Experiment: Calbicans
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydhY and ydhZ are separated by 454 nucleotides ydhZ and pykF are separated by 556 nucleotides
b1674: ydhY - predicted 4Fe-4S ferridoxin-type protein (NCBI), at 1,751,875 to 1,752,501
ydhY
b1675: ydhZ - hypothetical protein (NCBI), at 1,752,956 to 1,753,165
ydhZ
b1676: pykF - pyruvate kinase (NCBI), at 1,753,722 to 1,755,134
pykF
Position (kb)
1752
1753
1754 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1751.957 kb on - strand, within ydhY at 1752.011 kb on + strand, within ydhY at 1752.012 kb on - strand, within ydhY at 1752.017 kb on - strand, within ydhY at 1752.022 kb on - strand, within ydhY at 1752.022 kb on - strand, within ydhY at 1752.035 kb on - strand, within ydhY at 1752.120 kb on - strand, within ydhY at 1752.237 kb on - strand, within ydhY at 1752.261 kb on + strand, within ydhY at 1752.262 kb on - strand, within ydhY at 1752.262 kb on - strand, within ydhY at 1752.262 kb on - strand, within ydhY at 1752.262 kb on - strand, within ydhY at 1752.291 kb on + strand, within ydhY at 1752.316 kb on - strand, within ydhY at 1752.319 kb on + strand, within ydhY at 1752.377 kb on - strand, within ydhY at 1752.410 kb on + strand, within ydhY at 1752.411 kb on + strand, within ydhY at 1752.455 kb on + strand at 1752.532 kb on - strand at 1752.539 kb on - strand at 1752.546 kb on - strand at 1752.566 kb on - strand at 1752.572 kb on - strand at 1752.574 kb on - strand at 1752.574 kb on - strand at 1752.593 kb on - strand at 1752.920 kb on - strand at 1752.934 kb on - strand at 1753.041 kb on - strand, within ydhZ at 1753.125 kb on - strand, within ydhZ at 1753.126 kb on - strand, within ydhZ at 1753.141 kb on + strand, within ydhZ at 1753.300 kb on - strand at 1753.300 kb on - strand at 1753.313 kb on + strand at 1753.334 kb on + strand at 1753.334 kb on + strand at 1753.334 kb on + strand at 1753.342 kb on - strand at 1753.342 kb on - strand at 1753.342 kb on - strand at 1753.358 kb on + strand at 1753.500 kb on - strand at 1753.500 kb on - strand at 1753.563 kb on + strand at 1753.582 kb on + strand at 1753.615 kb on + strand at 1753.635 kb on - strand at 1753.696 kb on + strand at 1753.710 kb on - strand at 1753.710 kb on - strand at 1753.836 kb on - strand at 1753.836 kb on - strand at 1753.921 kb on + strand, within pykF at 1753.983 kb on + strand, within pykF at 1754.072 kb on + strand, within pykF at 1754.121 kb on - strand, within pykF at 1754.121 kb on - strand, within pykF
Per-strain Table
Position Strand Gene LocusTag Fraction Calbicans remove 1,751,957 - ydhY b1674 0.13 -0.4 1,752,011 + ydhY b1674 0.22 +1.5 1,752,012 - ydhY b1674 0.22 +0.4 1,752,017 - ydhY b1674 0.23 +0.5 1,752,022 - ydhY b1674 0.23 +1.0 1,752,022 - ydhY b1674 0.23 +0.6 1,752,035 - ydhY b1674 0.26 -0.4 1,752,120 - ydhY b1674 0.39 -0.1 1,752,237 - ydhY b1674 0.58 +1.9 1,752,261 + ydhY b1674 0.62 -0.9 1,752,262 - ydhY b1674 0.62 +1.1 1,752,262 - ydhY b1674 0.62 +0.5 1,752,262 - ydhY b1674 0.62 -0.5 1,752,262 - ydhY b1674 0.62 -2.0 1,752,291 + ydhY b1674 0.66 -0.7 1,752,316 - ydhY b1674 0.70 +0.5 1,752,319 + ydhY b1674 0.71 +1.5 1,752,377 - ydhY b1674 0.80 -2.5 1,752,410 + ydhY b1674 0.85 -0.9 1,752,411 + ydhY b1674 0.85 -0.1 1,752,455 + +1.5 1,752,532 - -1.3 1,752,539 - -0.9 1,752,546 - +1.5 1,752,566 - -0.1 1,752,572 - +2.5 1,752,574 - -0.1 1,752,574 - +1.5 1,752,593 - -0.1 1,752,920 - +0.5 1,752,934 - +0.5 1,753,041 - ydhZ b1675 0.40 -0.4 1,753,125 - ydhZ b1675 0.80 -0.7 1,753,126 - ydhZ b1675 0.81 -1.2 1,753,141 + ydhZ b1675 0.88 +1.5 1,753,300 - -0.5 1,753,300 - +0.5 1,753,313 + +1.5 1,753,334 + -0.5 1,753,334 + +3.5 1,753,334 + +0.7 1,753,342 - -1.4 1,753,342 - -0.3 1,753,342 - +1.0 1,753,358 + +2.1 1,753,500 - -1.5 1,753,500 - +1.5 1,753,563 + -2.0 1,753,582 + +0.9 1,753,615 + -0.1 1,753,635 - -0.5 1,753,696 + -0.4 1,753,710 - +1.5 1,753,710 - -0.1 1,753,836 - -0.3 1,753,836 - +2.1 1,753,921 + pykF b1676 0.14 -0.4 1,753,983 + pykF b1676 0.18 +0.5 1,754,072 + pykF b1676 0.25 -1.7 1,754,121 - pykF b1676 0.28 -0.1 1,754,121 - pykF b1676 0.28 +0.6
Or see this region's nucleotide sequence