Experiment: NoPhageControl|T7phage
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ansA and ydjB are separated by 10 nucleotides ydjB and ydjE are separated by 92 nucleotides ydjE and ydjF are separated by 116 nucleotides
b1767: ansA - cytoplasmic asparaginase I (NCBI), at 1,848,884 to 1,849,900
ansA
b1768: ydjB - orf, hypothetical protein (VIMSS), at 1,849,911 to 1,850,552
ydjB
b1769: ydjE - predicted transporter (NCBI), at 1,850,645 to 1,852,003
ydjE
b1770: ydjF - predicted DNA-binding transcriptional regulator (NCBI), at 1,852,120 to 1,852,878
ydjF
Position (kb)
1850
1851
1852
1853 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1849.751 kb on + strand, within ansA at 1849.785 kb on + strand, within ansA at 1849.896 kb on + strand at 1849.896 kb on + strand at 1850.057 kb on - strand, within ydjB at 1850.262 kb on + strand, within ydjB at 1850.272 kb on - strand, within ydjB at 1850.313 kb on + strand, within ydjB at 1850.352 kb on + strand, within ydjB at 1850.353 kb on + strand, within ydjB at 1850.365 kb on + strand, within ydjB at 1850.365 kb on + strand, within ydjB at 1850.367 kb on + strand, within ydjB at 1850.372 kb on + strand, within ydjB at 1850.372 kb on + strand, within ydjB at 1850.463 kb on + strand, within ydjB at 1850.463 kb on + strand, within ydjB at 1850.463 kb on + strand, within ydjB at 1850.484 kb on + strand, within ydjB at 1850.523 kb on + strand at 1850.552 kb on + strand at 1850.552 kb on + strand at 1850.567 kb on + strand at 1850.567 kb on + strand at 1850.570 kb on + strand at 1850.570 kb on + strand at 1850.669 kb on + strand at 1850.718 kb on - strand at 1850.718 kb on - strand at 1850.720 kb on + strand at 1850.728 kb on + strand at 1850.728 kb on + strand at 1850.728 kb on - strand at 1850.738 kb on + strand at 1850.752 kb on - strand at 1850.795 kb on - strand, within ydjE at 1850.795 kb on - strand, within ydjE at 1850.795 kb on - strand, within ydjE at 1850.796 kb on + strand, within ydjE at 1850.796 kb on + strand, within ydjE at 1850.803 kb on + strand, within ydjE at 1850.804 kb on - strand, within ydjE at 1850.812 kb on - strand, within ydjE at 1850.816 kb on - strand, within ydjE at 1850.836 kb on + strand, within ydjE at 1850.851 kb on + strand, within ydjE at 1850.852 kb on + strand, within ydjE at 1850.853 kb on + strand, within ydjE at 1850.854 kb on + strand, within ydjE at 1850.907 kb on + strand, within ydjE at 1850.907 kb on + strand, within ydjE at 1850.942 kb on + strand, within ydjE at 1850.957 kb on + strand, within ydjE at 1850.978 kb on + strand, within ydjE at 1851.163 kb on - strand, within ydjE at 1851.176 kb on - strand, within ydjE at 1851.276 kb on - strand, within ydjE at 1851.280 kb on - strand, within ydjE at 1851.297 kb on - strand, within ydjE at 1851.312 kb on - strand, within ydjE at 1851.312 kb on - strand, within ydjE at 1851.348 kb on - strand, within ydjE at 1851.348 kb on - strand, within ydjE at 1851.364 kb on - strand, within ydjE at 1851.435 kb on - strand, within ydjE at 1851.457 kb on - strand, within ydjE at 1851.521 kb on - strand, within ydjE at 1851.521 kb on - strand, within ydjE at 1851.526 kb on - strand, within ydjE at 1851.666 kb on + strand, within ydjE at 1851.668 kb on + strand, within ydjE at 1851.671 kb on + strand, within ydjE at 1851.671 kb on + strand, within ydjE at 1851.679 kb on - strand, within ydjE at 1851.703 kb on + strand, within ydjE at 1851.716 kb on + strand, within ydjE at 1851.736 kb on + strand, within ydjE at 1851.769 kb on + strand, within ydjE at 1851.774 kb on + strand, within ydjE at 1851.832 kb on + strand, within ydjE at 1851.877 kb on + strand at 1851.968 kb on + strand at 1851.968 kb on + strand at 1851.976 kb on - strand at 1852.035 kb on + strand at 1852.163 kb on - strand at 1852.165 kb on - strand at 1852.165 kb on - strand at 1852.165 kb on - strand at 1852.167 kb on - strand at 1852.172 kb on - strand at 1852.224 kb on - strand, within ydjF at 1852.252 kb on - strand, within ydjF at 1852.252 kb on - strand, within ydjF at 