Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt htrC and sroH are separated by 1 nucleotides sroH and thiH are separated by 247 nucleotides thiH and thiG overlap by 4 nucleotides thiG and thiS are separated by 1 nucleotides thiS and thiF overlap by 17 nucleotides
b3989: htrC - heat shock protein (NCBI), at 4,187,809 to 4,188,348
htrC
b4691: sroH - Novel sRNA, function unknown (RefSeq), at 4,188,350 to 4,188,510
sroH
b3990: thiH - thiamine biosynthesis protein ThiH (NCBI), at 4,188,758 to 4,189,891
thiH
b3991: thiG - thiamin biosynthesis, thiazole moiety (VIMSS), at 4,189,888 to 4,190,658
thiG
b4407: thiS - sulfur carrier protein ThiS (NCBI), at 4,190,660 to 4,190,860
thiS
b3992: thiF - thiamin biosynthesis, thiazole moiety (VIMSS), at 4,190,844 to 4,191,599
thiF
Position (kb)
4188
4189
4190 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4187.774 kb on + strand at 4187.774 kb on + strand at 4187.793 kb on + strand at 4187.896 kb on + strand, within htrC at 4187.904 kb on - strand, within htrC at 4187.904 kb on - strand, within htrC at 4187.916 kb on - strand, within htrC at 4187.949 kb on + strand, within htrC at 4187.957 kb on - strand, within htrC at 4187.957 kb on - strand, within htrC at 4187.994 kb on - strand, within htrC at 4187.994 kb on - strand, within htrC at 4188.013 kb on + strand, within htrC at 4188.013 kb on + strand, within htrC at 4188.034 kb on - strand, within htrC at 4188.034 kb on - strand, within htrC at 4188.049 kb on + strand, within htrC at 4188.066 kb on - strand, within htrC at 4188.087 kb on - strand, within htrC at 4188.087 kb on - strand, within htrC at 4188.087 kb on - strand, within htrC at 4188.130 kb on + strand, within htrC at 4188.150 kb on + strand, within htrC at 4188.160 kb on + strand, within htrC at 4188.168 kb on - strand, within htrC at 4188.168 kb on - strand, within htrC at 4188.196 kb on - strand, within htrC at 4188.239 kb on + strand, within htrC at 4188.309 kb on - strand at 4188.309 kb on - strand at 4188.365 kb on - strand at 4188.365 kb on - strand at 4188.565 kb on + strand at 4188.579 kb on - strand at 4188.751 kb on - strand at 4188.765 kb on - strand at 4188.812 kb on + strand at 4188.870 kb on + strand at 4188.899 kb on - strand, within thiH at 4189.035 kb on - strand, within thiH at 4189.048 kb on + strand, within thiH at 4189.056 kb on - strand, within thiH at 4189.073 kb on - strand, within thiH at 4189.133 kb on - strand, within thiH at 4189.334 kb on - strand, within thiH at 4189.351 kb on - strand, within thiH at 4189.351 kb on - strand, within thiH at 4189.362 kb on - strand, within thiH at 4189.372 kb on - strand, within thiH at 4189.372 kb on - strand, within thiH at 4189.429 kb on + strand, within thiH at 4189.429 kb on - strand, within thiH at 4189.429 kb on - strand, within thiH at 4189.495 kb on + strand, within thiH at 4189.506 kb on + strand, within thiH at 4189.508 kb on + strand, within thiH at 4189.543 kb on - strand, within thiH at 4189.543 kb on - strand, within thiH at 4189.724 kb on + strand, within thiH at 4189.737 kb on - strand, within thiH at 4189.737 kb on - strand, within thiH at 4189.759 kb on - strand, within thiH at 4189.759 kb on - strand, within thiH at 4190.005 kb on - strand, within thiG at 4190.005 kb on - strand, within thiG at 4190.187 kb on - strand, within thiG at 4190.187 kb on - strand, within thiG at 4190.253 kb on + strand, within thiG at 4190.267 kb on - strand, within thiG at 4190.267 kb on - strand, within