Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fadK and pps are separated by 56 nucleotides pps and ydiA are separated by 332 nucleotides
b1701: fadK - short chain acyl-CoA synthetase, anaerobic (RefSeq), at 1,781,055 to 1,782,701
fadK
b1702: pps - phosphoenolpyruvate synthase (NCBI), at 1,782,758 to 1,785,136
pps
b1703: ydiA - hypothetical protein (NCBI), at 1,785,469 to 1,786,302
ydiA
Position (kb)
1782
1783
1784
1785
1786 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 1781.882 kb on + strand, within fadK at 1781.898 kb on - strand, within fadK at 1782.138 kb on + strand, within fadK at 1782.237 kb on + strand, within fadK at 1782.273 kb on - strand, within fadK at 1782.275 kb on + strand, within fadK at 1782.275 kb on + strand, within fadK at 1782.284 kb on + strand, within fadK at 1782.308 kb on + strand, within fadK at 1782.308 kb on + strand, within fadK at 1782.313 kb on + strand, within fadK at 1782.317 kb on + strand, within fadK at 1782.409 kb on + strand, within fadK at 1782.409 kb on + strand, within fadK at 1782.445 kb on + strand, within fadK at 1782.448 kb on - strand, within fadK at 1782.485 kb on + strand, within fadK at 1782.485 kb on + strand, within fadK at 1782.485 kb on + strand, within fadK at 1782.509 kb on + strand, within fadK at 1782.510 kb on + strand, within fadK at 1782.510 kb on + strand, within fadK at 1782.532 kb on + strand, within fadK at 1782.579 kb on + strand at 1782.660 kb on + strand at 1782.701 kb on + strand at 1782.726 kb on + strand at 1782.728 kb on - strand at 1782.733 kb on - strand at 1782.777 kb on - strand at 1782.777 kb on - strand at 1782.788 kb on + strand at 1782.796 kb on - strand at 1782.809 kb on - strand at 1782.837 kb on + strand at 1782.838 kb on - strand at 1782.839 kb on + strand at 1782.839 kb on + strand at 1783.228 kb on - strand, within pps at 1783.228 kb on - strand, within pps at 1783.228 kb on - strand, within pps at 1783.342 kb on - strand, within pps at 1783.681 kb on - strand, within pps at 1783.681 kb on - strand, within pps at 1783.852 kb on + strand, within pps at 1783.852 kb on + strand, within pps at 1783.992 kb on + strand, within pps at 1784.093 kb on - strand, within pps at 1784.142 kb on + strand, within pps at 1784.212 kb on + strand, within pps at 1784.322 kb on - strand, within pps at 1784.324 kb on - strand, within pps at 1784.418 kb on - strand at 1784.432 kb on - strand, within pps at 1784.446 kb on - strand, within pps at 1784.446 kb on - strand, within pps at 1784.455 kb on - strand, within pps at 1784.472 kb on - strand, within pps at 1784.598 kb on + strand, within pps at 1784.610 kb on + strand, within pps at 1784.832 kb on + strand, within pps at 1784.832 kb on + strand, within pps at 1784.954 kb on + strand at 1785.015 kb on + strand at 1785.056 kb on + strand at 1785.076 kb on + strand at 1785.101 kb on + strand at 1785.101 kb on + strand at 1785.111 kb on + strand at 1785.268 kb on + strand at 1785.268 kb on + strand at 1785.399 kb on - strand at 1785.739 kb on + strand, within ydiA at 1785.765 kb on + strand, within ydiA at 1785.814 kb on - strand, within ydiA at 1785.826 kb on - strand, within ydiA at 1785.827 kb on - strand, within ydiA at 1785.827 kb on - strand, within ydiA at 1785.918 kb on + strand, within ydiA at 1785.918 kb on + strand, within ydiA
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,781,882 + fadK b1701 0.50 +0.5 1,781,898 - fadK b1701 0.51 -1.5 1,782,138 + fadK b1701 0.66 -0.6 1,782,237 + fadK b1701 0.72 +0.1 1,782,273 - fadK b1701 0.74 -0.3 1,782,275 + fadK b1701 0.74 +0.6 1,782,275 + fadK b1701 0.74 +0.7 1,782,284 + fadK b1701 0.75 +1.0 1,782,308 + fadK b1701 0.76 +0.4 1,782,308 + fadK b1701 0.76 -0.1 1,782,313 + fadK b1701 0.76 -2.2 1,782,317 + fadK b1701 0.77 -0.3 1,782,409 + fadK b1701 0.82 -0.9 1,782,409 + fadK b1701 0.82 -0.3 1,782,445 + fadK b1701 0.84 +0.0 1,782,448 - fadK b1701 0.85 +1.7 1,782,485 + fadK b1701 0.87 -1.8 1,782,485 + fadK b1701 0.87 +0.1 1,782,485 + fadK b1701 0.87 +0.3 1,782,509 + fadK b1701 0.88 +0.6 1,782,510 + fadK b1701 0.88 -0.5 1,782,510 + fadK b1701 0.88 +0.4 1,782,532 + fadK b1701 0.90 +0.4 1,782,579 + -1.9 1,782,660 + -0.9 1,782,701 + +0.1 1,782,726 + +0.7 1,782,728 - -1.8 1,782,733 - +2.7 1,782,777 - -0.0 1,782,777 - +0.5 1,782,788 + +2.1 1,782,796 - -2.4 1,782,809 - -3.2 1,782,837 + -3.2 1,782,838 - -4.1 1,782,839 + -3.1 1,782,839 + -4.9 1,783,228 - pps b1702 0.20 -4.4 1,783,228 - pps b1702 0.20 -5.0 1,783,228 - pps b1702 0.20 -1.0 1,783,342 - pps b1702 0.25 -5.9 1,783,681 - pps b1702 0.39 -3.6 1,783,681 - pps b1702 0.39 -3.9 1,783,852 + pps b1702 0.46 -5.5 1,783,852 + pps b1702 0.46 -5.9 1,783,992 + pps b1702 0.52 -5.1 1,784,093 - pps b1702 0.56 -5.2 1,784,142 + pps b1702 0.58 -2.5 1,784,212 + pps b1702 0.61 -2.5 1,784,322 - pps b1702 0.66 -3.6 1,784,324 - pps b1702 0.66 -1.7 1,784,418 - -1.8 1,784,432 - pps b1702 0.70 -4.3 1,784,446 - pps b1702 0.71 -4.6 1,784,446 - pps b1702 0.71 -3.8 1,784,455 - pps b1702 0.71 -3.4 1,784,472 - pps b1702 0.72 -3.3 1,784,598 + pps b1702 0.77 -3.4 1,784,610 + pps b1702 0.78 -2.9 1,784,832 + pps b1702 0.87 -3.9 1,784,832 + pps b1702 0.87 -4.7 1,784,954 + -5.1 1,785,015 + -1.6 1,785,056 + -3.8 1,785,076 + -3.4 1,785,101 + -2.1 1,785,101 + +2.1 1,785,111 + -1.1 1,785,268 + +0.2 1,785,268 + -1.6 1,785,399 - +1.0 1,785,739 + ydiA b1703 0.32 -0.1 1,785,765 + ydiA b1703 0.35 -1.4 1,785,814 - ydiA b1703 0.41 -0.8 1,785,826 - ydiA b1703 0.43 -0.6 1,785,827 - ydiA b1703 0.43 -0.3 1,785,827 - ydiA b1703 0.43 -1.1 1,785,918 + ydiA b1703 0.54 -0.3 1,785,918 + ydiA b1703 0.54 -1.2
Or see this region's nucleotide sequence