Experiment: Calbicans
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydjN and ydjO are separated by 3 nucleotides ydjO and cedA are separated by 288 nucleotides cedA and katE are separated by 203 nucleotides
b1729: ydjN - predicted transporter (NCBI), at 1,808,958 to 1,810,349
ydjN
b1730: ydjO - predicted protein (RefSeq), at 1,810,353 to 1,811,156
ydjO
b1731: cedA - cell division modulator (RefSeq), at 1,811,445 to 1,811,687
cedA
b1732: katE - hydroperoxidase HPII(III) (catalase) (NCBI), at 1,811,891 to 1,814,152
katE
Position (kb)
1810
1811
1812 Strain fitness (log2 ratio)
-2
-1
0
1 at 1809.720 kb on + strand, within ydjN at 1809.720 kb on + strand, within ydjN at 1810.253 kb on + strand at 1810.253 kb on + strand at 1810.284 kb on + strand at 1810.284 kb on + strand at 1810.343 kb on + strand at 1810.445 kb on - strand, within ydjO at 1810.493 kb on + strand, within ydjO at 1810.515 kb on + strand, within ydjO at 1811.304 kb on - strand at 1811.393 kb on - strand at 1811.444 kb on - strand at 1811.505 kb on - strand, within cedA at 1811.536 kb on - strand, within cedA at 1811.536 kb on - strand, within cedA at 1811.541 kb on + strand, within cedA at 1811.814 kb on + strand at 1811.821 kb on + strand at 1812.027 kb on + strand at 1812.052 kb on + strand at 1812.060 kb on - strand at 1812.106 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Calbicans remove 1,809,720 + ydjN b1729 0.55 -0.2 1,809,720 + ydjN b1729 0.55 +0.1 1,810,253 + -1.3 1,810,253 + -1.1 1,810,284 + +0.4 1,810,284 + -0.1 1,810,343 + -0.2 1,810,445 - ydjO b1730 0.11 +1.5 1,810,493 + ydjO b1730 0.17 -2.1 1,810,515 + ydjO b1730 0.20 +1.5 1,811,304 - +1.5 1,811,393 - -0.1 1,811,444 - -0.1 1,811,505 - cedA b1731 0.25 -0.1 1,811,536 - cedA b1731 0.37 -0.3 1,811,536 - cedA b1731 0.37 -1.1 1,811,541 + cedA b1731 0.40 -0.5 1,811,814 + -0.3 1,811,821 + +0.7 1,812,027 + +0.7 1,812,052 + +0.6 1,812,060 - +0.6 1,812,106 - +0.2
Or see this region's nucleotide sequence