Experiment: Calbicans
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purA and yjeB are separated by 204 nucleotides yjeB and vacB are separated by 38 nucleotides vacB and rlmB are separated by 179 nucleotides
b4177: purA - adenylosuccinate synthetase (NCBI), at 4,402,710 to 4,404,008
purA
b4178: yjeB - predicted DNA-binding transcriptional regulator (NCBI), at 4,404,213 to 4,404,638
yjeB
b4179: vacB - putative enzyme (VIMSS), at 4,404,677 to 4,407,118
vacB
b4180: rlmB - 23S rRNA (Gm2251)-methyltransferase (NCBI), at 4,407,298 to 4,408,029
rlmB
Position (kb)
4404
4405
4406
4407
4408 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4403.855 kb on - strand, within purA at 4403.965 kb on + strand at 4403.967 kb on - strand at 4404.050 kb on + strand at 4404.050 kb on + strand at 4404.050 kb on + strand at 4404.050 kb on + strand at 4404.070 kb on - strand at 4404.095 kb on - strand at 4404.095 kb on - strand at 4404.097 kb on + strand at 4404.097 kb on + strand at 4404.169 kb on - strand at 4404.254 kb on + strand at 4404.311 kb on + strand, within yjeB at 4404.311 kb on + strand, within yjeB at 4404.312 kb on + strand, within yjeB at 4404.312 kb on + strand, within yjeB at 4404.364 kb on - strand, within yjeB at 4404.364 kb on - strand, within yjeB at 4404.379 kb on - strand, within yjeB at 4404.379 kb on - strand, within yjeB at 4404.490 kb on - strand, within yjeB at 4404.536 kb on + strand, within yjeB at 4404.536 kb on + strand, within yjeB at 4404.536 kb on + strand, within yjeB at 4404.536 kb on + strand, within yjeB at 4404.536 kb on + strand, within yjeB at 4404.544 kb on - strand, within yjeB at 4404.544 kb on - strand, within yjeB at 4404.567 kb on + strand, within yjeB at 4404.583 kb on - strand, within yjeB at 4404.600 kb on - strand at 4404.620 kb on - strand at 4404.695 kb on + strand at 4404.695 kb on + strand at 4404.806 kb on - strand at 4404.819 kb on - strand at 4404.873 kb on - strand at 4404.898 kb on + strand at 4404.898 kb on + strand at 4404.898 kb on + strand at 4404.928 kb on + strand, within vacB at 4404.928 kb on + strand, within vacB at 4404.952 kb on + strand, within vacB at 4404.966 kb on + strand, within vacB at 4404.966 kb on + strand, within vacB at 4405.013 kb on - strand, within vacB at 4405.066 kb on - strand, within vacB at 4405.066 kb on - strand, within vacB at 4405.080 kb on - strand, within vacB at 4405.151 kb on - strand, within vacB at 4405.187 kb on + strand, within vacB at 4405.245 kb on + strand, within vacB at 4405.278 kb on - strand, within vacB at 4405.313 kb on + strand, within vacB at 4405.348 kb on + strand, within vacB at 4405.358 kb on - strand, within vacB at 4405.463 kb on - strand, within vacB at 4405.463 kb on - strand, within vacB at 4405.580 kb on + strand, within vacB at 4405.590 kb on + strand, within vacB at 4405.590 kb on + strand, within vacB at 4405.590 kb on + strand, within vacB at 4405.664 kb on + strand, within vacB at 4405.697 kb on - strand, within vacB at 4405.717 kb on + strand, within vacB at 4405.736 kb on + strand, within vacB at 4405.736 kb on + strand, within vacB at 4405.844 kb on + strand, within vacB at 4405.844 kb on + strand, within vacB at 4405.863 kb on + strand, within vacB at 4405.871 kb on - strand, within vacB at 4405.906 kb on + strand, within vacB at 4405.906 kb on + strand, within vacB at 4405.992 kb on + strand, within vacB at 