Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ompN and micC are separated by 227 nucleotides micC and ydbK are separated by 30 nucleotides ydbK and ydbJ are separated by 273 nucleotides ydbJ and hslJ overlap by 4 nucleotides
b1377: ompN - outer membrane pore protein N, non-specific (NCBI), at 1,433,784 to 1,434,917
ompN
b4427: micC - regulatory sRNA (NCBI), at 1,435,145 to 1,435,253
micC
b1378: ydbK - fused predicted pyruvate-flavodoxin oxidoreductase: conserved protein/conserved protein/FeS binding protein (NCBI), at 1,435,284 to 1,438,808
ydbK
b4529: ydbJ - hypothetical protein (NCBI), at 1,439,082 to 1,439,348
ydbJ
b1379: hslJ - heat-inducible protein (NCBI), at 1,439,345 to 1,439,767
hslJ
Position (kb)
1435
1436
1437
1438
1439 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1434.295 kb on + strand, within ompN at 1434.347 kb on - strand, within ompN at 1434.357 kb on - strand, within ompN at 1434.397 kb on + strand, within ompN at 1434.523 kb on + strand, within ompN at 1434.523 kb on + strand, within ompN at 1434.570 kb on + strand, within ompN at 1434.633 kb on + strand, within ompN at 1434.656 kb on + strand, within ompN at 1434.753 kb on + strand, within ompN at 1434.808 kb on + strand at 1435.326 kb on - strand at 1435.374 kb on - strand at 1435.571 kb on + strand at 1435.584 kb on + strand at 1435.584 kb on + strand at 1435.609 kb on - strand at 1435.887 kb on - strand, within ydbK at 1435.887 kb on - strand, within ydbK at 1435.915 kb on - strand, within ydbK at 1435.915 kb on - strand, within ydbK at 1436.058 kb on + strand, within ydbK at 1436.279 kb on + strand, within ydbK at 1436.310 kb on - strand, within ydbK at 1436.510 kb on - strand, within ydbK at 1436.561 kb on + strand, within ydbK at 1436.643 kb on + strand, within ydbK at 1436.748 kb on - strand, within ydbK at 1436.857 kb on - strand, within ydbK at 1437.029 kb on - strand, within ydbK at 1437.120 kb on + strand, within ydbK at 1437.120 kb on + strand, within ydbK at 1437.254 kb on - strand, within ydbK at 1437.369 kb on + strand, within ydbK at 1437.799 kb on - strand, within ydbK at 1437.832 kb on + strand, within ydbK at 1438.460 kb on + strand at 1438.501 kb on + strand at 1438.540 kb on - strand at 1438.709 kb on - strand at 1438.815 kb on + strand at 1438.823 kb on - strand at 1438.926 kb on - strand at 1438.926 kb on - strand at 1438.997 kb on + strand at 1438.998 kb on - strand at 1439.084 kb on - strand at 1439.084 kb on - strand at 1439.137 kb on - strand, within ydbJ at 1439.365 kb on + strand at 1439.401 kb on - strand, within hslJ at 1439.561 kb on - strand, within hslJ at 1439.561 kb on - strand, within hslJ at 1439.591 kb on + strand, within hslJ at 1439.591 kb on + strand, within hslJ at 1439.654 kb on + strand, within hslJ
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,434,295 + ompN b1377 0.45 -0.3 1,434,347 - ompN b1377 0.50 -1.0 1,434,357 - ompN b1377 0.51 -0.7 1,434,397 + ompN b1377 0.54 -1.9 1,434,523 + ompN b1377 0.65 -0.2 1,434,523 + ompN b1377 0.65 -1.1 1,434,570 + ompN b1377 0.69 -0.2 1,434,633 + ompN b1377 0.75 +2.1 1,434,656 + ompN b1377 0.77 +1.7 1,434,753 + ompN b1377 0.85 +2.1 1,434,808 + +2.1 1,435,326 - -0.5 1,435,374 - +0.7 1,435,571 + -0.2 1,435,584 + -0.5 1,435,584 + +1.6 1,435,609 - +0.5 1,435,887 - ydbK b1378 0.17 +1.2 1,435,887 - ydbK b1378 0.17 -0.1 1,435,915 - ydbK b1378 0.18 +0.2 1,435,915 - ydbK b1378 0.18 +0.4 1,436,058 + ydbK b1378 0.22 -1.7 1,436,279 + ydbK b1378 0.28 -0.6 1,436,310 - ydbK b1378 0.29 -1.6 1,436,510 - ydbK b1378 0.35 +0.4 1,436,561 + ydbK b1378 0.36 -1.5 1,436,643 + ydbK b1378 0.39 -2.2 1,436,748 - ydbK b1378 0.42 +0.7 1,436,857 - ydbK b1378 0.45 +0.5 1,437,029 - ydbK b1378 0.50 +1.2 1,437,120 + ydbK b1378 0.52 +1.6 1,437,120 + ydbK b1378 0.52 -0.3 1,437,254 - ydbK b1378 0.56 +0.6 1,437,369 + ydbK b1378 0.59 +0.2 1,437,799 - ydbK b1378 0.71 -0.6 1,437,832 + ydbK b1378 0.72 -1.4 1,438,460 + +0.5 1,438,501 + -1.9 1,438,540 - +0.3 1,438,709 - -0.0 1,438,815 + +0.6 1,438,823 - +0.1 1,438,926 - +0.4 1,438,926 - +0.7 1,438,997 + +0.1 1,438,998 - -0.8 1,439,084 - +0.1 1,439,084 - +0.6 1,439,137 - ydbJ b4529 0.21 +0.2 1,439,365 + -0.1 1,439,401 - hslJ b1379 0.13 +0.0 1,439,561 - hslJ b1379 0.51 -0.0 1,439,561 - hslJ b1379 0.51 +0.2 1,439,591 + hslJ b1379 0.58 +0.2 1,439,591 + hslJ b1379 0.58 +0.8 1,439,654 + hslJ b1379 0.73 -0.0
Or see this region's nucleotide sequence