Experiment: Calbicans
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yegX and thiD are separated by 64 nucleotides thiD and thiM overlap by 4 nucleotides thiM and yohL are separated by 222 nucleotides yohL and yohM are separated by 120 nucleotides
b2102: yegX - orf, hypothetical protein (VIMSS), at 2,180,855 to 2,181,673
yegX
b2103: thiD - phosphomethylpyrimidine kinase (NCBI), at 2,181,738 to 2,182,538
thiD
b2104: thiM - hydroxyethylthiazole kinase (NCBI), at 2,182,535 to 2,183,323
thiM
b2105: yohL - hypothetical protein (NCBI), at 2,183,546 to 2,183,818
yohL
b2106: yohM - membrane protein conferring nickel and cobalt resistance (NCBI), at 2,183,939 to 2,184,763
yohM
Position (kb)
2182
2183
2184 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2181.548 kb on + strand, within yegX at 2181.628 kb on + strand at 2181.628 kb on + strand at 2181.631 kb on + strand at 2181.660 kb on + strand at 2181.678 kb on - strand at 2181.695 kb on + strand at 2181.726 kb on + strand at 2181.731 kb on - strand at 2181.749 kb on - strand at 2181.801 kb on - strand at 2181.806 kb on - strand at 2181.875 kb on + strand, within thiD at 2181.883 kb on - strand, within thiD at 2181.883 kb on - strand, within thiD at 2181.883 kb on - strand, within thiD at 2182.009 kb on + strand, within thiD at 2182.009 kb on + strand, within thiD at 2182.012 kb on + strand, within thiD at 2182.020 kb on - strand, within thiD at 2182.119 kb on - strand, within thiD at 2182.150 kb on - strand, within thiD at 2182.161 kb on + strand, within thiD at 2182.375 kb on + strand, within thiD at 2182.375 kb on + strand, within thiD at 2182.382 kb on + strand, within thiD at 2182.546 kb on - strand at 2182.646 kb on + strand, within thiM at 2183.006 kb on - strand, within thiM at 2183.006 kb on - strand, within thiM at 2183.044 kb on + strand, within thiM at 2183.246 kb on + strand at 2183.246 kb on + strand at 2183.259 kb on - strand at 2183.259 kb on - strand at 2183.389 kb on - strand at 2183.399 kb on - strand at 2183.399 kb on - strand at 2183.399 kb on - strand at 2183.495 kb on - strand at 2183.533 kb on - strand at 2183.614 kb on + strand, within yohL at 2183.614 kb on - strand, within yohL at 2183.620 kb on - strand, within yohL at 2183.621 kb on + strand, within yohL at 2183.766 kb on - strand, within yohL at 2183.814 kb on - strand at 2183.814 kb on - strand at 2183.989 kb on + strand at 2184.011 kb on + strand at 2184.014 kb on + strand at 2184.014 kb on + strand at 2184.030 kb on - strand, within yohM at 2184.141 kb on + strand, within yohM at 2184.141 kb on + strand, within yohM at 2184.272 kb on + strand, within yohM
Per-strain Table
Position Strand Gene LocusTag Fraction Calbicans remove 2,181,548 + yegX b2102 0.85 -0.0 2,181,628 + -0.4 2,181,628 + -1.1 2,181,631 + -0.6 2,181,660 + +0.5 2,181,678 - -0.6 2,181,695 + -0.3 2,181,726 + -1.8 2,181,731 - -0.1 2,181,749 - -0.3 2,181,801 - -0.5 2,181,806 - -0.0 2,181,875 + thiD b2103 0.17 -0.2 2,181,883 - thiD b2103 0.18 +2.0 2,181,883 - thiD b2103 0.18 -0.1 2,181,883 - thiD b2103 0.18 -1.4 2,182,009 + thiD b2103 0.34 +1.1 2,182,009 + thiD b2103 0.34 -1.5 2,182,012 + thiD b2103 0.34 -0.7 2,182,020 - thiD b2103 0.35 -0.5 2,182,119 - thiD b2103 0.48 -0.3 2,182,150 - thiD b2103 0.51 -0.3 2,182,161 + thiD b2103 0.53 -0.0 2,182,375 + thiD b2103 0.80 -0.3 2,182,375 + thiD b2103 0.80 -1.8 2,182,382 + thiD b2103 0.80 +0.5 2,182,546 - -0.1 2,182,646 + thiM b2104 0.14 -0.5 2,183,006 - thiM b2104 0.60 -1.5 2,183,006 - thiM b2104 0.60 +0.2 2,183,044 + thiM b2104 0.65 -0.4 2,183,246 + +0.1 2,183,246 + +1.4 2,183,259 - +0.3 2,183,259 - +0.5 2,183,389 - -0.8 2,183,399 - -1.9 2,183,399 - -0.7 2,183,399 - +0.3 2,183,495 - +0.4 2,183,533 - +0.5 2,183,614 + yohL b2105 0.25 -1.8 2,183,614 - yohL b2105 0.25 -0.6 2,183,620 - yohL b2105 0.27 -0.1 2,183,621 + yohL b2105 0.27 +0.5 2,183,766 - yohL b2105 0.81 +0.1 2,183,814 - -1.7 2,183,814 - -1.2 2,183,989 + -0.4 2,184,011 + -0.1 2,184,014 + +0.3 2,184,014 + -1.0 2,184,030 - yohM b2106 0.11 -0.9 2,184,141 + yohM b2106 0.24 +0.4 2,184,141 + yohM b2106 0.24 +0.9 2,184,272 + yohM b2106 0.40 -2.8
Or see this region's nucleotide sequence