Experiment: Calbicans
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliK and fliL are separated by 104 nucleotides fliL and fliM are separated by 4 nucleotides
b1943: fliK - flagellar hook-length control protein (NCBI), at 2,016,410 to 2,017,537
fliK
b1944: fliL - flagellar basal body-associated protein FliL (NCBI), at 2,017,642 to 2,018,106
fliL
b1945: fliM - flagellar motor switch protein M (NCBI), at 2,018,111 to 2,019,115
fliM
Position (kb)
2017
2018
2019 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2016.658 kb on - strand, within fliK at 2016.927 kb on - strand, within fliK at 2016.957 kb on - strand, within fliK at 2017.027 kb on + strand, within fliK at 2017.068 kb on - strand, within fliK at 2017.076 kb on + strand, within fliK at 2017.076 kb on + strand, within fliK at 2017.080 kb on - strand, within fliK at 2017.116 kb on + strand, within fliK at 2017.182 kb on + strand, within fliK at 2017.207 kb on + strand, within fliK at 2017.207 kb on + strand, within fliK at 2017.267 kb on + strand, within fliK at 2017.323 kb on + strand, within fliK at 2017.488 kb on + strand at 2017.757 kb on + strand, within fliL at 2017.757 kb on + strand, within fliL at 2017.797 kb on - strand, within fliL at 2017.813 kb on + strand, within fliL at 2017.947 kb on - strand, within fliL at 2017.972 kb on + strand, within fliL at 2018.029 kb on - strand, within fliL at 2018.038 kb on - strand, within fliL at 2018.038 kb on - strand, within fliL at 2018.299 kb on + strand, within fliM at 2018.411 kb on + strand, within fliM at 2018.420 kb on - strand, within fliM at 2018.466 kb on + strand, within fliM at 2018.466 kb on + strand, within fliM at 2018.608 kb on - strand, within fliM at 2018.608 kb on - strand, within fliM at 2018.610 kb on - strand, within fliM at 2018.610 kb on - strand, within fliM at 2018.652 kb on - strand, within fliM at 2018.689 kb on - strand, within fliM at 2018.689 kb on - strand, within fliM at 2018.700 kb on + strand, within fliM at 2018.870 kb on + strand, within fliM at 2018.875 kb on + strand, within fliM at 2018.890 kb on - strand, within fliM at 2019.025 kb on + strand at 2019.106 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Calbicans remove 2,016,658 - fliK b1943 0.22 +0.4 2,016,927 - fliK b1943 0.46 -0.8 2,016,957 - fliK b1943 0.48 -0.9 2,017,027 + fliK b1943 0.55 +0.3 2,017,068 - fliK b1943 0.58 -0.6 2,017,076 + fliK b1943 0.59 -0.7 2,017,076 + fliK b1943 0.59 -0.3 2,017,080 - fliK b1943 0.59 -0.1 2,017,116 + fliK b1943 0.63 -0.2 2,017,182 + fliK b1943 0.68 +0.1 2,017,207 + fliK b1943 0.71 +2.1 2,017,207 + fliK b1943 0.71 +0.6 2,017,267 + fliK b1943 0.76 -0.6 2,017,323 + fliK b1943 0.81 -0.8 2,017,488 + -0.4 2,017,757 + fliL b1944 0.25 +0.2 2,017,757 + fliL b1944 0.25 -1.4 2,017,797 - fliL b1944 0.33 -1.0 2,017,813 + fliL b1944 0.37 -1.5 2,017,947 - fliL b1944 0.66 -0.4 2,017,972 + fliL b1944 0.71 -0.2 2,018,029 - fliL b1944 0.83 -0.1 2,018,038 - fliL b1944 0.85 -1.2 2,018,038 - fliL b1944 0.85 -1.4 2,018,299 + fliM b1945 0.19 -0.1 2,018,411 + fliM b1945 0.30 +1.1 2,018,420 - fliM b1945 0.31 +0.3 2,018,466 + fliM b1945 0.35 +0.4 2,018,466 + fliM b1945 0.35 +0.1 2,018,608 - fliM b1945 0.49 +0.9 2,018,608 - fliM b1945 0.49 +1.5 2,018,610 - fliM b1945 0.50 +1.2 2,018,610 - fliM b1945 0.50 +0.8 2,018,652 - fliM b1945 0.54 -0.4 2,018,689 - fliM b1945 0.58 +1.7 2,018,689 - fliM b1945 0.58 -0.9 2,018,700 + fliM b1945 0.59 -1.3 2,018,870 + fliM b1945 0.76 -0.2 2,018,875 + fliM b1945 0.76 +0.4 2,018,890 - fliM b1945 0.78 -0.0 2,019,025 + -1.9 2,019,106 + +0.0
Or see this region's nucleotide sequence