Experiment: HSDP4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tar and cheW are separated by 144 nucleotides cheW and cheA are separated by 20 nucleotides cheA and motB are separated by 4 nucleotides motB and motA overlap by 4 nucleotides
b1886: tar - methyl-accepting chemotaxis protein II (NCBI), at 1,969,054 to 1,970,715
tar
b1887: cheW - purine-binding chemotaxis protein (NCBI), at 1,970,860 to 1,971,363
cheW
b1888: cheA - fused chemotactic sensory histidine kinase in two-component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein (NCBI), at 1,971,384 to 1,973,348
cheA
b1889: motB - flagellar motor protein MotB (NCBI), at 1,973,353 to 1,974,279
motB
b1890: motA - flagellar motor protein MotA (NCBI), at 1,974,276 to 1,975,163
motA
Position (kb)
1971
1972
1973
1974 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1970.390 kb on - strand, within tar at 1970.488 kb on - strand, within tar at 1970.489 kb on + strand, within tar at 1970.497 kb on + strand, within tar at 1970.502 kb on + strand, within tar at 1970.602 kb on + strand at 1970.656 kb on - strand at 1970.666 kb on + strand at 1970.676 kb on + strand at 1970.765 kb on - strand at 1970.884 kb on + strand at 1970.989 kb on - strand, within cheW at 1971.037 kb on - strand, within cheW at 1971.075 kb on + strand, within cheW at 1971.116 kb on + strand, within cheW at 1971.129 kb on - strand, within cheW at 1971.140 kb on - strand, within cheW at 1971.165 kb on + strand, within cheW at 1971.165 kb on + strand, within cheW at 1971.173 kb on - strand, within cheW at 1971.247 kb on + strand, within cheW at 1971.247 kb on + strand, within cheW at 1971.250 kb on + strand, within cheW at 1971.251 kb on + strand, within cheW at 1971.251 kb on + strand, within cheW at 1971.272 kb on + strand, within cheW at 1971.275 kb on - strand, within cheW at 1971.539 kb on + strand at 1971.750 kb on + strand, within cheA at 1971.957 kb on - strand, within cheA at 1972.064 kb on + strand, within cheA at 1972.278 kb on - strand, within cheA at 1972.498 kb on + strand, within cheA at 1972.631 kb on + strand, within cheA at 1972.777 kb on + strand, within cheA at 1972.859 kb on + strand, within cheA at 1972.874 kb on + strand, within cheA at 1972.963 kb on + strand, within cheA at 1973.015 kb on - strand, within cheA at 1973.103 kb on - strand, within cheA at 1973.279 kb on - strand at 1973.279 kb on - strand at 1973.443 kb on - strand at 1973.443 kb on - strand at 1973.577 kb on - strand, within motB at 1973.595 kb on - strand, within motB at 1973.697 kb on + strand, within motB at 1973.846 kb on - strand, within motB at 1973.850 kb on - strand, within motB at 1974.089 kb on - strand, within motB at 1974.089 kb on - strand, within motB at 1974.197 kb on + strand at 1974.226 kb on + strand at 1974.233 kb on + strand at 1974.233 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction HSDP4 remove 1,970,390 - tar b1886 0.80 +0.9 1,970,488 - tar b1886 0.86 +1.1 1,970,489 + tar b1886 0.86 +0.8 1,970,497 + tar b1886 0.87 -0.6 1,970,502 + tar b1886 0.87 -1.8 1,970,602 + +1.2 1,970,656 - -1.7 1,970,666 + -4.2 1,970,676 + +0.0 1,970,765 - +1.4 1,970,884 + +0.4 1,970,989 - cheW b1887 0.26 -0.5 1,971,037 - cheW b1887 0.35 +0.4 1,971,075 + cheW b1887 0.43 +0.8 1,971,116 + cheW b1887 0.51 -0.2 1,971,129 - cheW b1887 0.53 +0.4 1,971,140 - cheW b1887 0.56 -4.5 1,971,165 + cheW b1887 0.61 -2.0 1,971,165 + cheW b1887 0.61 -0.9 1,971,173 - cheW b1887 0.62 +2.3 1,971,247 + cheW b1887 0.77 -4.1 1,971,247 + cheW b1887 0.77 -0.6 1,971,250 + cheW b1887 0.77 -0.1 1,971,251 + cheW b1887 0.78 -3.6 1,971,251 + cheW b1887 0.78 +0.6 1,971,272 + cheW b1887 0.82 -4.6 1,971,275 - cheW b1887 0.82 -0.5 1,971,539 + -0.7 1,971,750 + cheA b1888 0.19 +0.1 1,971,957 - cheA b1888 0.29 +0.4 1,972,064 + cheA b1888 0.35 -4.7 1,972,278 - cheA b1888 0.45 +0.2 1,972,498 + cheA b1888 0.57 +0.1 1,972,631 + cheA b1888 0.63 -2.8 1,972,777 + cheA b1888 0.71 +0.4 1,972,859 + cheA b1888 0.75 +0.1 1,972,874 + cheA b1888 0.76 -1.0 1,972,963 + cheA b1888 0.80 +0.8 1,973,015 - cheA b1888 0.83 -3.9 1,973,103 - cheA b1888 0.87 +1.0 1,973,279 - -0.4 1,973,279 - -0.1 1,973,443 - +2.7 1,973,443 - -2.1 1,973,577 - motB b1889 0.24 -4.0 1,973,595 - motB b1889 0.26 +1.3 1,973,697 + motB b1889 0.37 +1.1 1,973,846 - motB b1889 0.53 -0.7 1,973,850 - motB b1889 0.54 +0.3 1,974,089 - motB b1889 0.79 +1.4 1,974,089 - motB b1889 0.79 +0.7 1,974,197 + -0.2 1,974,226 + -0.4 1,974,233 + -2.3 1,974,233 + -2.8
Or see this region's nucleotide sequence