1852.252 kb on - strand, within ydjF at 1852.252 kb on - strand, within ydjF at 1852.252 kb on - strand, within ydjF at 1852.264 kb on - strand, within ydjF at 1852.264 kb on - strand, within ydjF at 1852.388 kb on - strand, within ydjF at 1852.457 kb on - strand, within ydjF at 1852.527 kb on + strand, within ydjF at 1852.532 kb on + strand, within ydjF at 1852.535 kb on - strand, within ydjF at 1852.535 kb on - strand, within ydjF at 1852.535 kb on - strand, within ydjF at 1852.576 kb on - strand, within ydjF at 1852.581 kb on - strand, within ydjF at 1852.606 kb on + strand, within ydjF at 1852.611 kb on - strand, within ydjF at 1852.636 kb on - strand, within ydjF at 1852.853 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction NoPhageControl|T7phage remove 1,849,751 + ansA b1767 0.85 -0.5 1,849,785 + ansA b1767 0.89 +0.5 1,849,896 + -0.3 1,849,896 + +0.2 1,850,057 - ydjB b1768 0.23 -0.8 1,850,262 + ydjB b1768 0.55 -0.3 1,850,272 - ydjB b1768 0.56 +0.2 1,850,313 + ydjB b1768 0.63 -1.0 1,850,352 + ydjB b1768 0.69 +0.7 1,850,353 + ydjB b1768 0.69 -0.0 1,850,365 + ydjB b1768 0.71 +0.2 1,850,365 + ydjB b1768 0.71 +0.1 1,850,367 + ydjB b1768 0.71 +0.2 1,850,372 + ydjB b1768 0.72 +0.4 1,850,372 + ydjB b1768 0.72 +0.8 1,850,463 + ydjB b1768 0.86 +0.3 1,850,463 + ydjB b1768 0.86 -1.2 1,850,463 + ydjB b1768 0.86 -0.7 1,850,484 + ydjB b1768 0.89 +0.1 1,850,523 + -0.3 1,850,552 + +0.1 1,850,552 + -0.2 1,850,567 + +1.5 1,850,567 + -1.0 1,850,570 + -0.9 1,850,570 + +0.4 1,850,669 + -2.6 1,850,718 - +0.0 1,850,718 - -1.5 1,850,720 + -1.8 1,850,728 + +1.3 1,850,728 + +0.6 1,850,728 - +0.2 1,850,738 + +1.9 1,850,752 - -0.5 1,850,795 - ydjE b1769 0.11 +0.1 1,850,795 - ydjE b1769 0.11 +0.9 1,850,795 - ydjE b1769 0.11 +0.7 1,850,796 + ydjE b1769 0.11 +1.1 1,850,796 + ydjE b1769 0.11 +1.8 1,850,803 + ydjE b1769 0.12 -0.2 1,850,804 - ydjE b1769 0.12 -1.3 1,850,812 - ydjE b1769 0.12 +0.5 1,850,816 - ydjE b1769 0.13 +0.1 1,850,836 + ydjE b1769 0.14 -1.0 1,850,851 + ydjE b1769 0.15 +2.5 1,850,852 + ydjE b1769 0.15 +2.5 1,850,853 + ydjE b1769 0.15 +2.1 1,850,854 + ydjE b1769 0.15 +2.1 1,850,907 + ydjE b1769 0.19 +1.1 1,850,907 + ydjE b1769 0.19 +4.1 1,850,942 + ydjE b1769 0.22 +1.1 1,850,957 + ydjE b1769 0.23 +0.1 1,850,978 + ydjE b1769 0.25 +2.1 1,851,163 - ydjE b1769 0.38 -0.7 1,851,176 - ydjE b1769 0.39 +2.1 1,851,276 - ydjE b1769 0.46 -0.9 1,851,280 - ydjE b1769 0.47 +0.1 1,851,297 - ydjE b1769 0.48 +2.1 1,851,312 - ydjE b1769 0.49 +2.1 1,851,312 - ydjE b1769 0.49 +3.1 1,851,348 - ydjE b1769 0.52 +4.4 1,851,348 - ydjE b1769 0.52 +1.1 1,851,364 - ydjE b1769 0.53 -0.1 1,851,435 - ydjE b1769 0.58 +1.1 1,851,457 - ydjE b1769 0.60 +2.1 1,851,521 - ydjE b1769 0.64 +1.5 1,851,521 - ydjE b1769 0.64 +0.1 1,851,526 - ydjE b1769 0.65 +2.2 1,851,666 + ydjE b1769 0.75 +0.1 1,851,668 + ydjE b1769 0.75 +0.1 1,851,671 + ydjE b1769 0.75 +0.3 1,851,671 + ydjE b1769 0.75 +3.7 1,851,679 - ydjE b1769 0.76 +3.1 1,851,703 + ydjE b1769 0.78 +2.3 1,851,716 + ydjE b1769 0.79 -0.2 1,851,736 + ydjE b1769 0.80 +0.4 1,851,769 + ydjE b1769 0.83 -0.2 1,851,774 + ydjE b1769 0.83 +0.2 1,851,832 + ydjE b1769 0.87 +0.4 1,851,877 + -0.0 1,851,968 + -0.5 1,851,968 + +0.8 1,851,976 - +2.1 1,852,035 + -0.0 1,852,163 - +0.8 1,852,165 - -0.7 1,852,165 - -0.8 1,852,165 - +0.0 1,852,167 - -1.0 1,852,172 - +0.3 1,852,224 - ydjF b1770 0.14 -0.2 1,852,252 - ydjF b1770 0.17 +0.5 1,852,252 - ydjF b1770 0.17 +0.1 1,852,252 - ydjF b1770 0.17 +1.2 1,852,252 - ydjF b1770 0.17 -0.3 1,852,252 - ydjF b1770 0.17 -2.7 1,852,264 - ydjF b1770 0.19 +0.3 1,852,264 - ydjF b1770 0.19 +0.1 1,852,388 - ydjF b1770 0.35 -0.2 1,852,457 - ydjF b1770 0.44 -0.6 1,852,527 + ydjF b1770 0.54 -0.5 1,852,532 + ydjF b1770 0.54 +2.1 1,852,535 - ydjF b1770 0.55 +0.3 1,852,535 - ydjF b1770 0.55 +0.3 1,852,535 - ydjF b1770 0.55 +1.2 1,852,576 - ydjF b1770 0.60 +0.1 1,852,581 - ydjF b1770 0.61 -0.3 1,852,606 + ydjF b1770 0.64 +2.1 1,852,611 - ydjF b1770 0.65 +0.3 1,852,636 - ydjF b1770 0.68 +0.8 1,852,853 + +1.1
Or see this region's nucleotide sequence