Experiment: Calbicans
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pntB and pntA are separated by 10 nucleotides pntA and ydgH are separated by 523 nucleotides
b1602: pntB - pyridine nucleotide transhydrogenase (NCBI), at 1,672,996 to 1,674,384
pntB
b1603: pntA - NAD(P) transhydrogenase subunit alpha (NCBI), at 1,674,395 to 1,675,927
pntA
b1604: ydgH - hypothetical protein (NCBI), at 1,676,451 to 1,677,395
ydgH
Position (kb)
1674
1675
1676 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1673.501 kb on - strand, within pntB at 1673.579 kb on - strand, within pntB at 1673.579 kb on - strand, within pntB at 1673.667 kb on + strand, within pntB at 1673.672 kb on + strand, within pntB at 1673.672 kb on + strand, within pntB at 1673.878 kb on + strand, within pntB at 1673.878 kb on + strand, within pntB at 1674.216 kb on + strand, within pntB at 1674.370 kb on - strand at 1674.385 kb on + strand at 1674.511 kb on - strand at 1674.521 kb on - strand at 1674.521 kb on - strand at 1674.523 kb on + strand at 1674.583 kb on - strand, within pntA at 1674.865 kb on + strand, within pntA at 1674.894 kb on - strand, within pntA at 1675.064 kb on + strand, within pntA at 1675.064 kb on + strand, within pntA at 1675.987 kb on + strand at 1675.987 kb on + strand at 1675.997 kb on - strand at 1675.997 kb on - strand at 1676.126 kb on - strand at 1676.139 kb on - strand at 1676.139 kb on - strand at 1676.139 kb on - strand at 1676.139 kb on - strand at 1676.154 kb on - strand at 1676.257 kb on - strand at 1676.257 kb on - strand at 1676.418 kb on + strand at 1676.523 kb on + strand at 1676.770 kb on + strand, within ydgH at 1676.818 kb on - strand, within ydgH
Per-strain Table
Position Strand Gene LocusTag Fraction Calbicans remove 1,673,501 - pntB b1602 0.36 +0.3 1,673,579 - pntB b1602 0.42 -0.5 1,673,579 - pntB b1602 0.42 -0.3 1,673,667 + pntB b1602 0.48 -1.3 1,673,672 + pntB b1602 0.49 +0.4 1,673,672 + pntB b1602 0.49 +0.7 1,673,878 + pntB b1602 0.63 -0.1 1,673,878 + pntB b1602 0.63 +0.5 1,674,216 + pntB b1602 0.88 -1.4 1,674,370 - +0.4 1,674,385 + +0.1 1,674,511 - -0.2 1,674,521 - +2.7 1,674,521 - -0.3 1,674,523 + +0.9 1,674,583 - pntA b1603 0.12 -0.4 1,674,865 + pntA b1603 0.31 -1.3 1,674,894 - pntA b1603 0.33 +0.5 1,675,064 + pntA b1603 0.44 -3.0 1,675,064 + pntA b1603 0.44 -1.4 1,675,987 + +0.0 1,675,987 + +0.2 1,675,997 - -1.4 1,675,997 - -0.4 1,676,126 - +0.9 1,676,139 - -0.7 1,676,139 - +0.7 1,676,139 - -0.9 1,676,139 - +0.5 1,676,154 - +0.0 1,676,257 - +0.4 1,676,257 - +0.1 1,676,418 + +0.4 1,676,523 + -2.0 1,676,770 + ydgH b1604 0.34 +1.9 1,676,818 - ydgH b1604 0.39 -0.1
Or see this region's nucleotide sequence