Experiment: NoPhageControl|T7phage
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nudH and mutH are separated by 684 nucleotides mutH and ygdQ are separated by 68 nucleotides ygdQ and ygdR are separated by 137 nucleotides
b2830: nudH - dinucleoside polyphosphate hydrolase (NCBI), at 2,966,469 to 2,966,999
nudH
b2831: mutH - DNA mismatch repair protein (NCBI), at 2,967,684 to 2,968,373
mutH
b2832: ygdQ - predicted inner membrane protein (NCBI), at 2,968,442 to 2,969,155
ygdQ
b2833: ygdR - hypothetical protein (NCBI), at 2,969,293 to 2,969,511
ygdR
Position (kb)
2967
2968
2969 Strain fitness (log2 ratio)
-1
0
1
2 at 2966.722 kb on + strand, within nudH at 2967.071 kb on + strand at 2967.078 kb on + strand at 2967.078 kb on + strand at 2967.640 kb on - strand at 2967.694 kb on - strand at 2967.728 kb on + strand at 2967.768 kb on - strand, within mutH at 2967.768 kb on - strand, within mutH at 2967.994 kb on - strand, within mutH at 2968.044 kb on + strand, within mutH at 2968.088 kb on + strand, within mutH at 2968.088 kb on + strand, within mutH at 2968.096 kb on - strand, within mutH at 2968.174 kb on + strand, within mutH at 2968.179 kb on - strand, within mutH at 2968.183 kb on - strand, within mutH at 2968.499 kb on + strand at 2968.509 kb on - strand at 2968.509 kb on - strand at 2968.614 kb on - strand, within ygdQ at 2968.614 kb on - strand, within ygdQ at 2968.620 kb on - strand, within ygdQ at 2968.620 kb on - strand, within ygdQ at 2968.648 kb on + strand, within ygdQ at 2968.648 kb on + strand, within ygdQ at 2968.674 kb on + strand, within ygdQ at 2968.786 kb on - strand, within ygdQ at 2968.829 kb on + strand, within ygdQ at 2968.856 kb on - strand, within ygdQ at 2968.880 kb on + strand, within ygdQ at 2968.942 kb on + strand, within ygdQ at 2968.942 kb on + strand, within ygdQ at 2968.955 kb on - strand, within ygdQ at 2969.039 kb on - strand, within ygdQ at 2969.039 kb on - strand, within ygdQ at 2969.108 kb on - strand at 2969.108 kb on - strand at 2969.160 kb on - strand at 2969.160 kb on - strand at 2969.164 kb on + strand at 2969.172 kb on - strand at 2969.172 kb on - strand at 2969.193 kb on + strand at 2969.193 kb on - strand at 2969.202 kb on - strand at 2969.231 kb on + strand at 2969.242 kb on - strand at 2969.263 kb on + strand at 2969.263 kb on + strand at 2969.280 kb on - strand at 2969.281 kb on - strand at 2969.286 kb on - strand at 2969.327 kb on + strand, within ygdR
Per-strain Table
Position Strand Gene LocusTag Fraction NoPhageControl|T7phage remove 2,966,722 + nudH b2830 0.48 +2.4 2,967,071 + -0.4 2,967,078 + -0.3 2,967,078 + +0.3 2,967,640 - -0.6 2,967,694 - +2.4 2,967,728 + +0.4 2,967,768 - mutH b2831 0.12 +1.4 2,967,768 - mutH b2831 0.12 +2.0 2,967,994 - mutH b2831 0.45 +0.7 2,968,044 + mutH b2831 0.52 -0.5 2,968,088 + mutH b2831 0.59 +0.6 2,968,088 + mutH b2831 0.59 +1.0 2,968,096 - mutH b2831 0.60 +0.2 2,968,174 + mutH b2831 0.71 +0.4 2,968,179 - mutH b2831 0.72 +0.3 2,968,183 - mutH b2831 0.72 -0.3 2,968,499 + -1.2 2,968,509 - -0.3 2,968,509 - -0.2 2,968,614 - ygdQ b2832 0.24 +0.0 2,968,614 - ygdQ b2832 0.24 +0.3 2,968,620 - ygdQ b2832 0.25 +0.4 2,968,620 - ygdQ b2832 0.25 +1.0 2,968,648 + ygdQ b2832 0.29 -0.0 2,968,648 + ygdQ b2832 0.29 +0.1 2,968,674 + ygdQ b2832 0.32 -0.4 2,968,786 - ygdQ b2832 0.48 -0.0 2,968,829 + ygdQ b2832 0.54 +0.3 2,968,856 - ygdQ b2832 0.58 +0.6 2,968,880 + ygdQ b2832 0.61 +0.2 2,968,942 + ygdQ b2832 0.70 -0.3 2,968,942 + ygdQ b2832 0.70 -0.4 2,968,955 - ygdQ b2832 0.72 -0.9 2,969,039 - ygdQ b2832 0.84 -1.0 2,969,039 - ygdQ b2832 0.84 +0.4 2,969,108 - +0.1 2,969,108 - +0.8 2,969,160 - +0.2 2,969,160 - +0.1 2,969,164 + +0.7 2,969,172 - +0.0 2,969,172 - -0.4 2,969,193 + -0.2 2,969,193 - -0.4 2,969,202 - -0.8 2,969,231 + -0.3 2,969,242 - +0.6 2,969,263 + +0.7 2,969,263 + +1.0 2,969,280 - +0.2 2,969,281 - -0.3 2,969,286 - -0.4 2,969,327 + ygdR b2833 0.16 -0.3
Or see this region's nucleotide sequence