Experiment: PoolT9D23
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt caiA and caiT are separated by 30 nucleotides caiT and fixA are separated by 471 nucleotides
b0039: caiA - crotonobetaine reductase subunit II, FAD-binding (NCBI), at 39,244 to 40,386
caiA
b0040: caiT - L-carnitine/gamma-butyrobetaine antiporter (NCBI), at 40,417 to 41,931
caiT
b0041: fixA - probable flavoprotein subunit, carnitine metabolism (VIMSS), at 42,403 to 43,173
fixA
Position (kb) 40
41
42 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 39.439 kb on - strand, within caiA at 39.472 kb on - strand, within caiA at 39.496 kb on - strand, within caiA at 39.589 kb on + strand, within caiA at 39.640 kb on - strand, within caiA at 39.640 kb on - strand, within caiA at 39.746 kb on - strand, within caiA at 39.800 kb on - strand, within caiA at 39.800 kb on - strand, within caiA at 39.849 kb on + strand, within caiA at 39.910 kb on - strand, within caiA at 39.982 kb on - strand, within caiA at 39.982 kb on - strand, within caiA at 40.143 kb on + strand, within caiA at 40.143 kb on + strand, within caiA at 40.179 kb on + strand, within caiA at 40.179 kb on + strand, within caiA at 40.179 kb on + strand, within caiA at 40.195 kb on + strand, within caiA at 40.203 kb on - strand, within caiA at 40.227 kb on - strand, within caiA at 40.227 kb on - strand, within caiA at 40.529 kb on - strand at 40.540 kb on + strand at 40.545 kb on + strand at 40.591 kb on + strand, within caiT at 40.646 kb on + strand, within caiT at 40.646 kb on + strand, within caiT at 40.646 kb on + strand, within caiT at 40.654 kb on - strand, within caiT at 40.654 kb on - strand, within caiT at 40.668 kb on + strand, within caiT at 40.668 kb on + strand, within caiT at 40.701 kb on - strand, within caiT at 40.766 kb on - strand, within caiT at 40.766 kb on - strand, within caiT at 40.774 kb on - strand, within caiT at 40.839 kb on - strand, within caiT at 40.850 kb on + strand, within caiT at 40.889 kb on - strand, within caiT at 40.902 kb on - strand, within caiT at 40.902 kb on - strand, within caiT at 40.902 kb on - strand, within caiT at 40.908 kb on + strand, within caiT at 40.912 kb on - strand, within caiT at 40.918 kb on - strand, within caiT at 40.947 kb on - strand, within caiT at 40.947 kb on - strand, within caiT at 40.947 kb on - strand, within caiT at 40.947 kb on - strand, within caiT at 40.972 kb on + strand, within caiT at 41.029 kb on - strand, within caiT at 41.029 kb on - strand, within caiT at 41.146 kb on + strand, within caiT at 41.158 kb on + strand, within caiT at 41.158 kb on + strand, within caiT at 41.179 kb on - strand, within caiT at 41.256 kb on - strand, within caiT at 41.282 kb on - strand, within caiT at 41.318 kb on - strand, within caiT at 41.329 kb on + strand, within caiT at 41.409 kb on + strand, within caiT at 41.411 kb on + strand, within caiT at 41.417 kb on - strand, within caiT at 41.417 kb on - strand, within caiT at 41.487 kb on - strand, within caiT at 41.574 kb on - strand, within caiT at 41.709 kb on - strand, within caiT at 41.709 kb on - strand, within caiT at 41.714 kb on + strand, within caiT at 41.716 kb on + strand, within caiT at 41.716 kb on + strand, within caiT at 41.723 kb on - strand, within caiT at 41.726 kb on + strand, within caiT at 41.726 kb on + strand, within caiT at 41.734 kb on - strand, within caiT at 41.734 kb on - strand, within caiT at 41.734 kb on - strand, within caiT at 41.734 kb on - strand, within caiT at 41.734 kb