Experiment: Calbicans
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt chbC and chbB are separated by 84 nucleotides chbB and osmE are separated by 298 nucleotides osmE and nadE are separated by 201 nucleotides
b1737: chbC - N,N'-diacetylchitobiose-specific enzyme IIC component of PTS (NCBI), at 1,817,880 to 1,819,238
chbC
b1738: chbB - N,N'-diacetylchitobiose-specific enzyme IIB component of PTS (NCBI), at 1,819,323 to 1,819,643
chbB
b1739: osmE - DNA-binding transcriptional activator (NCBI), at 1,819,942 to 1,820,280
osmE
b1740: nadE - NAD synthetase (NCBI), at 1,820,482 to 1,821,309
nadE
Position (kb)
1819
1820
1821 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1818.975 kb on + strand, within chbC at 1819.023 kb on + strand, within chbC at 1819.024 kb on + strand, within chbC at 1819.115 kb on + strand at 1819.130 kb on - strand at 1819.130 kb on - strand at 1819.159 kb on + strand at 1819.368 kb on - strand, within chbB at 1819.368 kb on - strand, within chbB at 1819.368 kb on - strand, within chbB at 1819.368 kb on - strand, within chbB at 1819.368 kb on - strand, within chbB at 1819.370 kb on - strand, within chbB at 1819.378 kb on - strand, within chbB at 1819.378 kb on - strand, within chbB at 1819.378 kb on - strand, within chbB at 1819.379 kb on - strand, within chbB at 1819.442 kb on + strand, within chbB at 1819.595 kb on - strand, within chbB at 1819.595 kb on - strand, within chbB at 1819.709 kb on - strand at 1819.711 kb on - strand at 1819.711 kb on - strand at 1819.756 kb on - strand at 1819.758 kb on + strand at 1819.761 kb on - strand at 1819.773 kb on - strand at 1819.773 kb on - strand at 1819.773 kb on - strand at 1819.959 kb on + strand at 1819.981 kb on + strand, within osmE at 1819.996 kb on + strand, within osmE at 1820.033 kb on - strand, within osmE at 1820.215 kb on - strand, within osmE
Per-strain Table
Position Strand Gene LocusTag Fraction Calbicans remove 1,818,975 + chbC b1737 0.81 +1.5 1,819,023 + chbC b1737 0.84 -0.7 1,819,024 + chbC b1737 0.84 +1.5 1,819,115 + +0.2 1,819,130 - +1.5 1,819,130 - +1.5 1,819,159 + -1.5 1,819,368 - chbB b1738 0.14 -0.8 1,819,368 - chbB b1738 0.14 +1.5 1,819,368 - chbB b1738 0.14 -0.2 1,819,368 - chbB b1738 0.14 -0.0 1,819,368 - chbB b1738 0.14 -3.2 1,819,370 - chbB b1738 0.15 +0.5 1,819,378 - chbB b1738 0.17 -0.3 1,819,378 - chbB b1738 0.17 -3.6 1,819,378 - chbB b1738 0.17 +0.2 1,819,379 - chbB b1738 0.17 -1.0 1,819,442 + chbB b1738 0.37 +1.5 1,819,595 - chbB b1738 0.85 -0.4 1,819,595 - chbB b1738 0.85 -1.7 1,819,709 - +0.1 1,819,711 - -1.7 1,819,711 - +0.4 1,819,756 - +0.1 1,819,758 + -0.7 1,819,761 - -2.9 1,819,773 - -1.1 1,819,773 - -0.5 1,819,773 - +1.5 1,819,959 + +1.5 1,819,981 + osmE b1739 0.12 -0.1 1,819,996 + osmE b1739 0.16 -1.1 1,820,033 - osmE b1739 0.27 -1.7 1,820,215 - osmE b1739 0.81 -0.7
Or see this region's nucleotide sequence