Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fadD and yeaY are separated by 204 nucleotides yeaY and yeaZ are separated by 39 nucleotides yeaZ and yoaA are separated by 57 nucleotides
b1805: fadD - acyl-CoA synthase (NCBI), at 1,886,085 to 1,887,770
fadD
b1806: yeaY - predicted lipoprotein (NCBI), at 1,887,975 to 1,888,556
yeaY
b1807: yeaZ - predicted peptidase (NCBI), at 1,888,596 to 1,889,291
yeaZ
b1808: yoaA - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 1,889,349 to 1,891,259
yoaA
Position (kb)
1887
1888
1889 Strain fitness (log2 ratio)
-2
-1
0
1 at 1886.976 kb on - strand, within fadD at 1886.976 kb on - strand, within fadD at 1887.085 kb on + strand, within fadD at 1887.087 kb on - strand, within fadD at 1887.098 kb on - strand, within fadD at 1887.130 kb on + strand, within fadD at 1887.130 kb on + strand, within fadD at 1887.187 kb on - strand, within fadD at 1887.187 kb on - strand, within fadD at 1887.261 kb on + strand, within fadD at 1887.261 kb on + strand, within fadD at 1887.321 kb on + strand, within fadD at 1887.381 kb on + strand, within fadD at 1887.381 kb on + strand, within fadD at 1887.445 kb on + strand, within fadD at 1887.453 kb on - strand, within fadD at 1887.453 kb on - strand, within fadD at 1887.455 kb on + strand, within fadD at 1887.455 kb on + strand, within fadD at 1887.461 kb on + strand, within fadD at 1887.762 kb on - strand at 1887.981 kb on + strand at 1888.207 kb on + strand, within yeaY at 1888.428 kb on - strand, within yeaY at 1888.428 kb on - strand, within yeaY at 1888.463 kb on + strand, within yeaY at 1888.586 kb on + strand at 1888.586 kb on + strand at 1889.333 kb on - strand at 1889.364 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,886,976 - fadD b1805 0.53 -2.3 1,886,976 - fadD b1805 0.53 -0.1 1,887,085 + fadD b1805 0.59 -0.5 1,887,087 - fadD b1805 0.59 -0.8 1,887,098 - fadD b1805 0.60 -0.5 1,887,130 + fadD b1805 0.62 +0.6 1,887,130 + fadD b1805 0.62 +0.2 1,887,187 - fadD b1805 0.65 -1.4 1,887,187 - fadD b1805 0.65 +0.3 1,887,261 + fadD b1805 0.70 -0.4 1,887,261 + fadD b1805 0.70 +0.3 1,887,321 + fadD b1805 0.73 -0.2 1,887,381 + fadD b1805 0.77 -0.2 1,887,381 + fadD b1805 0.77 -0.7 1,887,445 + fadD b1805 0.81 -0.8 1,887,453 - fadD b1805 0.81 +0.5 1,887,453 - fadD b1805 0.81 +0.6 1,887,455 + fadD b1805 0.81 -0.8 1,887,455 + fadD b1805 0.81 +1.7 1,887,461 + fadD b1805 0.82 -0.6 1,887,762 - -0.7 1,887,981 + +1.5 1,888,207 + yeaY b1806 0.40 +0.5 1,888,428 - yeaY b1806 0.78 +0.8 1,888,428 - yeaY b1806 0.78 +0.4 1,888,463 + yeaY b1806 0.84 +0.7 1,888,586 + +0.1 1,888,586 + +0.3 1,889,333 - +0.0 1,889,364 - -1.6
Or see this region's nucleotide sequence