Experiment: NoPhageControl|T7phage
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrD and ycbW are separated by 173 nucleotides ycbW and ycbX overlap by 4 nucleotides
b0945: pyrD - dihydroorotate dehydrogenase (NCBI), at 1,003,991 to 1,005,001
pyrD
b0946: ycbW - orf, hypothetical protein (VIMSS), at 1,005,175 to 1,005,717
ycbW
b0947: ycbX - predicted 2Fe-2S cluster-containing protein (NCBI), at 1,005,714 to 1,006,823
ycbX
Position (kb)
1005
1006 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1004.234 kb on + strand, within pyrD at 1004.239 kb on + strand, within pyrD at 1004.256 kb on - strand, within pyrD at 1004.256 kb on - strand, within pyrD at 1004.277 kb on - strand, within pyrD at 1004.307 kb on - strand, within pyrD at 1004.350 kb on + strand, within pyrD at 1004.403 kb on - strand, within pyrD at 1004.440 kb on + strand, within pyrD at 1004.513 kb on + strand, within pyrD at 1004.513 kb on + strand, within pyrD at 1004.523 kb on + strand, within pyrD at 1004.531 kb on - strand, within pyrD at 1004.545 kb on - strand, within pyrD at 1004.553 kb on - strand, within pyrD at 1004.568 kb on + strand, within pyrD at 1004.568 kb on + strand, within pyrD at 1004.614 kb on + strand, within pyrD at 1004.636 kb on + strand, within pyrD at 1004.689 kb on - strand, within pyrD at 1004.698 kb on + strand, within pyrD at 1004.698 kb on + strand, within pyrD at 1004.708 kb on - strand, within pyrD at 1004.717 kb on + strand, within pyrD at 1004.720 kb on - strand, within pyrD at 1004.722 kb on + strand, within pyrD at 1004.727 kb on + strand, within pyrD at 1004.727 kb on + strand, within pyrD at 1004.733 kb on - strand, within pyrD at 1004.733 kb on - strand, within pyrD at 1004.736 kb on - strand, within pyrD at 1004.780 kb on - strand, within pyrD at 1004.783 kb on + strand, within pyrD at 1004.785 kb on + strand, within pyrD at 1004.791 kb on - strand, within pyrD at 1004.791 kb on - strand, within pyrD at 1004.801 kb on - strand, within pyrD at 1004.871 kb on + strand, within pyrD at 1004.871 kb on + strand, within pyrD at 1004.871 kb on + strand, within pyrD at 1004.888 kb on - strand, within pyrD at 1004.938 kb on + strand at 1004.938 kb on + strand at 1005.020 kb on - strand at 1005.067 kb on - strand at 1005.108 kb on - strand at 1005.124 kb on + strand at 1005.137 kb on - strand at 1005.150 kb on + strand at 1005.152 kb on + strand at 1005.160 kb on + strand at 1005.166 kb on - strand at 1005.166 kb on - strand at 1005.168 kb on - strand at 1005.168 kb on - strand at 1005.170 kb on - strand at 1005.215 kb on - strand at 1005.227 kb on + strand at 1005.228 kb on - strand at 1005.228 kb on - strand at 1005.261 kb on - strand, within ycbW at 1005.331 kb on - strand, within ycbW at 1005.405 kb on + strand, within ycbW at 1005.408 kb on + strand, within ycbW at 1005.408 kb on + strand, within ycbW at 1005.413 kb on - strand, within ycbW at 1005.413 kb on - strand, within ycbW at 1005.414 kb on - strand, within ycbW at 1005.414 kb on - strand, within ycbW at 1005.425 kb on - strand, within ycbW at 1005.515 kb on + strand, within ycbW at 1005.522 kb on + strand, within ycbW at 1005.628 kb on + strand, within ycbW at 1005.712 