Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pfkB and ydiZ are separated by 100 nucleotides ydiZ and yniA are separated by 105 nucleotides yniA and yniB are separated by 40 nucleotides yniB and yniC are separated by 146 nucleotides
b1723: pfkB - 6-phosphofructokinase II; suppressor of pfkA (VIMSS), at 1,804,394 to 1,805,323
pfkB
b1724: ydiZ - hypothetical protein (NCBI), at 1,805,424 to 1,805,714
ydiZ
b1725: yniA - predicted phosphotransferase/kinase (NCBI), at 1,805,820 to 1,806,680
yniA
b1726: yniB - predicted inner membrane protein (NCBI), at 1,806,721 to 1,807,257
yniB
b1727: yniC - predicted hydrolase (NCBI), at 1,807,404 to 1,808,072
yniC
Position (kb)
1805
1806
1807 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1804.911 kb on + strand, within pfkB at 1804.911 kb on + strand, within pfkB at 1804.911 kb on + strand, within pfkB at 1805.063 kb on + strand, within pfkB at 1805.063 kb on + strand, within pfkB at 1805.077 kb on - strand, within pfkB at 1805.136 kb on - strand, within pfkB at 1805.145 kb on + strand, within pfkB at 1805.355 kb on + strand at 1805.471 kb on - strand, within ydiZ at 1805.648 kb on - strand, within ydiZ at 1805.853 kb on + strand at 1805.863 kb on - strand at 1805.863 kb on - strand at 1805.980 kb on - strand, within yniA at 1806.165 kb on - strand, within yniA at 1806.217 kb on - strand, within yniA at 1806.333 kb on + strand, within yniA at 1806.412 kb on + strand, within yniA at 1806.412 kb on + strand, within yniA at 1806.430 kb on - strand, within yniA at 1806.458 kb on + strand, within yniA at 1806.458 kb on + strand, within yniA at 1806.464 kb on + strand, within yniA at 1806.563 kb on + strand, within yniA at 1806.563 kb on + strand, within yniA at 1806.573 kb on + strand, within yniA at 1806.593 kb on - strand, within yniA at 1806.593 kb on - strand, within yniA at 1806.598 kb on - strand at 1806.599 kb on - strand at 1806.599 kb on - strand at 1806.665 kb on + strand at 1806.684 kb on + strand at 1806.684 kb on + strand at 1806.686 kb on - strand at 1806.686 kb on - strand at 1806.687 kb on - strand at 1806.697 kb on + strand at 1806.772 kb on - strand at 1806.781 kb on + strand, within yniB at 1807.061 kb on + strand, within yniB at 1807.103 kb on - strand, within yniB at 1807.241 kb on + strand at 1807.253 kb on + strand at 1807.288 kb on + strand at 1807.353 kb on + strand at 1807.363 kb on + strand at 1807.387 kb on - strand at 1807.387 kb on - strand at 1807.470 kb on + strand at 1807.489 kb on + strand, within yniC
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,804,911 + pfkB b1723 0.56 +0.2 1,804,911 + pfkB b1723 0.56 +0.3 1,804,911 + pfkB b1723 0.56 -0.9 1,805,063 + pfkB b1723 0.72 +0.9 1,805,063 + pfkB b1723 0.72 +0.6 1,805,077 - pfkB b1723 0.73 -1.0 1,805,136 - pfkB b1723 0.80 +0.7 1,805,145 + pfkB b1723 0.81 -0.7 1,805,355 + -0.8 1,805,471 - ydiZ b1724 0.16 -1.2 1,805,648 - ydiZ b1724 0.77 +0.4 1,805,853 + +2.5 1,805,863 - -1.9 1,805,863 - -2.3 1,805,980 - yniA b1725 0.19 +1.0 1,806,165 - yniA b1725 0.40 +1.3 1,806,217 - yniA b1725 0.46 +0.0 1,806,333 + yniA b1725 0.60 +0.3 1,806,412 + yniA b1725 0.69 -0.1 1,806,412 + yniA b1725 0.69 +2.1 1,806,430 - yniA b1725 0.71 +0.3 1,806,458 + yniA b1725 0.74 -0.7 1,806,458 + yniA b1725 0.74 +0.3 1,806,464 + yniA b1725 0.75 +0.8 1,806,563 + yniA b1725 0.86 +1.2 1,806,563 + yniA b1725 0.86 -0.2 1,806,573 + yniA b1725 0.87 +0.4 1,806,593 - yniA b1725 0.90 +1.9 1,806,593 - yniA b1725 0.90 +2.0 1,806,598 - +0.5 1,806,599 - +0.0 1,806,599 - -0.0 1,806,665 + -0.5 1,806,684 + -1.6 1,806,684 + -0.7 1,806,686 - -0.5 1,806,686 - -0.2 1,806,687 - -1.2 1,806,697 + +0.3 1,806,772 - -0.1 1,806,781 + yniB b1726 0.11 -0.5 1,807,061 + yniB b1726 0.63 +1.1 1,807,103 - yniB b1726 0.71 +0.7 1,807,241 + +0.3 1,807,253 + -0.5 1,807,288 + +0.1 1,807,353 + -0.0 1,807,363 + -0.6 1,807,387 - +1.0 1,807,387 - +0.2 1,807,470 + -1.0 1,807,489 + yniC b1727 0.13 -0.1
Or see this region's nucleotide sequence