Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycgB and dadA are separated by 329 nucleotides dadA and dadX are separated by 9 nucleotides
b1188: ycgB - ycgB component of nitrogen-related signalling system (of yeaGH-ycgB) (from data) , at 1,234,932 to 1,236,464
ycgB
b1189: dadA - D-amino acid dehydrogenase (quinone) DadA (EC 1.4.5.1) (from data) , at 1,236,794 to 1,238,092
dadA
b1190: dadX - alanine racemase (NCBI), at 1,238,102 to 1,239,172
dadX
Position (kb)
1236
1237
1238
1239 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1235.815 kb on - strand, within ycgB at 1235.815 kb on - strand, within ycgB at 1236.122 kb on + strand, within ycgB at 1236.187 kb on - strand, within ycgB at 1236.339 kb on - strand at 1236.345 kb on - strand at 1236.374 kb on + strand at 1236.374 kb on + strand at 1236.414 kb on - strand at 1236.456 kb on - strand at 1236.788 kb on - strand at 1236.788 kb on - strand at 1236.803 kb on + strand at 1236.924 kb on - strand, within dadA at 1237.037 kb on + strand, within dadA at 1237.040 kb on - strand, within dadA at 1237.146 kb on + strand, within dadA at 1237.180 kb on + strand, within dadA at 1237.213 kb on + strand, within dadA at 1237.213 kb on + strand, within dadA at 1237.218 kb on + strand, within dadA at 1237.507 kb on + strand, within dadA at 1237.545 kb on - strand, within dadA at 1237.545 kb on - strand, within dadA at 1237.803 kb on + strand, within dadA at 1237.803 kb on + strand, within dadA at 1238.119 kb on + strand at 1238.119 kb on + strand at 1238.234 kb on - strand, within dadX at 1238.234 kb on - strand, within dadX at 1238.275 kb on + strand, within dadX at 1238.275 kb on + strand, within dadX at 1238.292 kb on + strand, within dadX at 1238.803 kb on + strand, within dadX at 1238.803 kb on + strand, within dadX at 1238.855 kb on + strand, within dadX at 1238.874 kb on - strand, within dadX at 1238.920 kb on - strand, within dadX at 1238.920 kb on - strand, within dadX
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,235,815 - ycgB b1188 0.58 -0.8 1,235,815 - ycgB b1188 0.58 +0.5 1,236,122 + ycgB b1188 0.78 +0.3 1,236,187 - ycgB b1188 0.82 -0.6 1,236,339 - -0.5 1,236,345 - +0.1 1,236,374 + -0.5 1,236,374 + -1.6 1,236,414 - -1.4 1,236,456 - -0.1 1,236,788 - -3.0 1,236,788 - -0.8 1,236,803 + -1.4 1,236,924 - dadA b1189 0.10 +0.7 1,237,037 + dadA b1189 0.19 +0.4 1,237,040 - dadA b1189 0.19 +0.8 1,237,146 + dadA b1189 0.27 +0.1 1,237,180 + dadA b1189 0.30 +0.4 1,237,213 + dadA b1189 0.32 -2.1 1,237,213 + dadA b1189 0.32 +0.7 1,237,218 + dadA b1189 0.33 -0.5 1,237,507 + dadA b1189 0.55 -1.9 1,237,545 - dadA b1189 0.58 -0.1 1,237,545 - dadA b1189 0.58 +0.9 1,237,803 + dadA b1189 0.78 -0.1 1,237,803 + dadA b1189 0.78 +0.4 1,238,119 + +0.8 1,238,119 + +0.3 1,238,234 - dadX b1190 0.12 -0.1 1,238,234 - dadX b1190 0.12 -1.5 1,238,275 + dadX b1190 0.16 +0.0 1,238,275 + dadX b1190 0.16 +0.4 1,238,292 + dadX b1190 0.18 +0.3 1,238,803 + dadX b1190 0.65 +0.2 1,238,803 + dadX b1190 0.65 -0.0 1,238,855 + dadX b1190 0.70 +0.6 1,238,874 - dadX b1190 0.72 +1.0 1,238,920 - dadX b1190 0.76 -2.0 1,238,920 - dadX b1190 0.76 -0.8
Or see this region's nucleotide sequence