Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt iadA and yjiG are separated by 12 nucleotides yjiG and yjiH overlap by 4 nucleotides yjiH and yjiI are separated by 249 nucleotides yjiI and yjiJ are separated by 12 nucleotides
b4328: iadA - isoaspartyl dipeptidase (NCBI), at 4,556,377 to 4,557,549
iadA
b4329: yjiG - conserved inner membrane protein (NCBI), at 4,557,562 to 4,558,023
yjiG
b4330: yjiH - orf, hypothetical protein (VIMSS), at 4,558,020 to 4,558,703
yjiH
b4331: yjiI - orf, hypothetical protein (VIMSS), at 4,558,953 to 4,559,507
yjiI
b4332: yjiJ - predicted inner membrane protein (NCBI), at 4,559,520 to 4,560,698
yjiJ
Position (kb)
4558
4559 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4557.054 kb on - strand, within iadA at 4557.054 kb on - strand, within iadA at 4557.073 kb on + strand, within iadA at 4557.120 kb on - strand, within iadA at 4557.138 kb on + strand, within iadA at 4557.246 kb on - strand, within iadA at 4557.249 kb on + strand, within iadA at 4557.257 kb on - strand, within iadA at 4557.509 kb on + strand at 4557.623 kb on - strand, within yjiG at 4557.665 kb on - strand, within yjiG at 4557.665 kb on - strand, within yjiG at 4557.667 kb on - strand, within yjiG at 4557.793 kb on + strand, within yjiG at 4557.793 kb on + strand, within yjiG at 4557.801 kb on - strand, within yjiG at 4557.801 kb on - strand, within yjiG at 4557.837 kb on - strand, within yjiG at 4557.837 kb on - strand, within yjiG at 4558.050 kb on - strand at 4558.104 kb on - strand, within yjiH at 4558.116 kb on + strand, within yjiH at 4558.181 kb on - strand, within yjiH at 4558.185 kb on + strand, within yjiH at 4558.211 kb on - strand, within yjiH at 4558.301 kb on + strand, within yjiH at 4558.328 kb on + strand, within yjiH at 4558.392 kb on + strand, within yjiH at 4558.392 kb on + strand, within yjiH at 4558.428 kb on + strand, within yjiH at 4558.741 kb on - strand at 4558.880 kb on - strand at 4559.040 kb on + strand, within yjiI at 4559.040 kb on + strand, within yjiI at 4559.045 kb on - strand, within yjiI at 4559.050 kb on + strand, within yjiI at 4559.050 kb on + strand, within yjiI at 4559.082 kb on + strand, within yjiI at 4559.082 kb on + strand, within yjiI at 4559.113 kb on + strand, within yjiI at 4559.141 kb on - strand, within yjiI at 4559.183 kb on - strand, within yjiI at 4559.196 kb on + strand, within yjiI at 4559.204 kb on - strand, within yjiI at 4559.204 kb on - strand, within yjiI at 4559.204 kb on - strand, within yjiI at 4559.286 kb on - strand, within yjiI at 4559.286 kb on - strand, within yjiI at 4559.338 kb on - strand, within yjiI at 4559.340 kb on - strand, within yjiI at 4559.361 kb on - strand, within yjiI at 4559.482 kb on - strand at 4559.542 kb on - strand at 4559.557 kb on + strand at 4559.575 kb on + strand at 4559.575 kb on + strand at 4559.644 kb on - strand, within yjiJ at 4559.649 kb on - strand, within yjiJ at 4559.651 kb on - strand, within yjiJ at 4559.690 kb on - strand, within yjiJ
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,557,054 - iadA b4328 0.58 -4.1 4,557,054 - iadA b4328 0.58 -5.0 4,557,073 + iadA b4328 0.59 +0.3 4,557,120 - iadA b4328 0.63 +0.4 4,557,138 + iadA b4328 0.65 -0.5 4,557,246 - iadA b4328 0.74 -1.4 4,557,249 + iadA b4328 0.74 +0.3 4,557,257 - iadA b4328 0.75 -0.3 4,557,509 + +0.0 4,557,623 - yjiG b4329 0.13 -0.2 4,557,665 - yjiG b4329 0.22 +0.7 4,557,665 - yjiG b4329 0.22 +0.3 4,557,667 - yjiG b4329 0.23 -1.0 4,557,793 + yjiG b4329 0.50 -0.4 4,557,793 + yjiG b4329 0.50 +0.8 4,557,801 - yjiG b4329 0.52 -0.2 4,557,801 - yjiG b4329 0.52 +0.5 4,557,837 - yjiG b4329 0.60 -0.1 4,557,837 - yjiG b4329 0.60 +0.2 4,558,050 - -1.6 4,558,104 - yjiH b4330 0.12 -1.3 4,558,116 + yjiH b4330 0.14 +1.3 4,558,181 - yjiH b4330 0.24 +0.7 4,558,185 + yjiH b4330 0.24 -0.6 4,558,211 - yjiH b4330 0.28 +0.4 4,558,301 + yjiH b4330 0.41 +0.1 4,558,328 + yjiH b4330 0.45 +1.2 4,558,392 + yjiH b4330 0.54 +0.5 4,558,392 + yjiH b4330 0.54 +1.0 4,558,428 + yjiH b4330 0.60 +0.5 4,558,741 - +0.4 4,558,880 - +0.6 4,559,040 + yjiI b4331 0.16 -1.9 4,559,040 + yjiI b4331 0.16 +0.5 4,559,045 - yjiI b4331 0.17 +0.0 4,559,050 + yjiI b4331 0.17 +0.6 4,559,050 + yjiI b4331 0.17 -0.2 4,559,082 + yjiI b4331 0.23 +0.1 4,559,082 + yjiI b4331 0.23 -0.3 4,559,113 + yjiI b4331 0.29 -0.0 4,559,141 - yjiI b4331 0.34 -0.9 4,559,183 - yjiI b4331 0.41 +0.1 4,559,196 + yjiI b4331 0.44 -0.3 4,559,204 - yjiI b4331 0.45 -0.2 4,559,204 - yjiI b4331 0.45 -3.2 4,559,204 - yjiI b4331 0.45 -0.6 4,559,286 - yjiI b4331 0.60 -0.4 4,559,286 - yjiI b4331 0.60 +0.0 4,559,338 - yjiI b4331 0.69 -0.1 4,559,340 - yjiI b4331 0.70 +0.0 4,559,361 - yjiI b4331 0.74 -0.1 4,559,482 - -0.4 4,559,542 - -0.7 4,559,557 + -0.6 4,559,575 + -0.2 4,559,575 + +0.4 4,559,644 - yjiJ b4332 0.11 +0.7 4,559,649 - yjiJ b4332 0.11 +0.2 4,559,651 - yjiJ b4332 0.11 +0.7 4,559,690 - yjiJ b4332 0.14 +0.2
Or see this region's nucleotide sequence