Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rspB and rspA are separated by 11 nucleotides rspA and ynfA are separated by 205 nucleotides ynfA and ynfB are separated by 134 nucleotides
b1580: rspB - predicted oxidoreductase, Zn-dependent and NAD(P)-binding (NCBI), at 1,650,920 to 1,651,939
rspB
b1581: rspA - predicted dehydratase (NCBI), at 1,651,951 to 1,653,165
rspA
b1582: ynfA - hypothetical protein (NCBI), at 1,653,371 to 1,653,697
ynfA
b1583: ynfB - hypothetical protein (NCBI), at 1,653,832 to 1,654,173
ynfB
Position (kb)
1651
1652
1653
1654 Strain fitness (log2 ratio)
-2
-1
0
1 at 1650.984 kb on - strand at 1651.150 kb on + strand, within rspB at 1651.158 kb on - strand, within rspB at 1651.163 kb on - strand, within rspB at 1651.193 kb on + strand, within rspB at 1651.211 kb on + strand, within rspB at 1651.211 kb on + strand, within rspB at 1651.219 kb on - strand, within rspB at 1651.609 kb on + strand, within rspB at 1651.618 kb on - strand, within rspB at 1651.667 kb on - strand, within rspB at 1651.677 kb on - strand, within rspB at 1651.728 kb on + strand, within rspB at 1651.728 kb on + strand, within rspB at 1651.728 kb on + strand, within rspB at 1651.728 kb on - strand, within rspB at 1651.735 kb on - strand, within rspB at 1651.740 kb on + strand, within rspB at 1651.802 kb on - strand, within rspB at 1651.859 kb on + strand at 1651.974 kb on + strand at 1652.048 kb on - strand at 1652.060 kb on - strand at 1652.070 kb on + strand at 1652.070 kb on + strand at 1652.237 kb on - strand, within rspA at 1652.628 kb on - strand, within rspA at 1652.940 kb on + strand, within rspA at 1653.008 kb on + strand, within rspA at 1653.070 kb on + strand at 1653.070 kb on + strand at 1653.166 kb on - strand at 1653.461 kb on + strand, within ynfA at 1653.557 kb on - strand, within ynfA at 1653.632 kb on + strand, within ynfA at 1653.757 kb on + strand at 1653.792 kb on + strand at 1653.800 kb on - strand at 1653.869 kb on + strand, within ynfB at 1653.869 kb on + strand at 1653.881 kb on + strand, within ynfB at 1654.127 kb on - strand, within ynfB at 1654.136 kb on + strand, within ynfB at 1654.144 kb on - strand at 1654.144 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,650,984 - +0.1 1,651,150 + rspB b1580 0.23 -0.8 1,651,158 - rspB b1580 0.23 -1.6 1,651,163 - rspB b1580 0.24 +0.5 1,651,193 + rspB b1580 0.27 +0.4 1,651,211 + rspB b1580 0.29 +1.0 1,651,211 + rspB b1580 0.29 +0.3 1,651,219 - rspB b1580 0.29 -1.5 1,651,609 + rspB b1580 0.68 +1.4 1,651,618 - rspB b1580 0.68 -1.6 1,651,667 - rspB b1580 0.73 -0.6 1,651,677 - rspB b1580 0.74 +0.1 1,651,728 + rspB b1580 0.79 +0.6 1,651,728 + rspB b1580 0.79 -2.6 1,651,728 + rspB b1580 0.79 -1.2 1,651,728 - rspB b1580 0.79 -0.7 1,651,735 - rspB b1580 0.80 -1.1 1,651,740 + rspB b1580 0.80 -0.2 1,651,802 - rspB b1580 0.86 -0.6 1,651,859 + +1.7 1,651,974 + +0.4 1,652,048 - +0.4 1,652,060 - -0.1 1,652,070 + +0.0 1,652,070 + +0.2 1,652,237 - rspA b1581 0.24 +0.2 1,652,628 - rspA b1581 0.56 +0.7 1,652,940 + rspA b1581 0.81 +1.2 1,653,008 + rspA b1581 0.87 -1.8 1,653,070 + +0.7 1,653,070 + -2.3 1,653,166 - -1.8 1,653,461 + ynfA b1582 0.28 -0.4 1,653,557 - ynfA b1582 0.57 +0.7 1,653,632 + ynfA b1582 0.80 -0.4 1,653,757 + -0.2 1,653,792 + +1.2 1,653,800 - +0.8 1,653,869 + ynfB b1583 0.11 -2.4 1,653,869 + +0.4 1,653,881 + ynfB b1583 0.14 +0.6 1,654,127 - ynfB b1583 0.86 +0.8 1,654,136 + ynfB b1583 0.89 +0.3 1,654,144 - -0.9 1,654,144 - -1.9
Or see this region's nucleotide sequence