Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt menC and menB overlap by 1 nucleotides menB and yfbB are separated by 14 nucleotides yfbB and menD overlap by 4 nucleotides
b2261: menC - O-succinylbenzoate synthase (NCBI), at 2,373,022 to 2,373,984
menC
b2262: menB - naphthoate synthase (NCBI), at 2,373,984 to 2,374,841
menB
b2263: yfbB - predicted peptidase (NCBI), at 2,374,856 to 2,375,614
yfbB
b2264: menD - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI), at 2,375,611 to 2,377,281
menD
Position (kb)
2374
2375
2376 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2373.925 kb on + strand at 2373.930 kb on + strand at 2373.930 kb on + strand at 2374.136 kb on + strand, within menB at 2374.254 kb on - strand, within menB at 2374.373 kb on + strand, within menB at 2374.491 kb on + strand, within menB at 2374.673 kb on - strand, within menB at 2374.831 kb on + strand at 2374.831 kb on + strand at 2374.885 kb on + strand at 2374.893 kb on - strand at 2375.223 kb on - strand, within yfbB at 2375.307 kb on - strand, within yfbB at 2375.363 kb on - strand, within yfbB at 2375.570 kb on + strand at 2375.570 kb on + strand at 2375.616 kb on - strand at 2375.616 kb on - strand at 2375.795 kb on - strand, within menD at 2376.112 kb on - strand, within menD at 2376.143 kb on - strand, within menD at 2376.143 kb on - strand, within menD at 2376.359 kb on + strand, within menD at 2376.359 kb on + strand, within menD at 2376.515 kb on + strand, within menD at 2376.554 kb on - strand, within menD at 2376.585 kb on + strand, within menD at 2376.585 kb on + strand, within menD at 2376.605 kb on + strand, within menD
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,373,925 + -0.1 2,373,930 + +0.2 2,373,930 + -0.5 2,374,136 + menB b2262 0.18 -0.6 2,374,254 - menB b2262 0.31 -0.8 2,374,373 + menB b2262 0.45 +0.1 2,374,491 + menB b2262 0.59 +0.8 2,374,673 - menB b2262 0.80 +1.1 2,374,831 + -0.3 2,374,831 + -1.2 2,374,885 + -0.0 2,374,893 - +0.9 2,375,223 - yfbB b2263 0.48 -0.1 2,375,307 - yfbB b2263 0.59 +0.7 2,375,363 - yfbB b2263 0.67 +0.4 2,375,570 + -0.3 2,375,570 + +0.2 2,375,616 - +0.1 2,375,616 - -0.2 2,375,795 - menD b2264 0.11 +0.1 2,376,112 - menD b2264 0.30 +0.4 2,376,143 - menD b2264 0.32 +0.4 2,376,143 - menD b2264 0.32 +0.2 2,376,359 + menD b2264 0.45 +0.2 2,376,359 + menD b2264 0.45 +0.2 2,376,515 + menD b2264 0.54 -0.2 2,376,554 - menD b2264 0.56 -3.1 2,376,585 + menD b2264 0.58 -0.9 2,376,585 + menD b2264 0.58 +0.1 2,376,605 + menD b2264 0.59 -1.3
Or see this region's nucleotide sequence