Strain Fitness in Escherichia coli BW25113 around b4074

Experiment: HSDP4

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	HSDP4
4,288,543	-				+0.2
4,288,617	-	nrfD	b4073	0.12	-3.1
4,288,617	-	nrfD	b4073	0.12	+1.0
4,288,650	-	nrfD	b4073	0.16	-0.5
4,288,650	-	nrfD	b4073	0.16	+2.0
4,288,655	-	nrfD	b4073	0.16	+0.6
4,288,732	+	nrfD	b4073	0.24	-3.5
4,288,740	+	nrfD	b4073	0.25	+1.0
4,288,804	+	nrfD	b4073	0.32	-3.4
4,288,804	+	nrfD	b4073	0.32	+1.3
4,288,807	+	nrfD	b4073	0.32	+0.4
4,288,825	-	nrfD	b4073	0.34	+1.5
4,288,825	-	nrfD	b4073	0.34	-3.2
4,288,888	+	nrfD	b4073	0.41	-2.7
4,288,972	+	nrfD	b4073	0.49	-2.6
4,288,972	+	nrfD	b4073	0.49	-2.4
4,288,978	+	nrfD	b4073	0.50	+2.0
4,288,978	+	nrfD	b4073	0.50	-3.7
4,289,100	-	nrfD	b4073	0.63	+0.8
4,289,154	+	nrfD	b4073	0.68	-2.7
4,289,154	+				-2.7
4,289,163	+	nrfD	b4073	0.69	-2.2
4,289,170	+	nrfD	b4073	0.70	+1.7
4,289,170	+	nrfD	b4073	0.70	+0.3
4,289,324	+	nrfD	b4073	0.86	+1.7
4,289,324	+	nrfD	b4073	0.86	+0.1
4,289,324	+	nrfD	b4073	0.86	-1.9
4,289,361	+				-2.6
4,289,380	+				-3.7
4,289,543	-				+0.3
4,289,576	-				+1.1
4,289,621	+				-3.8
4,289,621	+				-3.3
4,289,647	+				+0.2
4,289,707	+	nrfE	b4074	0.10	-2.0
4,289,707	+	nrfE	b4074	0.10	-2.1
4,289,786	+	nrfE	b4074	0.15	+0.8
4,289,896	+	nrfE	b4074	0.22	-3.0
4,289,914	-	nrfE	b4074	0.23	+1.0
4,289,914	-	nrfE	b4074	0.23	-2.3
4,289,932	+	nrfE	b4074	0.24	-2.8
4,289,932	+				-1.6
4,289,985	+	nrfE	b4074	0.27	+0.3
4,289,985	+	nrfE	b4074	0.27	-1.3
4,290,140	+	nrfE	b4074	0.36	+0.4
4,290,255	+	nrfE	b4074	0.43	+1.0
4,290,255	+	nrfE	b4074	0.43	+0.4
4,290,265	-	nrfE	b4074	0.44	-2.1
4,290,415	-	nrfE	b4074	0.53	+0.1
4,290,415	-	nrfE	b4074	0.53	+0.2
4,290,441	-	nrfE	b4074	0.55	-0.5
4,290,443	+	nrfE	b4074	0.55	-4.3
4,290,443	+	nrfE	b4074	0.55	-4.1
4,290,465	+	nrfE	b4074	0.56	+0.2
4,290,465	-	nrfE	b4074	0.56	+0.7
4,290,465	-	nrfE	b4074	0.56	-1.6
4,290,566	+	nrfE	b4074	0.62	-0.5
4,290,572	-	nrfE	b4074	0.63	-3.0
4,290,619	+	nrfE	b4074	0.65	-4.5
4,290,619	+	nrfE	b4074	0.65	+0.0
4,290,726	+	nrfE	b4074	0.72	-0.7
4,290,797	-	nrfE	b4074	0.76	-0.5
4,290,832	+	nrfE	b4074	0.78	-1.6
4,290,839	+	nrfE	b4074	0.79	+1.2
4,290,839	+	nrfE	b4074	0.79	-2.4
4,290,847	-	nrfE	b4074	0.79	+0.2
4,290,930	+	nrfE	b4074	0.84	-4.5
4,290,952	+	nrfE	b4074	0.85	-2.9
4,291,075	+				-0.5
4,291,075	+				+0.9
4,291,155	+				-0.6
4,291,208	-				+0.9
4,291,236	+	nrfF	b4075	0.13	+1.4
4,291,270	+	nrfF	b4075	0.22	-0.5
4,291,423	-	nrfF	b4075	0.62	-3.7
4,291,464	+	nrfF	b4075	0.72	-0.4
4,291,472	-	nrfF	b4075	0.74	-4.0
4,291,483	+	nrfF	b4075	0.77	+0.8
4,291,497	+	nrfF	b4075	0.81	+0.4
4,291,503	+	nrfF	b4075	0.83	-3.9
4,291,503	+	nrfF	b4075	0.83	-0.1
4,291,711	-	nrfG	b4076	0.24	-0.2
4,291,800	-	nrfG	b4076	0.39	-3.7
4,291,854	+	nrfG	b4076	0.48	-3.5
4,291,859	+	nrfG	b4076	0.49	+0.7
4,291,911	+	nrfG	b4076	0.58	-4.0
4,291,911	+	nrfG	b4076	0.58	-1.2
4,291,934	+	nrfG	b4076	0.62	-3.5
4,291,982	+	nrfG	b4076	0.70	-0.7
4,291,982	+	nrfG	b4076	0.70	+0.1
4,292,106	+				+1.2
4,292,109	+				-0.2
4,292,112	-				-0.1
4,292,112	-				-3.6
4,292,114	+				-2.7
4,292,114	+				-3.5
4,292,131	-				-1.5