Experiment: RhdML5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yeiW and yeiP are separated by 154 nucleotides yeiP and yeiQ are separated by 222 nucleotides yeiQ and yeiR are separated by 117 nucleotides
b4502: yeiW - hypothetical protein (NCBI), at 2,263,063 to 2,263,317
yeiW
b2171: yeiP - putative elongation factor (VIMSS), at 2,263,472 to 2,264,044
yeiP
b2172: yeiQ - predicted dehydrogenase, NAD-dependent (NCBI), at 2,264,267 to 2,265,733
yeiQ
b2173: yeiR - predicted enzyme (NCBI), at 2,265,851 to 2,266,837
yeiR
Position (kb)
2264
2265
2266 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2263.286 kb on - strand, within yeiW at 2263.321 kb on - strand at 2263.563 kb on + strand, within yeiP at 2263.563 kb on + strand, within yeiP at 2263.575 kb on + strand, within yeiP at 2263.743 kb on - strand, within yeiP at 2263.813 kb on + strand, within yeiP at 2263.826 kb on + strand, within yeiP at 2263.826 kb on + strand, within yeiP at 2263.908 kb on - strand, within yeiP at 2263.929 kb on - strand, within yeiP at 2263.960 kb on - strand, within yeiP at 2264.034 kb on - strand at 2264.039 kb on + strand at 2264.174 kb on - strand at 2264.184 kb on - strand at 2264.228 kb on - strand at 2264.233 kb on + strand at 2264.272 kb on - strand at 2264.316 kb on - strand at 2264.316 kb on - strand at 2264.482 kb on - strand, within yeiQ at 2264.482 kb on - strand, within yeiQ at 2264.497 kb on + strand, within yeiQ at 2264.497 kb on + strand, within yeiQ at 2264.497 kb on + strand, within yeiQ at 2264.505 kb on - strand, within yeiQ at 2264.565 kb on + strand, within yeiQ at 2264.565 kb on + strand, within yeiQ at 2264.672 kb on - strand, within yeiQ at 2264.946 kb on + strand, within yeiQ at 2264.946 kb on + strand, within yeiQ at 2265.000 kb on - strand, within yeiQ at 2265.000 kb on - strand, within yeiQ at 2265.002 kb on - strand, within yeiQ at 2265.069 kb on - strand, within yeiQ at 2265.069 kb on - strand, within yeiQ at 2265.167 kb on + strand, within yeiQ at 2265.167 kb on + strand, within yeiQ at 2265.252 kb on - strand, within yeiQ at 2265.271 kb on + strand, within yeiQ at 2265.379 kb on + strand, within yeiQ at 2265.379 kb on - strand, within yeiQ at 2265.455 kb on + strand, within yeiQ at 2265.455 kb on + strand, within yeiQ at 2265.488 kb on - strand, within yeiQ at 2265.694 kb on - strand at 2265.889 kb on + strand at 2266.013 kb on + strand, within yeiR at 2266.036 kb on + strand, within yeiR at 2266.036 kb on + strand, within yeiR at 2266.075 kb on + strand, within yeiR at 2266.091 kb on - strand, within yeiR at 2266.182 kb on - strand, within yeiR at 2266.182 kb on - strand, within yeiR at 2266.191 kb on - strand, within yeiR at 2266.207 kb on + strand, within yeiR at 2266.223 kb on - strand, within yeiR at 2266.223 kb on - strand, within yeiR at 2266.421 kb on - strand, within yeiR at 2266.421 kb on - strand, within yeiR at 2266.461 kb on - strand, within yeiR at 2266.588 kb on - strand, within yeiR at 2266.651 kb on + strand, within yeiR at 2266.651 kb on + strand, within yeiR at 2266.666 kb on + strand, within yeiR
Per-strain Table
Position Strand Gene LocusTag Fraction RhdML5 remove 2,263,286 - yeiW b4502 0.87 +0.1 2,263,321 - +0.5 2,263,563 + yeiP b2171 0.16 +0.5 2,263,563 + yeiP b2171 0.16 +0.4 2,263,575 + yeiP b2171 0.18 -0.0 2,263,743 - yeiP b2171 0.47 -0.9 2,263,813 + yeiP b2171 0.60 -0.8 2,263,826 + yeiP b2171 0.62 -1.9 2,263,826 + yeiP b2171 0.62 -1.4 2,263,908 - yeiP b2171 0.76 +1.4 2,263,929 - yeiP b2171 0.80 -0.4 2,263,960 - yeiP b2171 0.85 -3.1 2,264,034 - -1.3 2,264,039 + -0.9 2,264,174 - +0.1 2,264,184 - +0.0 2,264,228 - -0.7 2,264,233 + +1.0 2,264,272 - +0.4 2,264,316 - -1.4 2,264,316 - -0.5 2,264,482 - yeiQ b2172 0.15 -0.4 2,264,482 - yeiQ b2172 0.15 +0.9 2,264,497 + yeiQ b2172 0.16 +2.0 2,264,497 + yeiQ b2172 0.16 -1.6 2,264,497 + yeiQ b2172 0.16 +0.5 2,264,505 - yeiQ b2172 0.16 -2.7 2,264,565 + yeiQ b2172 0.20 -1.1 2,264,565 + yeiQ b2172 0.20 -0.5 2,264,672 - yeiQ b2172 0.28 -0.7 2,264,946 + yeiQ b2172 0.46 +0.2 2,264,946 + yeiQ b2172 0.46 +0.7 2,265,000 - yeiQ b2172 0.50 +0.3 2,265,000 - yeiQ b2172 0.50 -2.1 2,265,002 - yeiQ b2172 0.50 -0.2 2,265,069 - yeiQ b2172 0.55 -2.2 2,265,069 - yeiQ b2172 0.55 -1.6 2,265,167 + yeiQ b2172 0.61 -0.1 2,265,167 + yeiQ b2172 0.61 -1.8 2,265,252 - yeiQ b2172 0.67 +0.3 2,265,271 + yeiQ b2172 0.68 -1.6 2,265,379 + yeiQ b2172 0.76 -1.2 2,265,379 - yeiQ b2172 0.76 -0.0 2,265,455 + yeiQ b2172 0.81 -0.8 2,265,455 + yeiQ b2172 0.81 +0.2 2,265,488 - yeiQ b2172 0.83 -0.8 2,265,694 - -0.7 2,265,889 + +0.5 2,266,013 + yeiR b2173 0.16 +0.8 2,266,036 + yeiR b2173 0.19 -0.4 2,266,036 + yeiR b2173 0.19 +1.0 2,266,075 + yeiR b2173 0.23 +0.1 2,266,091 - yeiR b2173 0.24 +0.1 2,266,182 - yeiR b2173 0.34 +1.7 2,266,182 - yeiR b2173 0.34 +1.4 2,266,191 - yeiR b2173 0.34 +0.4 2,266,207 + yeiR b2173 0.36 -0.1 2,266,223 - yeiR b2173 0.38 -1.9 2,266,223 - yeiR b2173 0.38 -0.3 2,266,421 - yeiR b2173 0.58 +0.9 2,266,421 - yeiR b2173 0.58 -0.8 2,266,461 - yeiR b2173 0.62 -1.3 2,266,588 - yeiR b2173 0.75 -0.1 2,266,651 + yeiR b2173 0.81 -0.0 2,266,651 + yeiR b2173 0.81 +0.5 2,266,666 + yeiR b2173 0.83 -0.2
Or see this region's nucleotide sequence