on - strand, within caiT at 41.734 kb on - strand, within caiT at 41.753 kb on + strand, within caiT at 41.753 kb on + strand, within caiT at 41.753 kb on + strand, within caiT at 41.753 kb on + strand, within caiT at 41.753 kb on + strand, within caiT at 41.753 kb on + strand, within caiT at 41.757 kb on - strand, within caiT at 41.757 kb on - strand, within caiT at 41.758 kb on + strand, within caiT at 41.759 kb on - strand, within caiT at 41.761 kb on + strand, within caiT at 41.761 kb on + strand, within caiT at 41.761 kb on - strand, within caiT at 41.761 kb on - strand, within caiT at 41.771 kb on + strand, within caiT at 41.771 kb on + strand, within caiT at 41.771 kb on + strand, within caiT at 41.771 kb on + strand, within caiT at 41.771 kb on + strand, within caiT at 41.774 kb on + strand, within caiT at 41.779 kb on + strand, within caiT at 41.783 kb on + strand at 41.786 kb on - strand at 41.791 kb on - strand at 41.791 kb on - strand at 41.794 kb on - strand at 41.795 kb on + strand at 41.797 kb on + strand at 41.803 kb on - strand at 41.830 kb on + strand at 41.830 kb on + strand at 41.830 kb on + strand at 41.838 kb on - strand at 41.838 kb on - strand at 41.844 kb on - strand at 41.846 kb on - strand at 41.849 kb on + strand at 41.850 kb on + strand at 41.938 kb on + strand at 42.028 kb on + strand at 42.028 kb on + strand at 42.028 kb on + strand at 42.040 kb on + strand at 42.041 kb on + strand at 42.041 kb on + strand at 42.041 kb on + strand at 42.041 kb on + strand at 42.043 kb on + strand at 42.048 kb on - strand at 42.049 kb on - strand at 42.059 kb on - strand at 42.070 kb on - strand at 42.070 kb on - strand at 42.070 kb on - strand at 42.070 kb on - strand at 42.097 kb on + strand at 42.098 kb on + strand at 42.098 kb on + strand at 42.430 kb on - strand at 42.442 kb on - strand at 42.446 kb on + strand at 42.447 kb on - strand at 42.452 kb on - strand at 42.452 kb on - strand at 42.456 kb on + strand at 42.464 kb on - strand at 42.464 kb on - strand at 42.466 kb on - strand at 42.466 kb on - strand at 42.466 kb on - strand at 42.466 kb on - strand at 42.466 kb on - strand at 42.470 kb on + strand at 42.478 kb on - strand at 42.478 kb on - strand at 42.478 kb on - strand at 42.485 kb on + strand, within fixA at 42.488 kb on - strand, within fixA at 42.491 kb on - strand, within fixA at 42.491 kb on - strand, within fixA at 42.534 kb on - strand, within fixA at 42.541 kb on + strand, within fixA at 42.550 kb on - strand, within fixA at 42.591 kb on - strand, within fixA at 42.601 kb on - strand, within fixA at 42.606 kb on - strand, within fixA at 42.682 kb on - strand, within fixA at 42.745 kb on + strand, within fixA at 42.873 kb on - strand, within fixA
Per-strain Table
Position Strand Gene LocusTag Fraction PoolT9D23 remove 39,439 - caiA b0039 0.17 +0.7 39,472 - caiA b0039 0.20 -0.4 39,496 - caiA b0039 0.22 -0.9 39,589 + caiA b0039 0.30 +0.6 39,640 - caiA b0039 0.35 -0.6 39,640 - caiA b0039 0.35 -1.1 39,746 - caiA b0039 0.44 +1.0 39,800 - caiA b0039 0.49 +2.1 39,800 - caiA b0039 0.49 -0.5 39,849 + caiA b0039 0.53 +0.0 39,910 - caiA b0039 0.58 +0.1 39,982 - caiA b0039 0.65 -2.9 39,982 - caiA b0039 0.65 -1.3 40,143 + caiA b0039 0.79 -1.3 40,143 + caiA b0039 0.79 -0.5 40,179 + caiA b0039 0.82 -0.1 40,179 + caiA b0039 0.82 +0.8 40,179 + caiA b0039 0.82 -0.2 40,195 + caiA b0039 0.83 +0.7 40,203 - caiA b0039 0.84 +0.7 40,227 - caiA b0039 0.86 -1.2 40,227 - caiA b0039 0.86 -0.5 40,529 - +0.9 40,540 + -2.2 40,545 + -0.1 