Strain Fitness in Escherichia coli BW25113 around b0400

Experiment: Calbicans

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntsbcD and phoB are separated by 189 nucleotidesphoB and phoR are separated by 57 nucleotidesphoR and brnQ are separated by 406 nucleotides b0398: sbcD - exonuclease, dsDNA, ATP-dependent (NCBI), at 414,974 to 416,176 sbcD b0399: phoB - DNA-binding response regulator in two-component regulatory system with PhoR (or CreC) (NCBI), at 416,366 to 417,055 phoB b0400: phoR - sensory histidine kinase in two-component regulatory system with PhoB (NCBI), at 417,113 to 418,408 phoR b0401: brnQ - predicted branched chain amino acid transporter (LIV-II) (NCBI), at 418,815 to 420,134 brnQ Position (kb) 417 418 419Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 416.171 kb on + strandat 416.173 kb on + strandat 416.176 kb on - strandat 416.271 kb on - strandat 416.271 kb on - strandat 416.296 kb on - strandat 416.333 kb on - strandat 416.373 kb on + strandat 416.416 kb on + strandat 416.424 kb on - strandat 416.481 kb on - strand, within phoBat 416.481 kb on - strand, within phoBat 416.536 kb on - strand, within phoBat 416.562 kb on - strand, within phoBat 416.562 kb on - strand, within phoBat 416.654 kb on - strand, within phoBat 416.673 kb on - strand, within phoBat 416.676 kb on - strand, within phoBat 416.753 kb on - strandat 416.881 kb on + strand, within phoBat 416.937 kb on - strand, within phoBat 416.971 kb on + strand, within phoBat 416.990 kb on + strandat 416.993 kb on + strandat 417.003 kb on + strandat 417.008 kb on + strandat 417.015 kb on - strandat 417.015 kb on - strandat 417.015 kb on - strandat 417.016 kb on - strandat 417.022 kb on + strandat 417.076 kb on + strandat 417.076 kb on + strandat 417.091 kb on - strandat 417.144 kb on + strandat 417.174 kb on + strandat 417.270 kb on + strand, within phoRat 417.316 kb on - strand, within phoRat 417.389 kb on - strand, within phoRat 417.446 kb on + strand, within phoRat 417.446 kb on + strand, within phoRat 417.453 kb on - strand, within phoRat 417.538 kb on + strand, within phoRat 417.538 kb on + strand, within phoRat 417.541 kb on + strand, within phoRat 417.604 kb on + strand, within phoRat 417.616 kb on + strand, within phoRat 417.660 kb on - strand, within phoRat 417.660 kb on - strand, within phoRat 417.715 kb on - strand, within phoRat 417.715 kb on - strand, within phoRat 417.754 kb on - strand, within phoRat 417.754 kb on - strand, within phoRat 417.783 kb on + strand, within phoRat 417.889 kb on + strand, within phoRat 418.088 kb on + strand, within phoRat 418.096 kb on - strand, within phoRat 418.096 kb on - strand, within phoRat 418.144 kb on - strand, within phoRat 418.148 kb on + strand, within phoRat 418.175 kb on + strand, within phoRat 418.183 kb on - strand, within phoRat 418.183 kb on - strand, within phoRat 418.191 kb on + strand, within phoRat 418.191 kb on + strand, within phoRat 418.241 kb on + strand, within phoRat 418.283 kb on - strandat 418.327 kb on + strandat 418.333 kb on + strandat 418.333 kb on + strandat 418.336 kb on - strandat 418.346 kb on - strandat 418.423 kb on - strandat 418.611 kb on + strandat 418.658 kb on - strandat 418.794 kb on + strandat 418.856 kb on - strandat 418.899 kb on - strandat 418.899 kb on - strandat 418.915 kb on + strandat 418.942 kb on - strandat 418.949 kb on + strand, within brnQat 418.969 kb on + strand, within brnQat 418.992 kb on + strand, within brnQat 419.112 kb on - strand, within brnQat 419.153 kb on + strand, within brnQat 419.195 kb on + strand, within brnQat 419.243 kb on + strand, within brnQat 419.249 kb on - strand, within brnQat 419.263 kb on + strand, within brnQat 419.263 kb on + strand, within brnQat 419.308 kb on + strand, within brnQat 419.328 kb on + strand, within brnQat 419.404 kb on + strand, within brnQ

