4470.842 kb on + strand at 4470.842 kb on - strand at 4470.898 kb on - strand, within yjgH at 4470.899 kb on - strand, within yjgH at 4470.988 kb on + strand, within yjgH at 4470.996 kb on - strand, within yjgH at 4470.996 kb on - strand, within yjgH at 4470.996 kb on - strand, within yjgH at 4471.004 kb on - strand, within yjgH at 4471.009 kb on - strand, within yjgH at 4471.012 kb on - strand, within yjgH at 4471.097 kb on + strand, within yjgH at 4471.176 kb on - strand, within yjgH at 4471.354 kb on - strand at 4471.362 kb on + strand at 4471.367 kb on + strand at 4471.378 kb on - strand at 4471.388 kb on - strand at 4471.388 kb on - strand at 4471.667 kb on + strand, within yjgI at 4471.680 kb on - strand, within yjgI at 4471.719 kb on + strand, within yjgI at 4471.719 kb on + strand, within yjgI at 4471.851 kb on - strand, within yjgI at 4471.851 kb on - strand, within yjgI at 4471.862 kb on - strand, within yjgI at 4471.869 kb on - strand, within yjgI at 4471.869 kb on - strand, within yjgI at 4471.909 kb on - strand, within yjgI at 4471.909 kb on - strand, within yjgI at 4471.932 kb on - strand, within yjgI at 4471.982 kb on - strand, within yjgI at 4472.190 kb on - strand at 4472.196 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PoolT9D24 remove 4,469,937 - pyrB b4245 0.49 +0.1 4,469,945 + pyrB b4245 0.49 +0.6 4,469,952 + pyrB b4245 0.50 -1.3 4,469,966 + pyrB b4245 0.52 +0.6 4,470,043 + pyrB b4245 0.60 -1.4 4,470,104 + pyrB b4245 0.66 +0.2 4,470,104 + pyrB b4245 0.66 +0.6 4,470,106 + pyrB b4245 0.67 +0.4 4,470,200 + pyrB b4245 0.77 -0.1 4,470,224 + pyrB b4245 0.79 -0.7 4,470,257 + pyrB b4245 0.83 -0.3 4,470,359 - -0.4 4,470,427 - -1.1 4,470,427 - +0.4 4,470,466 + pyrL b4246 0.33 -0.3 4,470,466 + pyrL b4246 0.33 +0.5 4,470,482 - pyrL b4246 0.44 -1.1 4,470,718 + +1.0 4,470,764 + +0.8 4,470,783 - +0.1 4,470,783 - -1.9 4,470,796 + -2.0 4,470,796 + -0.4 4,470,808 + -0.8 4,470,830 + +0.5 4,470,830 - +0.5 4,470,830 - +0.8 4,470,840 + +1.0 4,470,842 + +0.8 4,470,842 + -2.3 4,470,842 - -0.5 4,470,898 - yjgH b4248 0.15 -0.3 4,470,899 - yjgH b4248 0.16 -0.5 4,470,988 + yjgH b4248 0.38 -0.8 4,470,996 - yjgH b4248 0.40 -0.6 4,470,996 - yjgH b4248 0.40 -0.6 4,470,996 - yjgH b4248 0.40 +0.4 4,471,004 - yjgH b4248 0.42 -0.0 4,471,009 - yjgH b4248 0.43 -0.1 4,471,012 - yjgH b4248 0.44 -1.4 4,471,097 + yjgH b4248 0.66 -1.2 4,471,176 - yjgH b4248 0.86 -0.8 4,471,354 - -0.8 4,471,362 + +3.9 4,471,367 + -0.5 4,471,378 - -0.6 4,471,388 - -1.0 4,471,388 - -1.3 4,471,667 + yjgI b4249 0.43 -0.4 4,471,680 - yjgI b4249 0.44 -0.7 4,471,719 + yjgI b4249 0.50 -1.1 4,471,719 + yjgI b4249 0.50 +1.0 4,471,851 - yjgI b4249 0.68 -1.4 4,471,851 - yjgI b4249 0.68 -1.2 4,471,862 - yjgI b4249 0.70 -0.3 4,471,869 - yjgI b4249 0.71 -0.6 4,471,869 - yjgI b4249 0.71 -1.2 4,471,909 - yjgI b4249 0.76 -1.1 4,471,909 - yjgI b4249 0.76 +0.5 4,471,932 - yjgI b4249 0.80 -0.0 4,471,982 - yjgI b4249 0.87 +0.1 4,472,190 - +0.3 4,472,196 + -0.1
Or see this region's nucleotide sequence