Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt maoC and paaA are separated by 284 nucleotides paaA and paaB are separated by 11 nucleotides paaB and paaC are separated by 8 nucleotides
b1387: maoC - fused aldehyde dehydrogenase/enoyl-CoA hydratase (NCBI), at 1,449,621 to 1,451,666
maoC
b1388: paaA - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI), at 1,451,951 to 1,452,880
paaA
b1389: paaB - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI), at 1,452,892 to 1,453,179
paaB
b1390: paaC - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI), at 1,453,188 to 1,453,934
paaC
Position (kb)
1451
1452
1453 Strain fitness (log2 ratio)
-1
0
1 at 1451.006 kb on - strand, within maoC at 1451.232 kb on + strand, within maoC at 1451.232 kb on + strand, within maoC at 1451.305 kb on + strand, within maoC at 1451.595 kb on + strand at 1451.984 kb on + strand at 1451.984 kb on + strand at 1452.190 kb on - strand, within paaA at 1452.425 kb on - strand, within paaA at 1452.529 kb on - strand, within paaA at 1452.529 kb on - strand, within paaA at 1452.655 kb on + strand, within paaA at 1453.221 kb on + strand at 1453.221 kb on + strand at 1453.483 kb on - strand, within paaC at 1453.635 kb on - strand, within paaC at 1453.641 kb on + strand, within paaC at 1453.689 kb on + strand, within paaC at 1453.840 kb on - strand, within paaC
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,451,006 - maoC b1387 0.68 +0.9 1,451,232 + maoC b1387 0.79 +0.0 1,451,232 + maoC b1387 0.79 -0.0 1,451,305 + maoC b1387 0.82 +0.6 1,451,595 + -0.2 1,451,984 + -0.0 1,451,984 + +0.5 1,452,190 - paaA b1388 0.26 +0.7 1,452,425 - paaA b1388 0.51 +0.4 1,452,529 - paaA b1388 0.62 +0.3 1,452,529 - paaA b1388 0.62 +0.2 1,452,655 + paaA b1388 0.76 +0.3 1,453,221 + -0.3 1,453,221 + +0.4 1,453,483 - paaC b1390 0.39 -0.4 1,453,635 - paaC b1390 0.60 -0.1 1,453,641 + paaC b1390 0.61 +0.9 1,453,689 + paaC b1390 0.67 -0.6 1,453,840 - paaC b1390 0.87 -0.0
Or see this region's nucleotide sequence