thiG at 4190.270 kb on + strand, within thiG at 4190.270 kb on + strand, within thiG at 4190.278 kb on - strand, within thiG at 4190.278 kb on - strand, within thiG at 4190.287 kb on - strand, within thiG at 4190.306 kb on - strand, within thiG at 4190.306 kb on - strand, within thiG at 4190.404 kb on + strand, within thiG at 4190.412 kb on - strand, within thiG at 4190.528 kb on - strand, within thiG at 4190.579 kb on + strand, within thiG at 4190.581 kb on + strand, within thiG at 4190.581 kb on + strand, within thiG at 4190.584 kb on - strand at 4190.586 kb on - strand at 4190.791 kb on + strand, within thiS at 4190.820 kb on + strand, within thiS at 4190.830 kb on + strand, within thiS at 4190.860 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,187,774 + +1.0 4,187,774 + +0.3 4,187,793 + -1.1 4,187,896 + htrC b3989 0.16 -0.4 4,187,904 - htrC b3989 0.18 +1.2 4,187,904 - htrC b3989 0.18 +0.8 4,187,916 - htrC b3989 0.20 +0.3 4,187,949 + htrC b3989 0.26 +0.0 4,187,957 - htrC b3989 0.27 -0.3 4,187,957 - htrC b3989 0.27 -0.2 4,187,994 - htrC b3989 0.34 +0.2 4,187,994 - htrC b3989 0.34 +0.6 4,188,013 + htrC b3989 0.38 -0.9 4,188,013 + htrC b3989 0.38 +0.7 4,188,034 - htrC b3989 0.42 -0.0 4,188,034 - htrC b3989 0.42 +0.9 4,188,049 + htrC b3989 0.44 -0.1 4,188,066 - htrC b3989 0.48 -1.2 4,188,087 - htrC b3989 0.51 +0.1 4,188,087 - htrC b3989 0.51 +0.1 4,188,087 - htrC b3989 0.51 +0.8 4,188,130 + htrC b3989 0.59 -0.2 4,188,150 + htrC b3989 0.63 +0.4 4,188,160 + htrC b3989 0.65 -3.1 4,188,168 - htrC b3989 0.66 -0.4 4,188,168 - htrC b3989 0.66 -0.3 4,188,196 - htrC b3989 0.72 -1.9 4,188,239 + htrC b3989 0.80 -0.2 4,188,309 - -0.8 4,188,309 - +1.6 4,188,365 - -2.1 4,188,365 - -0.9 4,188,565 + -0.0 4,188,579 - -0.1 4,188,751 - -0.6 4,188,765 - -0.2 4,188,812 + -0.5 4,188,870 + -1.6 4,188,899 - thiH b3990 0.12 -0.5 4,189,035 - thiH b3990 0.24 -0.4 4,189,048 + thiH b3990 0.26 -1.2 4,189,056 - thiH b3990 0.26 -0.7 4,189,073 - thiH b3990 0.28 +0.4 4,189,133 - thiH b3990 0.33 +0.3 4,189,334 - thiH b3990 0.51 -2.6 4,189,351 - thiH b3990 0.52 +0.3 4,189,351 - thiH b3990 0.52 -3.3 4,189,362 - thiH b3990 0.53 -0.9 4,189,372 - thiH b3990 0.54 -0.3 4,189,372 - thiH b3990 0.54 -2.0 4,189,429 + thiH b3990 0.59 -0.7 4,189,429 - thiH b3990 0.59 -0.9 4,189,429 - thiH b3990 0.59 -2.6 4,189,495 + thiH b3990 0.65 -2.3 4,189,506 + thiH b3990 0.66 -3.2 4,189,508 + thiH b3990 0.66 -0.2 4,189,543 - thiH b3990 0.69 -1.8 4,189,543 - thiH b3990 0.69 -1.4 4,189,724 + thiH b3990 0.85 -3.3 4,189,737 - thiH b3990 0.86 -2.4 4,189,737 - thiH b3990 0.86 -0.1 4,189,759 - thiH b3990 0.88 -1.7 4,189,759 - thiH b3990 0.88 +0.2 4,190,005 - thiG b3991 0.15 -1.5 4,190,005 - thiG b3991 0.15 -1.2 4,190,187 - thiG b3991 0.39 -1.8 4,190,187 - thiG b3991 0.39 +0.6 4,190,253 + thiG b3991 0.47 -1.3 4,190,267 - thiG b3991 0.49 +0.6 4,190,267 - thiG b3991 0.49 -1.4 4,190,270 + thiG b3991 0.50 -0.7 4,190,270 + thiG b3991 0.50 -1.6 4,190,278 - thiG b3991 0.51 -1.5 4,190,278 - thiG b3991 0.51 -1.5 4,190,287 - thiG b3991 0.52 -2.3 4,190,306 - thiG b3991 0.54 -1.5 4,190,306 - thiG b3991 0.54 -2.8 4,190,404 + thiG b3991 0.67 -3.9 4,190,412 - thiG b3991 0.68 -2.1 4,190,528 - thiG b3991 0.83 -0.0 4,190,579 + thiG b3991 0.90 -3.0 4,190,581 + thiG b3991 0.90 -4.6 4,190,581 + thiG b3991 0.90 -5.2 4,190,584 - -0.8 4,190,586 - -3.0 4,190,791 + thiS b4407 0.65 -1.3 4,190,820 + thiS b4407 0.80 -1.3 4,190,830 + thiS b4407 0.85 -1.5 4,190,860 - -0.5
Or see this region's nucleotide sequence