Experiment: NoPhageControl|T7phage
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt paaC and paaD are separated by 14 nucleotides paaD and paaE are separated by 7 nucleotides paaE and paaF overlap by 4 nucleotides paaF and paaG overlap by 1 nucleotides
b1390: paaC - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI), at 1,453,188 to 1,453,934
paaC
b1391: paaD - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (RefSeq), at 1,453,949 to 1,454,446
paaD
b1392: paaE - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI), at 1,454,454 to 1,455,524
paaE
b1393: paaF - enoyl-CoA hydratase-isomerase (NCBI), at 1,455,521 to 1,456,288
paaF
b1394: paaG - enoyl-CoA hydratase (NCBI), at 1,456,288 to 1,457,076
paaG
Position (kb)
1454
1455
1456 Strain fitness (log2 ratio)
-2
-1
0
1 at 1453.483 kb on - strand, within paaC at 1453.635 kb on - strand, within paaC at 1453.641 kb on + strand, within paaC at 1453.689 kb on + strand, within paaC at 1453.840 kb on - strand, within paaC at 1454.381 kb on + strand, within paaD at 1454.381 kb on + strand, within paaD at 1454.398 kb on - strand at 1454.470 kb on + strand at 1454.532 kb on - strand at 1454.714 kb on + strand, within paaE at 1454.714 kb on + strand, within paaE at 1454.786 kb on - strand, within paaE at 1455.114 kb on + strand, within paaE at 1455.315 kb on + strand, within paaE at 1455.392 kb on - strand, within paaE at 1455.429 kb on - strand at 1455.610 kb on + strand, within paaF at 1456.073 kb on + strand, within paaF at 1456.216 kb on - strand at 1456.216 kb on - strand at 1456.490 kb on + strand, within paaG
Per-strain Table
Position Strand Gene LocusTag Fraction NoPhageControl|T7phage remove 1,453,483 - paaC b1390 0.39 +0.6 1,453,635 - paaC b1390 0.60 -1.8 1,453,641 + paaC b1390 0.61 -1.1 1,453,689 + paaC b1390 0.67 -0.2 1,453,840 - paaC b1390 0.87 +0.3 1,454,381 + paaD b1391 0.87 +1.2 1,454,381 + paaD b1391 0.87 +0.2 1,454,398 - -0.0 1,454,470 + +0.1 1,454,532 - +0.4 1,454,714 + paaE b1392 0.24 -0.7 1,454,714 + paaE b1392 0.24 +0.6 1,454,786 - paaE b1392 0.31 +1.2 1,455,114 + paaE b1392 0.62 -0.2 1,455,315 + paaE b1392 0.80 +0.2 1,455,392 - paaE b1392 0.88 +0.8 1,455,429 - -1.3 1,455,610 + paaF b1393 0.12 -0.8 1,456,073 + paaF b1393 0.72 -0.8 1,456,216 - -0.6 1,456,216 - +0.4 1,456,490 + paaG b1394 0.26 +0.6
Or see this region's nucleotide sequence