Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pabB and yeaB are separated by 3 nucleotides yeaB and sdaA are separated by 183 nucleotides
b1812: pabB - para-aminobenzoate synthase component I (NCBI), at 1,892,829 to 1,894,190
pabB
b1813: yeaB - predicted NUDIX hydrolase (NCBI), at 1,894,194 to 1,894,772
yeaB
b1814: sdaA - L-serine deaminase I (NCBI), at 1,894,956 to 1,896,320
sdaA
Position (kb)
1894
1895 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1893.663 kb on + strand, within pabB at 1893.663 kb on - strand, within pabB at 1893.819 kb on + strand, within pabB at 1893.819 kb on + strand, within pabB at 1893.960 kb on - strand, within pabB at 1893.960 kb on - strand, within pabB at 1894.035 kb on + strand, within pabB at 1894.301 kb on - strand, within yeaB at 1894.400 kb on + strand, within yeaB at 1894.406 kb on - strand, within yeaB at 1894.419 kb on + strand, within yeaB at 1894.446 kb on + strand, within yeaB at 1894.456 kb on + strand, within yeaB at 1894.500 kb on + strand, within yeaB at 1894.500 kb on + strand, within yeaB at 1894.551 kb on - strand, within yeaB at 1894.579 kb on - strand, within yeaB at 1894.629 kb on - strand, within yeaB at 1894.683 kb on + strand, within yeaB at 1894.683 kb on + strand, within yeaB at 1894.686 kb on - strand, within yeaB at 1894.693 kb on + strand, within yeaB at 1894.693 kb on + strand, within yeaB at 1894.735 kb on + strand at 1894.766 kb on + strand at 1894.766 kb on + strand at 1894.804 kb on + strand at 1894.875 kb on - strand at 1894.875 kb on - strand at 1894.890 kb on + strand at 1894.890 kb on + strand at 1894.915 kb on - strand at 1894.961 kb on - strand at 1894.997 kb on + strand at 1895.002 kb on - strand at 1895.028 kb on - strand at 1895.028 kb on - strand at 1895.039 kb on - strand at 1895.039 kb on - strand at 1895.465 kb on + strand, within sdaA at 1895.498 kb on - strand, within sdaA at 1895.540 kb on - strand, within sdaA at 1895.646 kb on - strand, within sdaA at 1895.646 kb on - strand, within sdaA
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,893,663 + pabB b1812 0.61 -0.1 1,893,663 - pabB b1812 0.61 +0.5 1,893,819 + pabB b1812 0.73 +0.1 1,893,819 + pabB b1812 0.73 +0.4 1,893,960 - pabB b1812 0.83 -1.0 1,893,960 - pabB b1812 0.83 +0.3 1,894,035 + pabB b1812 0.89 -0.3 1,894,301 - yeaB b1813 0.18 +0.6 1,894,400 + yeaB b1813 0.36 +0.6 1,894,406 - yeaB b1813 0.37 +0.0 1,894,419 + yeaB b1813 0.39 +0.3 1,894,446 + yeaB b1813 0.44 +1.0 1,894,456 + yeaB b1813 0.45 +0.9 1,894,500 + yeaB b1813 0.53 +1.0 1,894,500 + yeaB b1813 0.53 +0.5 1,894,551 - yeaB b1813 0.62 +0.1 1,894,579 - yeaB b1813 0.66 -1.0 1,894,629 - yeaB b1813 0.75 +0.5 1,894,683 + yeaB b1813 0.84 +0.5 1,894,683 + yeaB b1813 0.84 +0.9 1,894,686 - yeaB b1813 0.85 +0.4 1,894,693 + yeaB b1813 0.86 +0.6 1,894,693 + yeaB b1813 0.86 +1.5 1,894,735 + -0.1 1,894,766 + -0.7 1,894,766 + -0.5 1,894,804 + +0.2 1,894,875 - -0.3 1,894,875 - +2.8 1,894,890 + +0.1 1,894,890 + -1.1 1,894,915 - +1.2 1,894,961 - -0.6 1,894,997 + +0.0 1,895,002 - +0.3 1,895,028 - -0.4 1,895,028 - +1.0 1,895,039 - -0.9 1,895,039 - +0.4 1,895,465 + sdaA b1814 0.37 -0.7 1,895,498 - sdaA b1814 0.40 +0.9 1,895,540 - sdaA b1814 0.43 +0.4 1,895,646 - sdaA b1814 0.51 +1.4 1,895,646 - sdaA b1814 0.51 -0.6
Or see this region's nucleotide sequence