Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cpdA and yqiB are separated by 24 nucleotides yqiB and nudF are separated by 0 nucleotides nudF and tolC are separated by 204 nucleotides
b3032: cpdA - cyclic 3',5'-adenosine monophosphate phosphodiesterase (NCBI), at 3,174,028 to 3,174,855
cpdA
b3033: yqiB - predicted dehydrogenase (NCBI), at 3,174,880 to 3,175,302
yqiB
b3034: nudF - ADP-ribose pyrophosphatase (NCBI), at 3,175,303 to 3,175,932
nudF
b3035: tolC - outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS), at 3,176,137 to 3,177,618
tolC
Position (kb)
3175
3176 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3174.303 kb on - strand, within cpdA at 3174.332 kb on + strand, within cpdA at 3174.332 kb on + strand, within cpdA at 3174.373 kb on - strand, within cpdA at 3174.373 kb on - strand, within cpdA at 3174.405 kb on + strand, within cpdA at 3174.433 kb on - strand, within cpdA at 3174.457 kb on + strand, within cpdA at 3174.503 kb on + strand, within cpdA at 3174.506 kb on + strand, within cpdA at 3174.647 kb on + strand, within cpdA at 3174.694 kb on + strand, within cpdA at 3174.749 kb on + strand, within cpdA at 3174.749 kb on + strand, within cpdA at 3174.749 kb on + strand, within cpdA at 3174.791 kb on - strand at 3174.825 kb on - strand at 3174.825 kb on - strand at 3174.905 kb on - strand at 3174.915 kb on - strand at 3174.927 kb on - strand, within yqiB at 3175.065 kb on + strand, within yqiB at 3175.116 kb on + strand, within yqiB at 3175.126 kb on + strand, within yqiB at 3175.171 kb on - strand, within yqiB at 3175.226 kb on + strand, within yqiB at 3175.399 kb on - strand, within nudF at 3175.469 kb on - strand, within nudF at 3175.494 kb on - strand, within nudF at 3175.494 kb on - strand, within nudF at 3175.528 kb on - strand, within nudF at 3175.577 kb on + strand, within nudF at 3175.585 kb on + strand, within nudF at 3175.590 kb on - strand, within nudF at 3175.662 kb on + strand, within nudF at 3175.679 kb on + strand, within nudF at 3175.679 kb on + strand, within nudF at 3175.752 kb on - strand, within nudF at 3175.798 kb on + strand, within nudF at 3175.818 kb on - strand, within nudF at 3175.823 kb on - strand, within nudF at 3175.871 kb on - strand at 3176.030 kb on - strand at 3176.031 kb on + strand at 3176.119 kb on - strand at 3176.119 kb on - strand at 3176.173 kb on - strand at 3176.173 kb on - strand at 3176.174 kb on + strand at 3176.182 kb on - strand at 3176.208 kb on - strand at 3176.271 kb on + strand at 3176.271 kb on + strand at 3176.304 kb on + strand, within tolC at 3176.309 kb on + strand, within tolC at 3176.309 kb on + strand, within tolC at 3176.349 kb on - strand, within tolC at 3176.378 kb on + strand, within tolC at 3176.493 kb on + strand, within tolC at 3176.526 kb on - strand, within tolC at 3176.571 kb on + strand, within tolC at 3176.573 kb on + strand, within tolC at 3176.581 kb on - strand, within tolC at 3176.635 kb on + strand, within tolC at 3176.638 kb on - strand, within tolC at 3176.654 kb on - strand, within tolC at 3176.680 kb on - strand, within tolC at 3176.680 kb on - strand, within tolC at 3176.682 kb on + strand, within tolC at 3176.692 kb on - strand, within tolC at 3176.716 kb on + strand, within tolC at 3176.716 kb on + strand, within tolC at 3176.844 kb on - strand, within tolC at 3176.844 kb on - strand, within tolC at 3176.869 kb on - strand, within tolC at 3176.869 kb on - strand, within tolC at 3176.887 kb on + strand, within tolC at 3176.887 kb on + strand, within tolC at 3176.887 kb on + strand, within tolC at 3176.932 kb on + strand, within tolC
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 3,174,303 - cpdA b3032 0.33 -2.0 3,174,332 + cpdA b3032 0.37 -0.2 3,174,332 + cpdA b3032 0.37 -1.1 3,174,373 - cpdA b3032 0.42 -3.7 3,174,373 - cpdA b3032 0.42 -0.7 3,174,405 + cpdA b3032 0.46 +0.3 3,174,433 - cpdA b3032 0.49 -1.8 3,174,457 + cpdA b3032 0.52 +0.3 3,174,503 + cpdA b3032 0.57 -0.1 3,174,506 + cpdA b3032 0.58 -0.6 3,174,647 + cpdA b3032 0.75 +0.1 3,174,694 + cpdA b3032 0.80 -1.1 3,174,749 + cpdA b3032 0.87 -0.4 3,174,749 + cpdA b3032 0.87 -0.4 3,174,749 + cpdA b3032 0.87 -1.6 3,174,791 - +0.4 3,174,825 - -0.6 3,174,825 - -0.9 3,174,905 - -0.4 3,174,915 - -0.7 3,174,927 - yqiB b3033 0.11 +0.9 3,175,065 + yqiB b3033 0.44 +0.6 3,175,116 + yqiB b3033 0.56 +0.0 3,175,126 + yqiB b3033 0.58 +0.3 3,175,171 - yqiB b3033 0.69 +0.9 3,175,226 + yqiB b3033 0.82 -0.6 3,175,399 - nudF b3034 0.15 +0.1 3,175,469 - nudF b3034 0.26 +1.5 3,175,494 - nudF b3034 0.30 +2.5 3,175,494 - nudF b3034 0.30 +1.4 3,175,528 - nudF b3034 0.36 +1.3 3,175,577 + nudF b3034 0.43 +0.3 3,175,585 + nudF b3034 0.45 -1.0 3,175,590 - nudF b3034 0.46 +1.2 3,175,662 + nudF b3034 0.57 -0.6 3,175,679 + nudF b3034 0.60 -1.0 3,175,679 + nudF b3034 0.60 -0.8 3,175,752 - nudF b3034 0.71 -0.3 3,175,798 + nudF b3034 0.79 +0.3 3,175,818 - nudF b3034 0.82 -0.2 3,175,823 - nudF b3034 0.83 +0.5 3,175,871 - +0.6 3,176,030 - +0.6 3,176,031 + -1.8 3,176,119 - +1.0 3,176,119 - -0.2 3,176,173 - +1.4 3,176,173 - +1.6 3,176,174 + +0.2 3,176,182 - -2.0 3,176,208 - -0.4 3,176,271 + -2.0 3,176,271 + -1.6 3,176,304 + tolC b3035 0.11 +0.3 3,176,309 + tolC b3035 0.12 -2.6 3,176,309 + tolC b3035 0.12 +0.5 3,176,349 - tolC b3035 0.14 +0.6 3,176,378 + tolC b3035 0.16 +1.0 3,176,493 + tolC b3035 0.24 +1.6 3,176,526 - tolC b3035 0.26 +1.0 3,176,571 + tolC b3035 0.29 +1.2 3,176,573 + tolC b3035 0.29 -2.6 3,176,581 - tolC b3035 0.30 +0.2 3,176,635 + tolC b3035 0.34 -0.8 3,176,638 - tolC b3035 0.34 +1.1 3,176,654 - tolC b3035 0.35 +1.0 3,176,680 - tolC b3035 0.37 +0.4 3,176,680 - tolC b3035 0.37 -0.3 3,176,682 + tolC b3035 0.37 -0.5 3,176,692 - tolC b3035 0.37 +1.5 3,176,716 + tolC b3035 0.39 +1.0 3,176,716 + tolC b3035 0.39 +0.2 3,176,844 - tolC b3035 0.48 -1.9 3,176,844 - tolC b3035 0.48 -1.1 3,176,869 - tolC b3035 0.49 +0.1 3,176,869 - tolC b3035 0.49 -1.3 3,176,887 + tolC b3035 0.51 +0.7 3,176,887 + tolC b3035 0.51 -3.1 3,176,887 + tolC b3035 0.51 -1.5 3,176,932 + tolC b3035 0.54 -4.2
Or see this region's nucleotide sequence