Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemE and nfi are separated by 9 nucleotides nfi and yjaG are separated by 42 nucleotides yjaG and hupA are separated by 186 nucleotides hupA and yjaH are separated by 12 nucleotides
b3997: hemE - uroporphyrinogen decarboxylase (NCBI), at 4,195,739 to 4,196,803
hemE
b3998: nfi - endonuclease V (deoxyinosine 3'endoduclease) (VIMSS), at 4,196,813 to 4,197,484
nfi
b3999: yjaG - hypothetical protein (NCBI), at 4,197,527 to 4,198,117
yjaG
b4000: hupA - HU, DNA-binding transcriptional regulator, alpha subunit (NCBI), at 4,198,304 to 4,198,576
hupA
b4001: yjaH - hypothetical protein (NCBI), at 4,198,589 to 4,199,284
yjaH
Position (kb)
4197
4198
4199 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4196.940 kb on - strand, within nfi at 4196.973 kb on - strand, within nfi at 4197.077 kb on + strand, within nfi at 4197.155 kb on - strand, within nfi at 4197.274 kb on - strand, within nfi at 4197.274 kb on - strand, within nfi at 4197.291 kb on + strand, within nfi at 4197.291 kb on + strand, within nfi at 4197.316 kb on + strand, within nfi at 4197.331 kb on + strand, within nfi at 4197.353 kb on + strand, within nfi at 4197.353 kb on + strand, within nfi at 4197.353 kb on + strand, within nfi at 4197.361 kb on - strand, within nfi at 4197.361 kb on - strand, within nfi at 4197.383 kb on - strand, within nfi at 4197.383 kb on - strand, within nfi at 4197.436 kb on - strand at 4197.436 kb on - strand at 4197.471 kb on - strand at 4197.593 kb on - strand, within yjaG at 4197.608 kb on + strand, within yjaG at 4197.618 kb on - strand, within yjaG at 4197.618 kb on - strand, within yjaG at 4197.733 kb on - strand, within yjaG at 4197.855 kb on - strand, within yjaG at 4197.855 kb on - strand, within yjaG at 4197.871 kb on + strand, within yjaG at 4197.879 kb on - strand, within yjaG at 4197.911 kb on + strand, within yjaG at 4197.911 kb on + strand, within yjaG at 4197.983 kb on - strand, within yjaG at 4198.028 kb on + strand, within yjaG at 4198.028 kb on + strand, within yjaG at 4198.070 kb on - strand at 4198.162 kb on + strand at 4198.235 kb on - strand at 4198.245 kb on - strand at 4198.250 kb on - strand at 4198.266 kb on + strand at 4198.287 kb on - strand at 4198.287 kb on - strand at 4198.359 kb on - strand, within hupA at 4198.359 kb on - strand, within hupA at 4198.380 kb on + strand, within hupA at 4198.380 kb on + strand, within hupA at 4198.388 kb on - strand, within hupA at 4198.409 kb on + strand, within hupA at 4198.409 kb on + strand, within hupA at 4198.425 kb on + strand, within hupA at 4198.443 kb on - strand, within hupA at 4198.457 kb on - strand, within hupA at 4198.577 kb on - strand at 4198.579 kb on - strand at 4198.579 kb on - strand at 4198.581 kb on + strand at 4198.581 kb on + strand at 4198.824 kb on + strand, within yjaH at 4198.824 kb on + strand, within yjaH at 4198.833 kb on + strand, within yjaH at 4198.833 kb on + strand, within yjaH at 4198.925 kb on + strand, within yjaH at 4198.932 kb on + strand, within yjaH at 4198.996 kb on + strand, within yjaH at 4199.081 kb on - strand, within yjaH
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,196,940 - nfi b3998 0.19 -0.5 4,196,973 - nfi b3998 0.24 -0.3 4,197,077 + nfi b3998 0.39 -0.2 4,197,155 - nfi b3998 0.51 +0.8 4,197,274 - nfi b3998 0.69 +0.4 4,197,274 - nfi b3998 0.69 -1.8 4,197,291 + nfi b3998 0.71 -1.2 4,197,291 + nfi b3998 0.71 +0.4 4,197,316 + nfi b3998 0.75 +0.0 4,197,331 + nfi b3998 0.77 +0.4 4,197,353 + nfi b3998 0.80 +0.2 4,197,353 + nfi b3998 0.80 +0.4 4,197,353 + nfi b3998 0.80 +0.8 4,197,361 - nfi b3998 0.82 +0.2 4,197,361 - nfi b3998 0.82 -0.8 4,197,383 - nfi b3998 0.85 -0.1 4,197,383 - nfi b3998 0.85 +0.9 4,197,436 - -0.8 4,197,436 - -0.8 4,197,471 - +0.5 4,197,593 - yjaG b3999 0.11 +0.9 4,197,608 + yjaG b3999 0.14 +0.4 4,197,618 - yjaG b3999 0.15 +0.6 4,197,618 - yjaG b3999 0.15 +0.6 4,197,733 - yjaG b3999 0.35 -0.1 4,197,855 - yjaG b3999 0.55 +1.2 4,197,855 - yjaG b3999 0.55 +0.7 4,197,871 + yjaG b3999 0.58 +1.2 4,197,879 - yjaG b3999 0.60 -0.9 4,197,911 + yjaG b3999 0.65 +1.2 4,197,911 + yjaG b3999 0.65 -0.5 4,197,983 - yjaG b3999 0.77 +0.1 4,198,028 + yjaG b3999 0.85 +1.5 4,198,028 + yjaG b3999 0.85 -0.2 4,198,070 - +0.2 4,198,162 + -0.0 4,198,235 - -2.9 4,198,245 - -0.3 4,198,250 - -0.6 4,198,266 + -0.5 4,198,287 - +0.1 4,198,287 - -0.2 4,198,359 - hupA b4000 0.20 -0.7 4,198,359 - hupA b4000 0.20 +0.1 4,198,380 + hupA b4000 0.28 +0.1 4,198,380 + hupA b4000 0.28 +0.5 4,198,388 - hupA b4000 0.31 +1.3 4,198,409 + hupA b4000 0.38 +0.5 4,198,409 + hupA b4000 0.38 -0.6 4,198,425 + hupA b4000 0.44 -0.2 4,198,443 - hupA b4000 0.51 -2.7 4,198,457 - hupA b4000 0.56 +0.3 4,198,577 - -2.0 4,198,579 - +0.1 4,198,579 - +1.0 4,198,581 + -0.2 4,198,581 + -0.7 4,198,824 + yjaH b4001 0.34 +0.5 4,198,824 + yjaH b4001 0.34 -0.8 4,198,833 + yjaH b4001 0.35 +0.3 4,198,833 + yjaH b4001 0.35 +0.7 4,198,925 + yjaH b4001 0.48 +0.7 4,198,932 + yjaH b4001 0.49 +0.7 4,198,996 + yjaH b4001 0.58 -1.6 4,199,081 - yjaH b4001 0.71 +0.3
Or see this region's nucleotide sequence