kb on + strand at 1005.720 kb on - strand at 1005.973 kb on - strand, within ycbX at 1005.981 kb on - strand, within ycbX at 1006.113 kb on + strand, within ycbX at 1006.113 kb on + strand, within ycbX at 1006.191 kb on - strand, within ycbX at 1006.274 kb on - strand, within ycbX at 1006.280 kb on - strand, within ycbX at 1006.280 kb on - strand, within ycbX at 1006.309 kb on - strand, within ycbX at 1006.402 kb on + strand, within ycbX at 1006.610 kb on - strand, within ycbX at 1006.648 kb on + strand, within ycbX
Per-strain Table
Position Strand Gene LocusTag Fraction NoPhageControl|T7phage remove 1,004,234 + pyrD b0945 0.24 -0.8 1,004,239 + pyrD b0945 0.25 -0.3 1,004,256 - pyrD b0945 0.26 -0.9 1,004,256 - pyrD b0945 0.26 +1.0 1,004,277 - pyrD b0945 0.28 -0.3 1,004,307 - pyrD b0945 0.31 -2.5 1,004,350 + pyrD b0945 0.36 -0.4 1,004,403 - pyrD b0945 0.41 +0.5 1,004,440 + pyrD b0945 0.44 -0.5 1,004,513 + pyrD b0945 0.52 +0.4 1,004,513 + pyrD b0945 0.52 -1.3 1,004,523 + pyrD b0945 0.53 +0.4 1,004,531 - pyrD b0945 0.53 +0.5 1,004,545 - pyrD b0945 0.55 +1.5 1,004,553 - pyrD b0945 0.56 +0.3 1,004,568 + pyrD b0945 0.57 +0.4 1,004,568 + pyrD b0945 0.57 -1.8 1,004,614 + pyrD b0945 0.62 -0.1 1,004,636 + pyrD b0945 0.64 +0.6 1,004,689 - pyrD b0945 0.69 -0.1 1,004,698 + pyrD b0945 0.70 -0.1 1,004,698 + pyrD b0945 0.70 -0.6 1,004,708 - pyrD b0945 0.71 -1.4 1,004,717 + pyrD b0945 0.72 -0.6 1,004,720 - pyrD b0945 0.72 +0.1 1,004,722 + pyrD b0945 0.72 +0.4 1,004,727 + pyrD b0945 0.73 -0.4 1,004,727 + pyrD b0945 0.73 +0.2 1,004,733 - pyrD b0945 0.73 +0.4 1,004,733 - pyrD b0945 0.73 -1.0 1,004,736 - pyrD b0945 0.74 +0.1 1,004,780 - pyrD b0945 0.78 -1.6 1,004,783 + pyrD b0945 0.78 -0.3 1,004,785 + pyrD b0945 0.79 -1.4 1,004,791 - pyrD b0945 0.79 -2.3 1,004,791 - pyrD b0945 0.79 +0.1 1,004,801 - pyrD b0945 0.80 +0.3 1,004,871 + pyrD b0945 0.87 -0.9 1,004,871 + pyrD b0945 0.87 -0.7 1,004,871 + pyrD b0945 0.87 -0.8 1,004,888 - pyrD b0945 0.89 -1.1 1,004,938 + -0.4 1,004,938 + +0.2 1,005,020 - +1.3 1,005,067 - -1.4 1,005,108 - +0.1 1,005,124 + +0.9 1,005,137 - +1.3 1,005,150 + +1.5 1,005,152 + +1.4 1,005,160 + -1.5 1,005,166 - +1.0 1,005,166 - +0.0 1,005,168 - -0.4 1,005,168 - -0.4 1,005,170 - -0.6 1,005,215 - +1.0 1,005,227 + -2.1 1,005,228 - -0.5 1,005,228 - +2.9 1,005,261 - ycbW b0946 0.16 -2.4 1,005,331 - ycbW b0946 0.29 -1.8 1,005,405 + ycbW b0946 0.42 -0.3 1,005,408 + ycbW b0946 0.43 +0.3 1,005,408 + ycbW b0946 0.43 +0.3 1,005,413 - ycbW b0946 0.44 +0.2 1,005,413 - ycbW b0946 0.44 +0.2 1,005,414 - ycbW b0946 0.44 -1.7 1,005,414 - ycbW b0946 0.44 -1.0 1,005,425 - ycbW b0946 0.46 -0.2 1,005,515 + ycbW b0946 0.63 -0.2 1,005,522 + ycbW b0946 0.64 -1.5 1,005,628 + ycbW b0946 0.83 +0.2 1,005,712 + -0.1 1,005,720 - +0.9 1,005,973 - ycbX b0947 0.23 -0.4 1,005,981 - ycbX b0947 0.24 -1.3 1,006,113 + ycbX b0947 0.36 -0.2 1,006,113 + ycbX b0947 0.36 -1.4 1,006,191 - ycbX b0947 0.43 -0.9 1,006,274 - ycbX b0947 0.50 +0.1 1,006,280 - ycbX b0947 0.51 +0.4 1,006,280 - ycbX b0947 0.51 +0.8 1,006,309 - ycbX b0947 0.54 +0.3 1,006,402 + ycbX b0947 0.62 +0.5 1,006,610 - ycbX b0947 0.81 -0.0 1,006,648 + ycbX b0947 0.84 -0.5
Or see this region's nucleotide sequence