Strain Fitness in Escherichia coli BW25113 around b2104

Experiment: Calbicans

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyegX and thiD are separated by 64 nucleotidesthiD and thiM overlap by 4 nucleotidesthiM and yohL are separated by 222 nucleotidesyohL and yohM are separated by 120 nucleotides b2102: yegX - orf, hypothetical protein (VIMSS), at 2,180,855 to 2,181,673 yegX b2103: thiD - phosphomethylpyrimidine kinase (NCBI), at 2,181,738 to 2,182,538 thiD b2104: thiM - hydroxyethylthiazole kinase (NCBI), at 2,182,535 to 2,183,323 thiM b2105: yohL - hypothetical protein (NCBI), at 2,183,546 to 2,183,818 yohL b2106: yohM - membrane protein conferring nickel and cobalt resistance (NCBI), at 2,183,939 to 2,184,763 yohM Position (kb) 2182 2183 2184Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2181.548 kb on + strand, within yegXat 2181.628 kb on + strandat 2181.628 kb on + strandat 2181.631 kb on + strandat 2181.660 kb on + strandat 2181.678 kb on - strandat 2181.695 kb on + strandat 2181.726 kb on + strandat 2181.731 kb on - strandat 2181.749 kb on - strandat 2181.801 kb on - strandat 2181.806 kb on - strandat 2181.875 kb on + strand, within thiDat 2181.883 kb on - strand, within thiDat 2181.883 kb on - strand, within thiDat 2181.883 kb on - strand, within thiDat 2182.009 kb on + strand, within thiDat 2182.009 kb on + strand, within thiDat 2182.012 kb on + strand, within thiDat 2182.020 kb on - strand, within thiDat 2182.119 kb on - strand, within thiDat 2182.150 kb on - strand, within thiDat 2182.161 kb on + strand, within thiDat 2182.375 kb on + strand, within thiDat 2182.375 kb on + strand, within thiDat 2182.382 kb on + strand, within thiDat 2182.546 kb on - strandat 2182.646 kb on + strand, within thiMat 2183.006 kb on - strand, within thiMat 2183.006 kb on - strand, within thiMat 2183.044 kb on + strand, within thiMat 2183.246 kb on + strandat 2183.246 kb on + strandat 2183.259 kb on - strandat 2183.259 kb on - strandat 2183.389 kb on - strandat 2183.399 kb on - strandat 2183.399 kb on - strandat 2183.399 kb on - strandat 2183.495 kb on - strandat 2183.533 kb on - strandat 2183.614 kb on + strand, within yohLat 2183.614 kb on - strand, within yohLat 2183.620 kb on - strand, within yohLat 2183.621 kb on + strand, within yohLat 2183.766 kb on - strand, within yohLat 2183.814 kb on - strandat 2183.814 kb on - strandat 2183.989 kb on + strandat 2184.011 kb on + strandat 2184.014 kb on + strandat 2184.014 kb on + strandat 2184.030 kb on - strand, within yohMat 2184.141 kb on + strand, within yohMat 2184.141 kb on + strand, within yohMat 2184.272 kb on + strand, within yohM

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Per-strain Table

Position Strand Gene LocusTag Fraction Calbicans
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2,181,548 + yegX b2102 0.85 -0.0
2,181,628 + -0.4
2,181,628 + -1.1
2,181,631 + -0.6
2,181,660 + +0.5
2,181,678 - -0.6
2,181,695 + -0.3
2,181,726 + -1.8
2,181,731 - -0.1
2,181,749 - -0.3
2,181,801 - -0.5
2,181,806 - -0.0
2,181,875 + thiD b2103 0.17 -0.2
2,181,883 - thiD b2103 0.18 +2.0
2,181,883 - thiD b2103 0.18 -0.1
2,181,883 - thiD b2103 0.18 -1.4
2,182,009 + thiD b2103 0.34 +1.1
2,182,009 + thiD b2103 0.34 -1.5
2,182,012 + thiD b2103 0.34 -0.7
2,182,020 - thiD b2103 0.35 -0.5
2,182,119 - thiD b2103 0.48 -0.3
2,182,150 - thiD b2103 0.51 -0.3
2,182,161 + thiD b2103 0.53 -0.0
2,182,375 + thiD b2103 0.80 -0.3
2,182,375 + thiD b2103 0.80 -1.8
2,182,382 + thiD b2103 0.80 +0.5
2,182,546 - -0.1
2,182,646 + thiM b2104 0.14 -0.5
2,183,006 - thiM b2104 0.60 -1.5
2,183,006 - thiM b2104 0.60 +0.2
2,183,044 + thiM b2104 0.65 -0.4
2,183,246 + +0.1
2,183,246 + +1.4
2,183,259 - +0.3
2,183,259 - +0.5
2,183,389 - -0.8
2,183,399 - -1.9
2,183,399 - -0.7
2,183,399 - +0.3
2,183,495 - +0.4
2,183,533 - +0.5
2,183,614 + yohL b2105 0.25 -1.8
2,183,614 - yohL b2105 0.25 -0.6
2,183,620 - yohL b2105 0.27 -0.1
2,183,621 + yohL b2105 0.27 +0.5
2,183,766 - yohL b2105 0.81 +0.1
2,183,814 - -1.7
2,183,814 - -1.2
2,183,989 + -0.4
2,184,011 + -0.1
2,184,014 + +0.3
2,184,014 + -1.0
2,184,030 - yohM b2106 0.11 -0.9
2,184,141 + yohM b2106 0.24 +0.4
2,184,141 + yohM b2106 0.24 +0.9
2,184,272 + yohM b2106 0.40 -2.8

Or see this region's nucleotide sequence