40,591 + caiT b0040 0.11 -2.4 40,646 + caiT b0040 0.15 +1.3 40,646 + caiT b0040 0.15 -0.3 40,646 + caiT b0040 0.15 -0.2 40,654 - caiT b0040 0.16 -0.1 40,654 - caiT b0040 0.16 +1.2 40,668 + caiT b0040 0.17 -0.9 40,668 + caiT b0040 0.17 +0.2 40,701 - caiT b0040 0.19 +1.0 40,766 - caiT b0040 0.23 -0.6 40,766 - caiT b0040 0.23 +1.5 40,774 - caiT b0040 0.24 +0.8 40,839 - caiT b0040 0.28 +2.3 40,850 + caiT b0040 0.29 +0.2 40,889 - caiT b0040 0.31 -1.2 40,902 - caiT b0040 0.32 -1.1 40,902 - caiT b0040 0.32 -0.7 40,902 - caiT b0040 0.32 -1.9 40,908 + caiT b0040 0.32 +2.7 40,912 - caiT b0040 0.33 +0.7 40,918 - caiT b0040 0.33 +2.5 40,947 - caiT b0040 0.35 -0.2 40,947 - caiT b0040 0.35 +0.3 40,947 - caiT b0040 0.35 +1.0 40,947 - caiT b0040 0.35 -1.5 40,972 + caiT b0040 0.37 -0.5 41,029 - caiT b0040 0.40 -0.1 41,029 - caiT b0040 0.40 -0.2 41,146 + caiT b0040 0.48 -0.5 41,158 + caiT b0040 0.49 -0.4 41,158 + caiT b0040 0.49 +0.5 41,179 - caiT b0040 0.50 -0.1 41,256 - caiT b0040 0.55 -0.1 41,282 - caiT b0040 0.57 +0.2 41,318 - caiT b0040 0.59 -0.2 41,329 + caiT b0040 0.60 -0.6 41,409 + caiT b0040 0.65 +3.9 41,411 + caiT b0040 0.66 -2.0 41,417 - caiT b0040 0.66 -1.1 41,417 - caiT b0040 0.66 -1.2 41,487 - caiT b0040 0.71 -0.4 41,574 - caiT b0040 0.76 +3.1 41,709 - caiT b0040 0.85 +0.2 41,709 - caiT b0040 0.85 -0.7 41,714 + caiT b0040 0.86 -1.3 41,716 + caiT b0040 0.86 +1.3 41,716 + caiT b0040 0.86 +1.9 41,723 - caiT b0040 0.86 -0.2 41,726 + caiT b0040 0.86 +2.6 41,726 + caiT b0040 0.86 +1.3 41,734 - caiT b0040 0.87 +1.3 41,734 - caiT b0040 0.87 -0.1 41,734 - caiT b0040 0.87 -0.3 41,734 - caiT b0040 0.87 +0.3 41,734 - caiT b0040 0.87 -0.6 41,734 - caiT b0040 0.87 +1.0 41,753 + caiT b0040 0.88 -0.6 41,753 + caiT b0040 0.88 +1.2 41,753 + caiT b0040 0.88 +0.2 41,753 + caiT b0040 0.88 -0.6 41,753 + caiT b0040 0.88 +3.4 41,753 + caiT b0040 0.88 -1.1 41,757 - caiT b0040 0.88 +1.0 41,757 - caiT b0040 0.88 +0.1 41,758 + caiT b0040 0.89 +3.9 41,759 - caiT b0040 0.89 -0.2 41,761 + caiT b0040 0.89 -0.3 41,761 + caiT b0040 0.89 +1.0 41,761 - caiT b0040 0.89 -0.5 41,761 - caiT b0040 0.89 -1.1 41,771 + caiT b0040 0.89 +0.1 41,771 + caiT b0040 0.89 +4.2 41,771 + caiT b0040 0.89 +2.3 41,771 + caiT b0040 0.89 -0.9 41,771 + caiT b0040 0.89 +0.8 41,774 + caiT b0040 0.90 -1.5 41,779 + caiT b0040 0.90 -1.3 41,783 + -1.3 41,786 - +0.3 41,791 - -0.6 41,791 - +0.6 41,794 - +0.2 41,795 + -0.7 41,797 + -0.5 41,803 - +0.9 41,830 + +1.6 41,830 + +1.1 41,830 + +2.3 41,838 - +2.3 41,838 - +3.9 41,844 - +0.9 41,846 - +1.3 41,849 + -1.3 41,850 + -0.1 41,938 + +1.1 42,028 + +2.9 42,028 + +3.9 42,028 + +2.9 42,040 + +3.9 42,041 + +3.9 42,041 + +3.9 42,041 + +1.9 42,041 + +3.9 42,043 + +3.9 42,048 - -0.1 42,049 - +2.9 42,059 - +1.2 42,070 - -1.3 42,070 - -1.0 42,070 - -0.6 42,070 - +0.6 42,097 + -1.2 42,098 + -0.2 42,098 + -0.7 42,430 - +0.9 42,442 - +0.2 42,446 + +1.9 42,447 - -0.6 42,452 - -0.8 42,452 - -0.9 42,456 + +0.9 42,464 - -0.2 42,464 - -0.5 42,466 - +0.9 42,466 - -0.3 42,466 - +0.1 42,466 - +1.3 42,466 - +1.3 42,470 + +0.3 42,478 - +6.5 42,478 - +2.8 42,478 - +0.5 42,485 + fixA b0041 0.11 +1.2 42,488 - fixA b0041 0.11 +0.1 42,491 - fixA b0041 0.11 +2.5 42,491 - fixA b0041 0.11 +0.5 42,534 - fixA b0041 0.17 +0.3 42,541 + fixA b0041 0.18 -0.1 42,550 - fixA b0041 0.19 -0.6 42,591 - fixA b0041 0.24 +0.1 42,601 - fixA b0041 0.26 -0.5 42,606 - fixA b0041 0.26 +0.1 42,682 - fixA b0041 0.36 +0.9 42,745 + fixA b0041 0.44 -1.3 42,873 - fixA b0041 0.61 +0.4
Or see this region's nucleotide sequence