Experiment: PoolT9D19
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt frc and yfdX are separated by 512 nucleotides yfdX and ypdI are separated by 295 nucleotides ypdI and yfdY are separated by 76 nucleotides
b2374: frc - formyl-coenzyme A transferase (NCBI), at 2,490,026 to 2,491,276
frc
b2375: yfdX - hypothetical protein (NCBI), at 2,491,789 to 2,492,424
yfdX
b2376: ypdI - predicted lipoprotein involved in colanic acid biosynthesis (NCBI), at 2,492,720 to 2,492,995
ypdI
b2377: yfdY - predicted inner membrane protein (NCBI), at 2,493,072 to 2,493,314
yfdY
Position (kb)
2491
2492
2493 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7
8 at 2490.873 kb on - strand, within frc at 2490.875 kb on - strand, within frc at 2490.875 kb on - strand, within frc at 2490.953 kb on + strand, within frc at 2490.961 kb on - strand, within frc at 2490.962 kb on + strand, within frc at 2490.967 kb on + strand, within frc at 2490.976 kb on + strand, within frc at 2490.976 kb on + strand, within frc at 2491.026 kb on + strand, within frc at 2491.026 kb on + strand, within frc at 2491.034 kb on + strand, within frc at 2491.034 kb on + strand, within frc at 2491.082 kb on + strand, within frc at 2491.085 kb on + strand, within frc at 2491.093 kb on - strand, within frc at 2491.100 kb on + strand, within frc at 2491.100 kb on + strand, within frc at 2491.100 kb on + strand, within frc at 2491.124 kb on + strand, within frc at 2491.135 kb on + strand, within frc at 2491.135 kb on + strand, within frc at 2491.144 kb on + strand, within frc at 2491.147 kb on + strand, within frc at 2491.148 kb on + strand, within frc at 2491.158 kb on - strand at 2491.163 kb on - strand at 2491.180 kb on + strand at 2491.214 kb on + strand at 2491.214 kb on + strand at 2491.223 kb on + strand at 2491.223 kb on + strand at 2491.225 kb on + strand at 2491.225 kb on + strand at 2491.751 kb on - strand at 2491.751 kb on - strand at 2491.778 kb on - strand at 2491.778 kb on - strand at 2491.801 kb on - strand at 2491.821 kb on - strand at 2491.821 kb on - strand at 2491.905 kb on + strand, within yfdX at 2491.911 kb on - strand, within yfdX at 2491.925 kb on - strand, within yfdX at 2491.950 kb on + strand, within yfdX at 2491.956 kb on - strand, within yfdX at 2492.060 kb on - strand, within yfdX at 2492.060 kb on - strand, within yfdX at 2492.062 kb on - strand, within yfdX at 2492.102 kb on - strand, within yfdX at 2492.137 kb on - strand, within yfdX at 2492.153 kb on + strand, within yfdX at 2492.153 kb on + strand, within yfdX at 2492.159 kb on - strand, within yfdX at 2492.161 kb on - strand, within yfdX at 2492.183 kb on + strand, within yfdX at 2492.183 kb on + strand, within yfdX at 2492.221 kb on + strand, within yfdX at 2492.221 kb on + strand, within yfdX at 2492.227 kb on + strand, within yfdX at 2492.232 kb on + strand, within yfdX at 2492.269 kb on - strand, within yfdX at 2492.284 kb on - strand, within yfdX at 2492.288 kb on + strand, within yfdX at 2492.294 kb on - strand, within yfdX at 2492.327 kb on + strand, within yfdX at 2492.362 kb on + strand at 2492.362 kb on + strand at 2492.390 kb on + strand at 2492.390 kb on + strand at 2492.390 kb on + strand at 2492.409 kb on + strand at 2492.465 kb on + strand at 2492.485 kb on + strand at 2492.687 kb on - strand at 2492.771 kb on - strand, within ypdI at 2492.771 kb on - strand, within ypdI at 2492.771 kb on - strand, within ypdI at 2492.771 kb on - strand, within ypdI at 2492.818 kb on - strand, within ypdI at 2492.818 kb on - strand, within ypdI at 2492.826 kb on - strand, within ypdI at 2492.828 kb on - strand, within ypdI at 2492.848 kb on + strand, within ypdI at 2492.855 kb on + strand, within ypdI at 2492.861 kb on - strand, within ypdI at 2492.865 kb on + strand, within ypdI