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Calbicans
remove
416,171 + -0.7
416,173 + -0.4
416,176 - -0.0
416,271 - -2.8
416,271 - -0.1
416,296 - -0.4
416,333 - -0.6
416,373 + +0.1
416,416 + -1.9
416,424 - +0.2
416,481 - phoB b0399 0.17 -0.5
416,481 - phoB b0399 0.17 -1.1
416,536 - phoB b0399 0.25 -0.6
416,562 - phoB b0399 0.28 -0.1
416,562 - phoB b0399 0.28 -0.5
416,654 - phoB b0399 0.42 -0.8
416,673 - phoB b0399 0.44 -0.6
416,676 - phoB b0399 0.45 -1.3
416,753 - -0.0
416,881 + phoB b0399 0.75 +0.2
416,937 - phoB b0399 0.83 -0.7
416,971 + phoB b0399 0.88 -0.1
416,990 + +0.3
416,993 + -0.3
417,003 + -0.7
417,008 + -1.4
417,015 - +1.0
417,015 - +0.4
417,015 - -0.0
417,016 - -1.6
417,022 + +0.8
417,076 + +0.8
417,076 + -0.2
417,091 - -0.9
417,144 + +0.4
417,174 + -2.6
417,270 + phoR b0400 0.12 -0.8
417,316 - phoR b0400 0.16 -1.4
417,389 - phoR b0400 0.21 -0.5
417,446 + phoR b0400 0.26 +0.6
417,446 + phoR b0400 0.26 +0.6
417,453 - phoR b0400 0.26 -1.6
417,538 + phoR b0400 0.33 -0.3
417,538 + phoR b0400 0.33 +1.6
417,541 + phoR b0400 0.33 -0.4
417,604 + phoR b0400 0.38 +1.2
417,616 + phoR b0400 0.39 -0.3
417,660 - phoR b0400 0.42 -0.4
417,660 - phoR b0400 0.42 -3.3
417,715 - phoR b0400 0.46 -0.0
417,715 - phoR b0400 0.46 -2.4
417,754 - phoR b0400 0.49 -0.9
417,754 - phoR b0400 0.49 -0.6
417,783 + phoR b0400 0.52 -2.9
417,889 + phoR b0400 0.60 -0.7
418,088 + phoR b0400 0.75 -3.5
418,096 - phoR b0400 0.76 -1.3
418,096 - phoR b0400 0.76 -1.5
418,144 - phoR b0400 0.80 -1.9
418,148 + phoR b0400 0.80 -2.2
418,175 + phoR b0400 0.82 -0.7
418,183 - phoR b0400 0.83 -2.4
418,183 - phoR b0400 0.83 -0.1
418,191 + phoR b0400 0.83 -2.4
418,191 + phoR b0400 0.83 +0.2
418,241 + phoR b0400 0.87 -2.4
418,283 - -0.5
418,327 + -3.4
418,333 + -3.9
418,333 + -0.5
418,336 - -0.7
418,346 - -0.9
418,423 - +0.1
418,611 + +0.4
418,658 - +0.2
418,794 + -0.6
418,856 - +0.1
418,899 - -0.6
418,899 - -1.8
418,915 + -0.3
418,942 - +0.5
418,949 + brnQ b0401 0.10 -0.4
418,969 + brnQ b0401 0.12 +0.5
418,992 + brnQ b0401 0.13 -0.1
419,112 - brnQ b0401 0.23 -0.9
419,153 + brnQ b0401 0.26 -0.8
419,195 + brnQ b0401 0.29 +1.1
419,243 + brnQ b0401 0.32 -0.1
419,249 - brnQ b0401 0.33 +1.1
419,263 + brnQ b0401 0.34 -1.6
419,263 + brnQ b0401 0.34 -0.7
419,308 + brnQ b0401 0.37 +2.4
419,328 + brnQ b0401 0.39 -0.4
419,404 + brnQ b0401 0.45 +0.3

Or see this region's nucleotide sequence