Experiment: NoPhageControl|T7phage
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rluC and yceF are separated by 111 nucleotides yceF and sraB overlap by 7 nucleotides sraB and yceD are separated by 36 nucleotides yceD and rpmF are separated by 51 nucleotides rpmF and plsX are separated by 80 nucleotides
b1086: rluC - 23S rRNA pseudouridylate synthase (NCBI), at 1,144,163 to 1,145,122
rluC
b1087: yceF - orf, hypothetical protein (VIMSS), at 1,145,234 to 1,145,818
yceF
b4418: sraB - unknown RNA (NCBI), at 1,145,812 to 1,145,980
sraB
b1088: yceD - hypothetical protein (NCBI), at 1,146,017 to 1,146,538
yceD
b1089: rpmF - 50S ribosomal protein L32 (NCBI), at 1,146,590 to 1,146,763
rpmF
b1090: plsX - glycerolphosphate auxotrophy in plsB background (VIMSS), at 1,146,844 to 1,147,914
plsX
Position (kb)
1146
1147 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1145.035 kb on + strand at 1145.058 kb on - strand at 1145.145 kb on - strand at 1145.279 kb on - strand at 1145.381 kb on + strand, within yceF at 1145.381 kb on + strand, within yceF at 1145.381 kb on + strand, within yceF at 1145.381 kb on + strand, within yceF at 1145.389 kb on - strand, within yceF at 1145.389 kb on - strand, within yceF at 1145.389 kb on - strand, within yceF at 1145.395 kb on + strand, within yceF at 1145.511 kb on - strand, within yceF at 1145.516 kb on - strand, within yceF at 1145.516 kb on - strand, within yceF at 1145.576 kb on - strand, within yceF at 1145.610 kb on - strand, within yceF at 1145.614 kb on - strand, within yceF at 1145.673 kb on - strand, within yceF at 1145.724 kb on - strand, within yceF at 1145.734 kb on - strand, within yceF at 1145.734 kb on - strand, within yceF at 1145.841 kb on + strand, within sraB at 1145.847 kb on - strand, within sraB at 1146.042 kb on + strand at 1146.052 kb on + strand at 1146.096 kb on + strand, within yceD at 1146.096 kb on + strand, within yceD at 1146.117 kb on + strand, within yceD at 1146.265 kb on + strand, within yceD at 1146.265 kb on + strand, within yceD at 1146.265 kb on + strand, within yceD at 1146.265 kb on + strand, within yceD at 1146.277 kb on + strand, within yceD at 1146.277 kb on + strand, within yceD at 1146.310 kb on + strand, within yceD at 1146.373 kb on + strand, within yceD at 1146.373 kb on + strand, within yceD at 1146.987 kb on + strand, within plsX at 1147.009 kb on - strand, within plsX at 1147.019 kb on - strand, within plsX at 1147.019 kb on - strand, within plsX at 1147.059 kb on + strand, within plsX at 1147.130 kb on - strand, within plsX at 1147.298 kb on + strand, within plsX at 1147.298 kb on + strand, within plsX at 1147.385 kb on - strand, within plsX at 1147.385 kb on - strand, within plsX at 1147.429 kb on + strand, within plsX
Per-strain Table
Position Strand Gene LocusTag Fraction NoPhageControl|T7phage remove 1,145,035 + -0.5 1,145,058 - +0.8 1,145,145 - -0.2 1,145,279 - -0.1 1,145,381 + yceF b1087 0.25 +0.1 1,145,381 + yceF b1087 0.25 -0.4 1,145,381 + yceF b1087 0.25 +0.2 1,145,381 + yceF b1087 0.25 -0.3 1,145,389 - yceF b1087 0.26 +0.2 1,145,389 - yceF b1087 0.26 -0.1 1,145,389 - yceF b1087 0.26 -1.0 1,145,395 + yceF b1087 0.28 +2.2 1,145,511 - yceF b1087 0.47 -0.1 1,145,516 - yceF b1087 0.48 +1.7 1,145,516 - yceF b1087 0.48 -1.3 1,145,576 - yceF b1087 0.58 +0.1 1,145,610 - yceF b1087 0.64 -0.0 1,145,614 - yceF b1087 0.65 +0.0 1,145,673 - yceF b1087 0.75 -0.4 1,145,724 - yceF b1087 0.84 +0.3 1,145,734 - yceF b1087 0.85 -0.1 1,145,734 - yceF b1087 0.85 +0.6 1,145,841 + sraB b4418 0.17 +0.1 1,145,847 - sraB b4418 0.21 +1.2 1,146,042 + +0.3 1,146,052 + +0.6 1,146,096 + yceD b1088 0.15 +0.2 1,146,096 + yceD b1088 0.15 +0.7 1,146,117 + yceD b1088 0.19 +1.6 1,146,265 + yceD b1088 0.48 -0.7 1,146,265 + yceD b1088 0.48 +0.5 1,146,265 + yceD b1088 0.48 +0.3 1,146,265 + yceD b1088 0.48 -0.8 1,146,277 + yceD b1088 0.50 +0.6 1,146,277 + yceD b1088 0.50 +0.1 1,146,310 + yceD b1088 0.56 -0.0 1,146,373 + yceD b1088 0.68 +1.3 1,146,373 + yceD b1088 0.68 +2.1 1,146,987 + plsX b1090 0.13 +1.2 1,147,009 - plsX b1090 0.15 -0.1 1,147,019 - plsX b1090 0.16 -0.5 1,147,019 - plsX b1090 0.16 -0.0 1,147,059 + plsX b1090 0.20 +0.1 1,147,130 - plsX b1090 0.27 -0.1 1,147,298 + plsX b1090 0.42 +2.0 1,147,298 + plsX b1090 0.42 -0.6 1,147,385 - plsX b1090 0.51 +0.1 1,147,385 - plsX b1090 0.51 -2.9 1,147,429 + plsX b1090 0.55 -1.3
Or see this region's nucleotide sequence