Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydeD and ydeE are separated by 194 nucleotides ydeE and yneM are separated by 126 nucleotides yneM and mgrR are separated by 75 nucleotides mgrR and ydeH are separated by 45 nucleotides
b1533: ydeD - orf, hypothetical protein (VIMSS), at 1,618,262 to 1,619,161
ydeD
b1534: ydeE - predicted transporter (NCBI), at 1,619,356 to 1,620,543
ydeE
b4599: yneM - hypothetical protein (RefSeq), at 1,620,670 to 1,620,765
yneM
b4698: mgrR - sRNA antisense regulator down regulates YgdQ and EptB, PhoPQ-regulated, Hfq-dependent; affects sensitivity to antimicrobial peptides (RefSeq), at 1,620,841 to 1,620,938
mgrR
b1535: ydeH - hypothetical protein (NCBI), at 1,620,984 to 1,621,874
ydeH
Position (kb)
1619
1620
1621 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1618.521 kb on - strand, within ydeD at 1618.521 kb on - strand, within ydeD at 1618.653 kb on + strand, within ydeD at 1618.995 kb on + strand, within ydeD at 1618.995 kb on + strand, within ydeD at 1619.282 kb on - strand at 1619.420 kb on + strand at 1619.420 kb on + strand at 1619.466 kb on + strand at 1619.668 kb on + strand, within ydeE at 1619.759 kb on + strand, within ydeE at 1619.768 kb on + strand, within ydeE at 1619.780 kb on + strand, within ydeE at 1619.872 kb on - strand, within ydeE at 1619.929 kb on + strand, within ydeE at 1619.929 kb on + strand, within ydeE at 1620.034 kb on - strand, within ydeE at 1620.095 kb on + strand, within ydeE at 1620.183 kb on + strand, within ydeE at 1620.302 kb on - strand, within ydeE at 1620.316 kb on + strand, within ydeE at 1620.372 kb on + strand, within ydeE at 1620.372 kb on + strand, within ydeE at 1620.421 kb on + strand, within ydeE at 1620.424 kb on - strand, within ydeE at 1620.424 kb on - strand, within ydeE at 1620.435 kb on + strand at 1620.435 kb on + strand at 1620.503 kb on + strand at 1620.522 kb on + strand at 1620.581 kb on + strand at 1620.638 kb on + strand at 1620.638 kb on + strand at 1620.638 kb on + strand at 1620.651 kb on + strand at 1620.772 kb on - strand at 1620.779 kb on + strand at 1620.784 kb on + strand at 1620.784 kb on + strand at 1620.800 kb on + strand at 1620.800 kb on + strand at 1620.807 kb on + strand at 1620.812 kb on + strand at 1620.812 kb on + strand at 1620.874 kb on - strand, within mgrR at 1620.874 kb on - strand, within mgrR at 1620.879 kb on - strand, within mgrR at 1620.926 kb on - strand, within mgrR at 1620.946 kb on - strand at 1620.978 kb on - strand at 1620.990 kb on - strand at 1621.058 kb on - strand at 1621.058 kb on - strand at 1621.084 kb on - strand, within ydeH at 1621.084 kb on - strand, within ydeH at 1621.089 kb on - strand, within ydeH at 1621.094 kb on - strand, within ydeH at 1621.094 kb on - strand, within ydeH at 1621.103 kb on + strand, within ydeH at 1621.121 kb on - strand, within ydeH at 1621.207 kb on - strand, within ydeH at 1621.207 kb on - strand, within ydeH at 1621.215 kb on - strand, within ydeH at 1621.304 kb on - strand, within ydeH at 1621.307 kb on + strand, within ydeH at 1621.315 kb on - strand, within ydeH at 1621.317 kb on + strand, within ydeH at 1621.323 kb on - strand, within ydeH at 1621.325 kb on - strand, within ydeH at 1621.416 kb on - strand, within ydeH at 1621.439 kb on - strand, within ydeH at 1621.459 kb on - strand, within ydeH
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,618,521 - ydeD b1533 0.29 +0.1 1,618,521 - ydeD b1533 0.29 -2.3 1,618,653 + ydeD b1533 0.43 +0.8 1,618,995 + ydeD b1533 0.81 -3.1 1,618,995 + ydeD b1533 0.81 +0.2 1,619,282 - +0.7 1,619,420 + +1.2 1,619,420 + +0.4 1,619,466 + +0.3 1,619,668 + ydeE b1534 0.26 +2.4 1,619,759 + ydeE b1534 0.34 +1.2 1,619,768 + ydeE b1534 0.35 +0.3 1,619,780 + ydeE b1534 0.36 +0.5 1,619,872 - ydeE b1534 0.43 -0.3 1,619,929 + ydeE b1534 0.48 +1.0 1,619,929 + ydeE b1534 0.48 +0.8 1,620,034 - ydeE b1534 0.57 -0.8 1,620,095 + ydeE b1534 0.62 +0.1 1,620,183 + ydeE b1534 0.70 +0.2 1,620,302 - ydeE b1534 0.80 +2.1 1,620,316 + ydeE b1534 0.81 -2.7 1,620,372 + ydeE b1534 0.86 +0.4 1,620,372 + ydeE b1534 0.86 +0.3 1,620,421 + ydeE b1534 0.90 +0.2 1,620,424 - ydeE b1534 0.90 -3.4 1,620,424 - ydeE b1534 0.90 +1.9 1,620,435 + +0.3 1,620,435 + -0.2 1,620,503 + +0.5 1,620,522 + +0.3 1,620,581 + -1.3 1,620,638 + +0.1 1,620,638 + -0.8 1,620,638 + +0.4 1,620,651 + -3.5 1,620,772 - +1.1 1,620,779 + +0.9 1,620,784 + -1.5 1,620,784 + -0.5 1,620,800 + +0.0 1,620,800 + +1.6 1,620,807 + -0.3 1,620,812 + -0.1 1,620,812 + +1.1 1,620,874 - mgrR b4698 0.34 +0.2 1,620,874 - mgrR b4698 0.34 -0.7 1,620,879 - mgrR b4698 0.39 +0.5 1,620,926 - mgrR b4698 0.87 -0.1 1,620,946 - +0.1 1,620,978 - -0.1 1,620,990 - -1.1 1,621,058 - -0.9 1,621,058 - +1.3 1,621,084 - ydeH b1535 0.11 +0.4 1,621,084 - ydeH b1535 0.11 +0.3 1,621,089 - ydeH b1535 0.12 -0.4 1,621,094 - ydeH b1535 0.12 +0.1 1,621,094 - ydeH b1535 0.12 +0.1 1,621,103 + ydeH b1535 0.13 -1.0 1,621,121 - ydeH b1535 0.15 +0.7 1,621,207 - ydeH b1535 0.25 +0.5 1,621,207 - ydeH b1535 0.25 +1.8 1,621,215 - ydeH b1535 0.26 +0.1 1,621,304 - ydeH b1535 0.36 +0.4 1,621,307 + ydeH b1535 0.36 +2.1 1,621,315 - ydeH b1535 0.37 -1.8 1,621,317 + ydeH b1535 0.37 +2.1 1,621,323 - ydeH b1535 0.38 +0.5 1,621,325 - ydeH b1535 0.38 +0.5 1,621,416 - ydeH b1535 0.48 -0.7 1,621,439 - ydeH b1535 0.51 +1.0 1,621,459 - ydeH b1535 0.53 -1.0
Or see this region's nucleotide sequence