Experiment: Calbicans
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dusC and yohJ are separated by 238 nucleotides yohJ and yohK overlap by 4 nucleotides yohK and cdd are separated by 129 nucleotides
b2140: dusC - tRNA-dihydrouridine synthase C (NCBI), at 2,227,460 to 2,228,407
dusC
b2141: yohJ - hypothetical protein (NCBI), at 2,228,646 to 2,229,044
yohJ
b2142: yohK - predicted inner membrane protein (NCBI), at 2,229,041 to 2,229,736
yohK
b2143: cdd - cytidine deaminase (NCBI), at 2,229,866 to 2,230,750
cdd
Position (kb)
2229
2230 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2228.138 kb on + strand, within dusC at 2228.327 kb on - strand at 2228.444 kb on - strand at 2228.444 kb on - strand at 2228.446 kb on - strand at 2228.446 kb on - strand at 2228.530 kb on - strand at 2228.542 kb on - strand at 2228.542 kb on - strand at 2228.637 kb on - strand at 2228.637 kb on - strand at 2228.716 kb on - strand, within yohJ at 2228.716 kb on - strand, within yohJ at 2228.753 kb on + strand, within yohJ at 2228.753 kb on + strand, within yohJ at 2228.951 kb on + strand, within yohJ at 2229.015 kb on + strand at 2229.068 kb on - strand at 2229.123 kb on - strand, within yohK at 2229.123 kb on - strand, within yohK at 2229.123 kb on - strand, within yohK at 2229.123 kb on - strand, within yohK at 2229.190 kb on + strand, within yohK at 2229.221 kb on + strand, within yohK at 2229.221 kb on + strand, within yohK at 2229.259 kb on + strand, within yohK at 2229.272 kb on - strand, within yohK at 2229.279 kb on + strand, within yohK at 2229.279 kb on + strand, within yohK at 2229.284 kb on + strand, within yohK at 2229.284 kb on + strand, within yohK at 2229.292 kb on - strand, within yohK at 2229.525 kb on + strand, within yohK at 2229.580 kb on + strand, within yohK at 2229.588 kb on - strand, within yohK at 2229.672 kb on - strand at 2229.728 kb on - strand at 2229.728 kb on - strand at 2229.972 kb on - strand, within cdd at 2229.972 kb on - strand, within cdd at 2229.984 kb on - strand, within cdd at 2230.246 kb on + strand, within cdd at 2230.255 kb on - strand, within cdd at 2230.398 kb on - strand, within cdd at 2230.398 kb on - strand, within cdd at 2230.624 kb on - strand, within cdd at 2230.685 kb on + strand at 2230.685 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Calbicans remove 2,228,138 + dusC b2140 0.72 +0.6 2,228,327 - -0.4 2,228,444 - -0.1 2,228,444 - -0.7 2,228,446 - -0.2 2,228,446 - -1.0 2,228,530 - -0.1 2,228,542 - +0.1 2,228,542 - -1.1 2,228,637 - -0.2 2,228,637 - -0.6 2,228,716 - yohJ b2141 0.18 +0.5 2,228,716 - yohJ b2141 0.18 +0.3 2,228,753 + yohJ b2141 0.27 -0.4 2,228,753 + yohJ b2141 0.27 -0.7 2,228,951 + yohJ b2141 0.76 -0.7 2,229,015 + -0.0 2,229,068 - +1.6 2,229,123 - yohK b2142 0.12 -1.3 2,229,123 - yohK b2142 0.12 -0.1 2,229,123 - yohK b2142 0.12 -0.9 2,229,123 - yohK b2142 0.12 -0.8 2,229,190 + yohK b2142 0.21 -1.1 2,229,221 + yohK b2142 0.26 -0.4 2,229,221 + yohK b2142 0.26 -0.9 2,229,259 + yohK b2142 0.31 +0.0 2,229,272 - yohK b2142 0.33 -0.6 2,229,279 + yohK b2142 0.34 -1.8 2,229,279 + yohK b2142 0.34 -2.2 2,229,284 + yohK b2142 0.35 +1.1 2,229,284 + yohK b2142 0.35 +0.4 2,229,292 - yohK b2142 0.36 -2.1 2,229,525 + yohK b2142 0.70 -0.1 2,229,580 + yohK b2142 0.77 -1.6 2,229,588 - yohK b2142 0.79 -1.5 2,229,672 - -0.4 2,229,728 - +0.1 2,229,728 - +0.4 2,229,972 - cdd b2143 0.12 -1.6 2,229,972 - cdd b2143 0.12 +0.1 2,229,984 - cdd b2143 0.13 +0.1 2,230,246 + cdd b2143 0.43 -0.7 2,230,255 - cdd b2143 0.44 -0.7 2,230,398 - cdd b2143 0.60 +1.4 2,230,398 - cdd b2143 0.60 +2.8 2,230,624 - cdd b2143 0.86 -1.2 2,230,685 + +2.3 2,230,685 + -0.2
Or see this region's nucleotide sequence