Strain Fitness in Escherichia coli BW25113 around b0260

Experiment: PoolT9D23

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntinsH-1 and ykfD are separated by 207 nucleotidesykfD and mmuM overlap by 14 nucleotides b0259: insH-1 - CP4-6 prophage; IS5 transposase and trans-activator (NCBI), at 273,325 to 274,341 insH-1 b0260: ykfD - putative amino acid/amine transport protein (VIMSS), at 274,549 to 275,952 ykfD b0261: mmuM - homocysteine methyltransferase (NCBI), at 275,939 to 276,871 mmuM Position (kb) 274 275 276Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 274.371 kb on + strandat 274.376 kb on - strandat 274.376 kb on - strandat 274.428 kb on + strandat 274.461 kb on + strandat 274.497 kb on + strandat 274.712 kb on + strand, within ykfDat 274.725 kb on - strand, within ykfDat 274.725 kb on - strand, within ykfDat 274.781 kb on - strand, within ykfDat 274.781 kb on - strand, within ykfDat 274.790 kb on + strand, within ykfDat 274.791 kb on - strand, within ykfDat 274.851 kb on + strand, within ykfDat 274.855 kb on - strand, within ykfDat 274.908 kb on - strand, within ykfDat 274.913 kb on - strand, within ykfDat 274.932 kb on + strand, within ykfDat 274.950 kb on - strand, within ykfDat 274.950 kb on - strand, within ykfDat 274.955 kb on - strand, within ykfDat 275.190 kb on - strand, within ykfDat 275.516 kb on - strand, within ykfDat 275.585 kb on - strand, within ykfDat 275.627 kb on - strand, within ykfDat 275.883 kb on + strandat 275.883 kb on + strandat 275.976 kb on - strandat 275.986 kb on - strandat 275.988 kb on - strandat 275.988 kb on - strandat 276.000 kb on - strandat 276.059 kb on + strand, within mmuMat 276.073 kb on - strand, within mmuMat 276.073 kb on - strand, within mmuMat 276.333 kb on + strand, within mmuMat 276.346 kb on - strand, within mmuMat 276.346 kb on - strand, within mmuMat 276.358 kb on + strand, within mmuMat 276.415 kb on + strand, within mmuMat 276.580 kb on - strand, within mmuMat 276.621 kb on + strand, within mmuMat 276.621 kb on + strand, within mmuMat 276.669 kb on + strand, within mmuMat 276.677 kb on - strand, within mmuMat 276.682 kb on + strand, within mmuMat 276.685 kb on + strand, within mmuMat 276.882 kb on + strandat 276.890 kb on - strandat 276.890 kb on - strandat 276.890 kb on - strandat 276.890 kb on - strandat 276.931 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction PoolT9D23
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274,371 + +0.5
274,376 - +0.4
274,376 - +1.1
274,428 + -1.4
274,461 + -0.6
274,497 + +2.7
274,712 + ykfD b0260 0.12 +2.0
274,725 - ykfD b0260 0.13 -0.6
274,725 - ykfD b0260 0.13 -1.6
274,781 - ykfD b0260 0.17 -0.1
274,781 - ykfD b0260 0.17 -1.2
274,790 + ykfD b0260 0.17 -0.3
274,791 - ykfD b0260 0.17 +0.6
274,851 + ykfD b0260 0.22 -0.4
274,855 - ykfD b0260 0.22 -1.2
274,908 - ykfD b0260 0.26 -0.9
274,913 - ykfD b0260 0.26 -0.7
274,932 + ykfD b0260 0.27 -0.8
274,950 - ykfD b0260 0.29 -1.7
274,950 - ykfD b0260 0.29 +0.4
274,955 - ykfD b0260 0.29 -0.9
275,190 - ykfD b0260 0.46 -0.9
275,516 - ykfD b0260 0.69 -0.7
275,585 - ykfD b0260 0.74 -1.1
275,627 - ykfD b0260 0.77 -1.2
275,883 + +0.3
275,883 + -2.6
275,976 - -1.9
275,986 - -0.2
275,988 - -0.2
275,988 - +0.7
276,000 - -0.6
276,059 + mmuM b0261 0.13 +0.7
276,073 - mmuM b0261 0.14 -0.3
276,073 - mmuM b0261 0.14 +0.7
276,333 + mmuM b0261 0.42 -0.9
276,346 - mmuM b0261 0.44 -0.5
276,346 - mmuM b0261 0.44 -0.5
276,358 + mmuM b0261 0.45 -0.2
276,415 + mmuM b0261 0.51 -0.2
276,580 - mmuM b0261 0.69 -0.4
276,621 + mmuM b0261 0.73 +0.3
276,621 + mmuM b0261 0.73 -0.2
276,669 + mmuM b0261 0.78 -0.6
276,677 - mmuM b0261 0.79 -2.8
276,682 + mmuM b0261 0.80 -0.2
276,685 + mmuM b0261 0.80 -0.8
276,882 + -0.3
276,890 - -0.2
276,890 - -0.3
276,890 - +0.4
276,890 - -2.6
276,931 + +0.2

Or see this region's nucleotide sequence