Experiment: HSDP4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpoD and ygjF are separated by 78 nucleotides ygjF and ileX are separated by 124 nucleotides ileX and yqjH are separated by 53 nucleotides yqjH and yqjI are separated by 287 nucleotides
b3067: rpoD - RNA polymerase sigma factor (NCBI), at 3,211,069 to 3,212,910
rpoD
b3068: ygjF - G/U mismatch-specific DNA glycosylase (NCBI), at 3,212,989 to 3,213,495
ygjF
b3069: ileX - tRNA-Ile (NCBI), at 3,213,620 to 3,213,695
ileX
b3070: yqjH - predicted siderophore interacting protein (NCBI), at 3,213,749 to 3,214,513
yqjH
b3071: yqjI - predicted transcriptional regulator (NCBI), at 3,214,801 to 3,215,424
yqjI
Position (kb)
3213
3214
3215 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 3213.016 kb on + strand at 3213.058 kb on - strand, within ygjF at 3213.069 kb on - strand, within ygjF at 3213.276 kb on - strand, within ygjF at 3213.334 kb on + strand, within ygjF at 3213.334 kb on + strand, within ygjF at 3213.449 kb on - strand at 3213.497 kb on - strand at 3213.497 kb on - strand at 3213.692 kb on + strand at 3213.789 kb on + strand at 3213.868 kb on - strand, within yqjH at 3213.983 kb on - strand, within yqjH at 3213.995 kb on - strand, within yqjH at 3213.998 kb on - strand, within yqjH at 3213.998 kb on - strand, within yqjH at 3214.144 kb on + strand, within yqjH at 3214.168 kb on - strand, within yqjH at 3214.254 kb on - strand, within yqjH at 3214.289 kb on - strand, within yqjH at 3214.334 kb on - strand, within yqjH at 3214.334 kb on - strand, within yqjH at 3214.347 kb on + strand, within yqjH at 3214.383 kb on - strand, within yqjH at 3214.408 kb on - strand, within yqjH at 3214.440 kb on + strand at 3214.448 kb on - strand at 3214.485 kb on + strand at 3214.511 kb on - strand at 3214.655 kb on + strand at 3214.907 kb on + strand, within yqjI at 3214.923 kb on + strand, within yqjI at 3214.931 kb on - strand, within yqjI at 3215.115 kb on - strand, within yqjI at 3215.115 kb on - strand, within yqjI at 3215.152 kb on + strand, within yqjI at 3215.152 kb on + strand, within yqjI at 3215.224 kb on + strand, within yqjI at 3215.341 kb on - strand, within yqjI at 3215.341 kb on - strand, within yqjI at 3215.343 kb on + strand, within yqjI at 3215.383 kb on + strand at 3215.512 kb on + strand at 3215.513 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HSDP4 remove 3,213,016 + -0.2 3,213,058 - ygjF b3068 0.14 +0.6 3,213,069 - ygjF b3068 0.16 -3.7 3,213,276 - ygjF b3068 0.57 +3.1 3,213,334 + ygjF b3068 0.68 -0.2 3,213,334 + ygjF b3068 0.68 +1.1 3,213,449 - +0.5 3,213,497 - -2.9 3,213,497 - +1.9 3,213,692 + +0.3 3,213,789 + -2.6 3,213,868 - yqjH b3070 0.16 -4.2 3,213,983 - yqjH b3070 0.31 -3.4 3,213,995 - yqjH b3070 0.32 -0.9 3,213,998 - yqjH b3070 0.33 -3.7 3,213,998 - yqjH b3070 0.33 -0.8 3,214,144 + yqjH b3070 0.52 -0.2 3,214,168 - yqjH b3070 0.55 -0.8 3,214,254 - yqjH b3070 0.66 +1.5 3,214,289 - yqjH b3070 0.71 +1.0 3,214,334 - yqjH b3070 0.76 -1.1 3,214,334 - yqjH b3070 0.76 -4.3 3,214,347 + yqjH b3070 0.78 -5.2 3,214,383 - yqjH b3070 0.83 +0.0 3,214,408 - yqjH b3070 0.86 +1.2 3,214,440 + +0.9 3,214,448 - -3.0 3,214,485 + +0.6 3,214,511 - -2.1 3,214,655 + +0.8 3,214,907 + yqjI b3071 0.17 -3.4 3,214,923 + yqjI b3071 0.20 -2.4 3,214,931 - yqjI b3071 0.21 -2.3 3,215,115 - yqjI b3071 0.50 +1.0 3,215,115 - yqjI b3071 0.50 -1.3 3,215,152 + yqjI b3071 0.56 -1.8 3,215,152 + yqjI b3071 0.56 -1.7 3,215,224 + yqjI b3071 0.68 +0.9 3,215,341 - yqjI b3071 0.87 +0.2 3,215,341 - yqjI b3071 0.87 -2.2 3,215,343 + yqjI b3071 0.87 -0.2 3,215,383 + +0.2 3,215,512 + -2.0 3,215,513 - +1.6
Or see this region's nucleotide sequence