at 2492.873 kb on - strand, within ypdI at 2492.873 kb on - strand, within ypdI at 2492.929 kb on - strand, within ypdI at 2492.929 kb on - strand, within ypdI at 2493.005 kb on + strand at 2493.043 kb on - strand at 2493.043 kb on - strand at 2493.043 kb on - strand at 2493.056 kb on + strand at 2493.061 kb on + strand at 2493.064 kb on - strand at 2493.064 kb on - strand at 2493.075 kb on - strand at 2493.075 kb on - strand at 2493.081 kb on + strand at 2493.081 kb on + strand at 2493.095 kb on - strand at 2493.159 kb on - strand, within yfdY at 2493.159 kb on - strand, within yfdY at 2493.220 kb on + strand, within yfdY at 2493.267 kb on - strand, within yfdY at 2493.267 kb on - strand, within yfdY
Per-strain Table
Position Strand Gene LocusTag Fraction PoolT9D19 remove 2,490,873 - frc b2374 0.68 -1.8 2,490,875 - frc b2374 0.68 +0.6 2,490,875 - frc b2374 0.68 -0.4 2,490,953 + frc b2374 0.74 +0.2 2,490,961 - frc b2374 0.75 +2.2 2,490,962 + frc b2374 0.75 +0.5 2,490,967 + frc b2374 0.75 -0.3 2,490,976 + frc b2374 0.76 +0.2 2,490,976 + frc b2374 0.76 +0.1 2,491,026 + frc b2374 0.80 -0.9 2,491,026 + frc b2374 0.80 +0.3 2,491,034 + frc b2374 0.81 +1.2 2,491,034 + frc b2374 0.81 -1.0 2,491,082 + frc b2374 0.84 +0.2 2,491,085 + frc b2374 0.85 +1.5 2,491,093 - frc b2374 0.85 +2.5 2,491,100 + frc b2374 0.86 +1.5 2,491,100 + frc b2374 0.86 +1.9 2,491,100 + frc b2374 0.86 +2.5 2,491,124 + frc b2374 0.88 +3.5 2,491,135 + frc b2374 0.89 +2.5 2,491,135 + frc b2374 0.89 +2.5 2,491,144 + frc b2374 0.89 -1.1 2,491,147 + frc b2374 0.90 +1.9 2,491,148 + frc b2374 0.90 +1.5 2,491,158 - +1.9 2,491,163 - +2.1 2,491,180 + +3.5 2,491,214 + +2.5 2,491,214 + +1.9 2,491,223 + +1.1 2,491,223 + +1.2 2,491,225 + +3.5 2,491,225 + +3.5 2,491,751 - +0.7 2,491,751 - +2.5 2,491,778 - +0.7 2,491,778 - +0.3 2,491,801 - -0.0 2,491,821 - -1.6 2,491,821 - -1.7 2,491,905 + yfdX b2375 0.18 +3.5 2,491,911 - yfdX b2375 0.19 +1.9 2,491,925 - yfdX b2375 0.21 +3.5 2,491,950 + yfdX b2375 0.25 +3.5 2,491,956 - yfdX b2375 0.26 -0.0 2,492,060 - yfdX b2375 0.43 +3.5 2,492,060 - yfdX b2375 0.43 +3.5 2,492,062 - yfdX b2375 0.43 +2.5 2,492,102 - yfdX b2375 0.49 +0.5 2,492,137 - yfdX b2375 0.55 +1.0 2,492,153 + yfdX b2375 0.57 +3.5 2,492,153 + yfdX b2375 0.57 +3.5 2,492,159 - yfdX b2375 0.58 +3.0 2,492,161 - yfdX b2375 0.58 +1.4 2,492,183 + yfdX b2375 0.62 +2.5 2,492,183 + yfdX b2375 0.62 +3.5 2,492,221 + yfdX b2375 0.68 +0.3 2,492,221 + yfdX b2375 0.68 +1.5 2,492,227 + yfdX b2375 0.69 +2.5 2,492,232 + yfdX b2375 0.70 +0.9 2,492,269 - yfdX b2375 0.75 +1.5 2,492,284 - yfdX b2375 0.78 +1.3 2,492,288 + yfdX b2375 0.78 -0.4 2,492,294 - yfdX b2375 0.79 +7.9 2,492,327 + yfdX b2375 0.85 +0.1 2,492,362 + +3.5 2,492,362 + +2.5 2,492,390 + +1.2 2,492,390 + -0.5 2,492,390 + +1.5 2,492,409 + +0.9 2,492,465 + +3.5 2,492,485 + +2.5 2,492,687 - +3.5 2,492,771 - ypdI b2376 0.18 -0.1 2,492,771 - ypdI b2376 0.18 -0.4 2,492,771 - ypdI b2376 0.18 +1.4 2,492,771 - ypdI b2376 0.18 +1.0 2,492,818 - ypdI b2376 0.36 +1.1 2,492,818 - ypdI b2376 0.36 +0.2 2,492,826 - ypdI b2376 0.38 +1.5 2,492,828 - ypdI b2376 0.39 +1.8 2,492,848 + ypdI b2376 0.46 +2.5 2,492,855 + ypdI b2376 0.49 +3.5 2,492,861 - ypdI b2376 0.51 -0.7 2,492,865 + ypdI b2376 0.53 +0.3 2,492,873 - ypdI b2376 0.55 -0.4 2,492,873 - ypdI b2376 0.55 -0.4 2,492,929 - ypdI b2376 0.76 +0.5 2,492,929 - ypdI b2376 0.76 +0.2 2,493,005 + +3.5 2,493,043 - -1.1 2,493,043 - +1.2 2,493,043 - +1.8 2,493,056 + +3.5 2,493,061 + +3.5 2,493,064 - +0.1 2,493,064 - +0.4 2,493,075 - +1.4 2,493,075 - +1.2 2,493,081 + -0.3 2,493,081 + +2.5 2,493,095 - +1.3 2,493,159 - yfdY b2377 0.36 -0.4 2,493,159 - yfdY b2377 0.36 +1.6 2,493,220 + yfdY b2377 0.61 +1.5 2,493,267 - yfdY b2377 0.80 -0.5 2,493,267 - yfdY b2377 0.80 -0.0
Or see this region's nucleotide sequence