Experiment: Calbicans
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybdK and sokE are separated by 350 nucleotides sokE and hokE are separated by 43 nucleotides hokE and insL-2 are separated by 76 nucleotides
b0581: ybdK - gamma-glutamyl:cysteine ligase (NCBI), at 605,488 to 606,606
ybdK
b4700: sokE - sRNA at remnant mokE/hokE locus (RefSeq), at 606,957 to 607,015
sokE
b4415: hokE - toxic polypeptide, small (NCBI), at 607,059 to 607,211
hokE
b0582: insL-2 - IS186/IS421 transposase (NCBI), at 607,288 to 608,400
insL-2
Position (kb) 607
608 Strain fitness (log2 ratio) -2
-1
0
1 at 606.060 kb on + strand, within ybdK at 606.095 kb on + strand, within ybdK at 606.095 kb on + strand, within ybdK at 606.317 kb on - strand, within ybdK at 606.367 kb on - strand, within ybdK at 606.498 kb on + strand at 606.506 kb on - strand at 606.516 kb on - strand at 606.516 kb on - strand at 606.602 kb on + strand at 606.621 kb on - strand at 606.673 kb on + strand at 606.937 kb on + strand at 606.988 kb on + strand, within sokE at 607.102 kb on - strand, within hokE at 607.105 kb on - strand, within hokE at 607.105 kb on - strand, within hokE at 607.122 kb on + strand, within hokE at 607.185 kb on + strand, within hokE at 607.210 kb on - strand at 607.224 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Calbicans remove 606,060 + ybdK b0581 0.51 +0.4 606,095 + ybdK b0581 0.54 +0.0 606,095 + ybdK b0581 0.54 +0.8 606,317 - ybdK b0581 0.74 -0.7 606,367 - ybdK b0581 0.79 -0.4 606,498 + +0.5 606,506 - -0.2 606,516 - -0.2 606,516 - -0.9 606,602 + +1.2 606,621 - -0.1 606,673 + +1.0 606,937 + -1.3 606,988 + sokE b4700 0.53 -1.8 607,102 - hokE b4415 0.28 -0.2 607,105 - hokE b4415 0.30 -0.0 607,105 - hokE b4415 0.30 -1.1 607,122 + hokE b4415 0.41 -1.7 607,185 + hokE b4415 0.82 -1.2 607,210 - -1.2 607,224 + -0.4
Or see this region's nucleotide sequence