Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rttR and tpr overlap by 150 nucleotides tpr and tyrV are separated by 67 nucleotides tyrV and tyrT are separated by 209 nucleotides tyrT and purU are separated by 159 nucleotides
b4425: rttR - regulatory sRNA (NCBI), at 1,286,289 to 1,286,459
rttR
b1229: tpr - predicted protamine-like protein (RefSeq), at 1,286,310 to 1,286,399
tpr
b1230: tyrV - tRNA-Tyr (NCBI), at 1,286,467 to 1,286,551
tyrV
b1231: tyrT - tRNA-Tyr (NCBI), at 1,286,761 to 1,286,845
tyrT
b1232: purU - formyltetrahydrofolate deformylase (NCBI), at 1,287,005 to 1,287,847
purU
Position (kb)
1286
1287 Strain fitness (log2 ratio)
-2
-1
0
1 at 1286.125 kb on + strand at 1286.316 kb on - strand at 1286.316 kb on - strand at 1286.690 kb on - strand at 1286.690 kb on - strand at 1286.805 kb on + strand, within tyrT at 1286.916 kb on + strand at 1287.056 kb on + strand at 1287.064 kb on + strand at 1287.233 kb on + strand, within purU at 1287.255 kb on - strand, within purU at 1287.255 kb on - strand, within purU at 1287.342 kb on + strand, within purU at 1287.375 kb on + strand, within purU at 1287.423 kb on - strand, within purU at 1287.425 kb on + strand, within purU at 1287.439 kb on + strand, within purU at 1287.448 kb on + strand, within purU at 1287.473 kb on - strand, within purU at 1287.491 kb on + strand, within purU at 1287.647 kb on - strand, within purU at 1287.691 kb on - strand, within purU at 1287.714 kb on + strand, within purU at 1287.801 kb on - strand at 1287.801 kb on - strand at 1287.837 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,286,125 + +0.7 1,286,316 - +1.2 1,286,316 - -2.0 1,286,690 - -0.0 1,286,690 - +0.9 1,286,805 + tyrT b1231 0.52 +0.7 1,286,916 + -0.9 1,287,056 + -1.2 1,287,064 + -2.5 1,287,233 + purU b1232 0.27 -0.2 1,287,255 - purU b1232 0.30 -1.0 1,287,255 - purU b1232 0.30 -0.6 1,287,342 + purU b1232 0.40 -0.9 1,287,375 + purU b1232 0.44 -1.8 1,287,423 - purU b1232 0.50 +0.4 1,287,425 + purU b1232 0.50 +0.0 1,287,439 + purU b1232 0.51 -0.2 1,287,448 + purU b1232 0.53 -0.6 1,287,473 - purU b1232 0.56 -0.1 1,287,491 + purU b1232 0.58 -1.2 1,287,647 - purU b1232 0.76 +0.1 1,287,691 - purU b1232 0.81 -0.5 1,287,714 + purU b1232 0.84 -0.2 1,287,801 - -0.0 1,287,801 - -0.3 1,287,837 + +0.2
Or see this region's nucleotide sequence