4406.112 kb on + strand, within vacB at 4406.130 kb on - strand, within vacB at 4406.140 kb on + strand, within vacB at 4406.177 kb on - strand, within vacB at 4406.286 kb on - strand, within vacB at 4406.286 kb on - strand, within vacB at 4406.330 kb on - strand, within vacB at 4406.455 kb on + strand, within vacB at 4406.455 kb on + strand, within vacB at 4406.465 kb on + strand, within vacB at 4406.465 kb on + strand, within vacB at 4406.471 kb on - strand, within vacB at 4406.516 kb on - strand, within vacB at 4406.518 kb on + strand, within vacB at 4406.523 kb on + strand, within vacB at 4406.526 kb on - strand, within vacB at 4406.529 kb on - strand, within vacB at 4406.564 kb on + strand, within vacB at 4406.727 kb on - strand, within vacB at 4406.727 kb on - strand, within vacB at 4406.743 kb on - strand, within vacB at 4406.812 kb on + strand, within vacB at 4406.846 kb on - strand, within vacB at 4406.846 kb on - strand, within vacB at 4407.253 kb on + strand at 4407.286 kb on - strand at 4407.328 kb on + strand at 4407.335 kb on + strand at 4407.335 kb on + strand at 4407.336 kb on - strand at 4407.336 kb on - strand at 4407.336 kb on - strand at 4407.336 kb on - strand at 4407.345 kb on - strand at 4407.345 kb on - strand at 4407.346 kb on - strand at 4407.404 kb on + strand, within rlmB at 4407.404 kb on + strand, within rlmB at 4407.501 kb on + strand, within rlmB at 4407.534 kb on - strand, within rlmB at 4407.534 kb on - strand at 4407.704 kb on - strand, within rlmB at 4407.772 kb on - strand, within rlmB at 4407.772 kb on - strand, within rlmB at 4407.776 kb on + strand, within rlmB at 4407.776 kb on + strand, within rlmB at 4407.833 kb on + strand, within rlmB at 4407.842 kb on - strand, within rlmB at 4407.849 kb on - strand, within rlmB at 4407.874 kb on + strand, within rlmB at 4407.876 kb on + strand, within rlmB at 4407.876 kb on + strand, within rlmB at 4407.882 kb on - strand, within rlmB at 4407.889 kb on + strand, within rlmB at 4407.891 kb on - strand, within rlmB at 4407.898 kb on + strand, within rlmB at 4407.906 kb on + strand, within rlmB at 4407.907 kb on + strand, within rlmB at 4407.924 kb on - strand, within rlmB at 4407.941 kb on + strand, within rlmB at 4407.942 kb on - strand, within rlmB at 4407.942 kb on - strand, within rlmB at 4407.951 kb on + strand, within rlmB at 4407.953 kb on - strand, within rlmB at 4407.967 kb on + strand at 4407.967 kb on + strand at 4407.977 kb on + strand at 4408.012 kb on + strand at 4408.012 kb on + strand at 4408.012 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Calbicans remove 4,403,855 - purA b4177 0.88 -0.9 4,403,965 + -0.5 4,403,967 - -0.1 4,404,050 + -0.4 4,404,050 + +0.3 4,404,050 + +0.2 4,404,050 + +0.3 4,404,070 - +0.1 4,404,095 - -0.1 4,404,095 - +0.0 4,404,097 + +1.0 4,404,097 + -2.3 4,404,169 - -0.7 4,404,254 + +0.1 4,404,311 + yjeB b4178 0.23 +0.3 4,404,311 + yjeB b4178 0.23 -0.5 4,404,312 + yjeB b4178 0.23 -2.1 4,404,312 + yjeB b4178 0.23 -0.7 4,404,364 - yjeB b4178 0.35 -0.5 4,404,364 - yjeB b4178 0.35 -1.5 4,404,379 - yjeB b4178 0.39 -2.2 4,404,379 - yjeB b4178 0.39 -0.8 4,404,490 - yjeB b4178 0.65 -1.0 4,404,536 + yjeB b4178 0.76 -2.5 4,404,536 + yjeB b4178 0.76 -1.1 4,404,536 + yjeB b4178 0.76 +0.5 4,404,536 + yjeB b4178 0.76 +1.3 4,404,536 + yjeB b4178 0.76 -0.5 4,404,544 - yjeB b4178 0.78 -1.4 4,404,544 - yjeB b4178 0.78 +0.4 4,404,567 + yjeB b4178 0.83 +0.4 4,404,583 - yjeB b4178 0.87 -1.5 4,404,600 - -0.7 4,404,620 - -0.3 4,404,695 + -0.8 4,404,695 + +0.5 4,404,806 - +0.5 4,404,819 - -2.1 4,404,873 - -1.7 4,404,898 + +0.5 4,404,898 + +0.2 4,404,898 + -0.3 4,404,928 + vacB b4179 0.10 -1.9 4,404,928 + vacB b4179 0.10 -0.0 4,404,952 + vacB b4179 0.11 +0.1 4,404,966 + vacB b4179 0.12 +1.5 4,404,966 + vacB b4179 0.12 -0.8 4,405,013 - vacB b4179 0.14 +0.5 4,405,066 - vacB b4179 0.16 +1.5 4,405,066 - vacB b4179 0.16 +0.5 4,405,080 - vacB b4179 0.17 +2.5 4,405,151 - vacB b4179 0.19 -0.5 4,405,187 + vacB b4179 0.21 -0.1 4,405,245 + vacB b4179 0.23 +1.5 4,405,278 - vacB b4179 0.25 -0.7 4,405,313 + vacB b4179 0.26 -0.1 4,405,348 + vacB b4179 0.27 +0.6 4,405,358 - vacB b4179 0.28 -2.0 4,405,463 - vacB b4179 0.32 -0.5 4,405,463 - vacB b4179 0.32 +0.5 4,405,580 + vacB b4179 0.37 +0.5 4,405,590 + vacB b4179 0.37 +0.0 4,405,590 + vacB b4179 0.37 +1.5 4,405,590 + vacB b4179 0.37 +0.5 4,405,664 + vacB b4179 0.40 +0.5 4,405,697 - vacB b4179 0.42 +1.5 4,405,717 + vacB b4179 0.43 -0.8 4,405,736 + vacB b4179 0.43 -0.1 4,405,736 + vacB b4179 0.43 +0.5 4,405,844 + vacB b4179 0.48 -1.3 4,405,844 + vacB b4179 0.48 -1.1 4,405,863 + vacB b4179 0.49 +1.5 4,405,871 - vacB b4179 0.49 -0.8 4,405,906 + vacB b4179 0.50 +1.9 4,405,906 + vacB b4179 0.50 -1.5 4,405,992 + vacB b4179 0.54 -1.8 4,406,112 + vacB b4179 0.59 +1.5 4,406,130 - vacB b4179 0.60 -0.5 4,406,140 + vacB b4179 0.60 +0.5 4,406,177 - vacB b4179 0.61 -0.1 4,406,286 - vacB b4179 0.66 -1.3 4,406,286 - vacB b4179 0.66 -0.5 4,406,330 - vacB b4179 0.68 -0.1 4,406,455 + vacB b4179 0.73 -1.3 4,406,455 + vacB b4179 0.73 +0.0 4,406,465 + vacB b4179 0.73 +2.1 4,406,465 + vacB b4179 0.73 -0.8 4,406,471 - vacB b4179 0.73 +0.5 4,406,516 - vacB b4179 0.75 +1.5 4,406,518 + vacB b4179 0.75 -0.8 4,406,523 + vacB b4179 0.76 -1.1 4,406,526 - vacB b4179 0.76 +1.5 4,406,529 - vacB b4179 0.76 +1.5 4,406,564 + vacB b4179 0.77 -1.3 4,406,727 - vacB b4179 0.84 -0.8 4,406,727 - vacB b4179 0.84 -0.1 4,406,743 - vacB b4179 0.85 +1.5 4,406,812 + vacB b4179 0.87 -2.3 4,406,846 - vacB b4179 0.89 -1.2 4,406,846 - vacB b4179 0.89 -1.0 4,407,253 + -0.7 4,407,286 - -2.8 4,407,328 + -0.4 4,407,335 + -0.8 4,407,335 + -0.5 4,407,336 - -0.5 4,407,336 - +0.5 4,407,336 - -1.4 4,407,336 - -0.7 4,407,345 - -0.1 4,407,345 - -0.7 4,407,346 - +3.5 4,407,404 + rlmB b4180 0.14 +0.4 4,407,404 + rlmB b4180 0.14 +0.4 4,407,501 + rlmB b4180 0.28 -0.7 4,407,534 - rlmB b4180 0.32 -0.2 4,407,534 - +0.9 4,407,704 - rlmB b4180 0.55 +0.1 4,407,772 - rlmB b4180 0.65 -0.7 4,407,772 - rlmB b4180 0.65 +0.3 4,407,776 + rlmB b4180 0.65 +0.4 4,407,776 + rlmB b4180 0.65 -0.1 4,407,833 + rlmB b4180 0.73 +0.6 4,407,842 - rlmB b4180 0.74 -0.5 4,407,849 - rlmB b4180 0.75 -0.2 4,407,874 + rlmB b4180 0.79 -0.8 4,407,876 + rlmB b4180 0.79 +0.2 4,407,876 + rlmB b4180 0.79 +2.1 4,407,882 - rlmB b4180 0.80 -1.7 4,407,889 + rlmB b4180 0.81 -1.2 4,407,891 - rlmB b4180 0.81 -0.2 4,407,898 + rlmB b4180 0.82 -0.5 4,407,906 + rlmB b4180 0.83 -1.2 4,407,907 + rlmB b4180 0.83 -0.1 4,407,924 - rlmB b4180 0.86 -0.8 4,407,941 + rlmB b4180 0.88 +0.2 4,407,942 - rlmB b4180 0.88 -0.1 4,407,942 - rlmB b4180 0.88 +1.9 4,407,951 + rlmB b4180 0.89 +1.2 4,407,953 - rlmB b4180 0.89 -1.1 4,407,967 + -1.5 4,407,967 + -0.4 4,407,977 + -0.2 4,408,012 + -2.2 4,408,012 + +0.2 4,408,012 - +1.5
Or see this region's nucleotide sequence