Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydeI and ydeJ are separated by 275 nucleotides ydeJ and dcp are separated by 43 nucleotides dcp and ydfG are separated by 136 nucleotides ydfG and ydfH are separated by 88 nucleotides
b1536: ydeI - hypothetical protein (NCBI), at 1,622,129 to 1,622,521
ydeI
b1537: ydeJ - competence damage-inducible protein A (NCBI), at 1,622,797 to 1,623,315
ydeJ
b1538: dcp - dipeptidyl carboxypeptidase II (NCBI), at 1,623,359 to 1,625,404
dcp
b1539: ydfG - L-allo-threonine dehydrogenase, NAD(P)-binding (NCBI), at 1,625,541 to 1,626,287
ydfG
b1540: ydfH - predicted DNA-binding transcriptional regulator (NCBI), at 1,626,376 to 1,627,062
ydfH
Position (kb)
1623
1624
1625
1626 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1622.405 kb on - strand, within ydeI at 1622.405 kb on - strand, within ydeI at 1622.409 kb on + strand, within ydeI at 1622.409 kb on + strand, within ydeI at 1622.420 kb on + strand, within ydeI at 1622.420 kb on + strand, within ydeI at 1622.420 kb on + strand, within ydeI at 1622.471 kb on - strand, within ydeI at 1622.527 kb on + strand at 1622.774 kb on - strand at 1622.834 kb on + strand at 1622.835 kb on - strand at 1622.838 kb on - strand at 1622.868 kb on + strand, within ydeJ at 1622.868 kb on + strand, within ydeJ at 1622.934 kb on + strand, within ydeJ at 1622.983 kb on + strand, within ydeJ at 1622.987 kb on + strand, within ydeJ at 1623.043 kb on + strand, within ydeJ at 1623.097 kb on + strand, within ydeJ at 1623.097 kb on + strand, within ydeJ at 1623.170 kb on + strand, within ydeJ at 1623.211 kb on + strand, within ydeJ at 1623.259 kb on + strand, within ydeJ at 1623.259 kb on + strand, within ydeJ at 1623.297 kb on + strand at 1623.297 kb on + strand at 1623.309 kb on - strand at 1623.309 kb on - strand at 1623.411 kb on - strand at 1623.449 kb on + strand at 1623.449 kb on + strand at 1623.501 kb on + strand at 1623.519 kb on - strand at 1623.739 kb on + strand, within dcp at 1623.739 kb on + strand, within dcp at 1623.752 kb on - strand, within dcp at 1623.772 kb on - strand, within dcp at 1623.772 kb on - strand, within dcp at 1623.801 kb on + strand, within dcp at 1623.844 kb on - strand, within dcp at 1623.892 kb on - strand, within dcp at 1623.951 kb on + strand, within dcp at 1624.060 kb on - strand, within dcp at 1624.089 kb on + strand, within dcp at 1624.237 kb on - strand, within dcp at 1624.297 kb on - strand, within dcp at 1624.987 kb on + strand, within dcp at 1624.992 kb on + strand, within dcp at 1625.044 kb on + strand, within dcp at 1625.044 kb on + strand, within dcp at 1625.169 kb on - strand, within dcp at 1625.380 kb on + strand at 1625.442 kb on - strand at 1625.443 kb on - strand at 1625.443 kb on - strand at 1625.681 kb on + strand, within ydfG at 1625.871 kb on + strand, within ydfG at 1625.871 kb on + strand, within ydfG at 1626.043 kb on - strand, within ydfG
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,622,405 - ydeI b1536 0.70 +0.1 1,622,405 - ydeI b1536 0.70 -1.6 1,622,409 + ydeI b1536 0.71 +0.5 1,622,409 + ydeI b1536 0.71 +2.1 1,622,420 + ydeI b1536 0.74 -1.9 1,622,420 + ydeI b1536 0.74 +4.1 1,622,420 + ydeI b1536 0.74 +2.1 1,622,471 - ydeI b1536 0.87 -0.2 1,622,527 + +2.1 1,622,774 - +0.5 1,622,834 + +2.1 1,622,835 - -1.9 1,622,838 - -0.5 1,622,868 + ydeJ b1537 0.14 +2.1 1,622,868 + ydeJ b1537 0.14 +2.1 1,622,934 + ydeJ b1537 0.26 +0.3 1,622,983 + ydeJ b1537 0.36 +2.0 1,622,987 + ydeJ b1537 0.37 +1.4 1,623,043 + ydeJ b1537 0.47 +0.1 1,623,097 + ydeJ b1537 0.58 -1.3 1,623,097 + ydeJ b1537 0.58 -0.3 1,623,170 + ydeJ b1537 0.72 +0.1 1,623,211 + ydeJ b1537 0.80 -0.3 1,623,259 + ydeJ b1537 0.89 +0.8 1,623,259 + ydeJ b1537 0.89 +0.0 1,623,297 + -1.0 1,623,297 + -0.2 1,623,309 - -0.6 1,623,309 - -0.8 1,623,411 - +0.2 1,623,449 + +0.7 1,623,449 + -1.8 1,623,501 + -0.4 1,623,519 - -0.7 1,623,739 + dcp b1538 0.19 +2.1 1,623,739 + dcp b1538 0.19 +0.6 1,623,752 - dcp b1538 0.19 +0.6 1,623,772 - dcp b1538 0.20 +0.5 1,623,772 - dcp b1538 0.20 +0.2 1,623,801 + dcp b1538 0.22 +0.3 1,623,844 - dcp b1538 0.24 -0.3 1,623,892 - dcp b1538 0.26 +0.3 1,623,951 + dcp b1538 0.29 -3.0 1,624,060 - dcp b1538 0.34 +0.5 1,624,089 + dcp b1538 0.36 +2.1 1,624,237 - dcp b1538 0.43 +0.5 1,624,297 - dcp b1538 0.46 +1.4 1,624,987 + dcp b1538 0.80 -0.3 1,624,992 + dcp b1538 0.80 +0.1 1,625,044 + dcp b1538 0.82 -1.5 1,625,044 + dcp b1538 0.82 +1.0 1,625,169 - dcp b1538 0.88 +0.3 1,625,380 + -1.5 1,625,442 - -0.2 1,625,443 - +0.7 1,625,443 - -0.9 1,625,681 + ydfG b1539 0.19 -0.7 1,625,871 + ydfG b1539 0.44 -1.3 1,625,871 + ydfG b1539 0.44 -1.9 1,626,043 - ydfG b1539 0.67 +0.2
Or see this region's nucleotide sequence