Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafK and yafQ are separated by 155 nucleotides yafQ and dinJ are separated by 2 nucleotides dinJ and yafL are separated by 209 nucleotides yafL and yafM are separated by 175 nucleotides yafM and lfhA are separated by 223 nucleotides
b0224: yafK - hypothetical protein (NCBI), at 245,065 to 245,805
yafK
b0225: yafQ - predicted toxin of the YafQ-DinJ toxin-antitoxin system (NCBI), at 245,961 to 246,239
yafQ
b0226: dinJ - predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI), at 246,242 to 246,502
dinJ
b0227: yafL - predicted lipoprotein and C40 family peptidase (NCBI), at 246,712 to 247,461
yafL
b0228: yafM - hypothetical protein (NCBI), at 247,637 to 248,134
yafM
b0229: lfhA - flagellar biosynthesis (VIMSS), at 248,358 to 250,070
lfhA
Position (kb)
246
247
248 Strain fitness (log2 ratio)
-2
-1
0
1 at 245.735 kb on - strand at 245.743 kb on - strand at 245.743 kb on - strand at 245.837 kb on + strand at 245.928 kb on - strand at 245.928 kb on - strand at 245.964 kb on + strand at 245.964 kb on + strand at 245.971 kb on - strand at 246.048 kb on + strand, within yafQ at 246.048 kb on + strand, within yafQ at 246.048 kb on + strand, within yafQ at 246.086 kb on - strand, within yafQ at 246.086 kb on - strand, within yafQ at 246.086 kb on - strand, within yafQ at 246.086 kb on - strand, within yafQ at 246.097 kb on - strand, within yafQ at 246.294 kb on - strand, within dinJ at 246.295 kb on - strand, within dinJ at 246.304 kb on - strand, within dinJ at 246.330 kb on - strand, within dinJ at 246.338 kb on - strand, within dinJ at 246.338 kb on - strand, within dinJ at 246.411 kb on + strand, within dinJ at 246.411 kb on + strand, within dinJ at 246.742 kb on - strand at 246.821 kb on - strand, within yafL at 246.854 kb on + strand, within yafL at 246.859 kb on + strand, within yafL at 247.129 kb on - strand, within yafL at 247.129 kb on - strand, within yafL at 247.227 kb on - strand, within yafL at 247.227 kb on - strand, within yafL at 247.288 kb on + strand, within yafL at 247.312 kb on - strand, within yafL at 247.409 kb on - strand at 247.493 kb on + strand at 247.502 kb on - strand at 247.729 kb on + strand, within yafM at 247.786 kb on - strand, within yafM at 247.786 kb on - strand, within yafM at 247.797 kb on - strand, within yafM at 247.824 kb on - strand, within yafM at 247.831 kb on - strand, within yafM at 248.035 kb on + strand, within yafM at 248.067 kb on + strand, within yafM at 248.185 kb on - strand at 248.185 kb on - strand at 248.336 kb on + strand at 248.337 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 245,735 - -0.3 245,743 - +1.2 245,743 - -0.3 245,837 + -0.0 245,928 - -1.2 245,928 - +0.6 245,964 + +0.6 245,964 + -0.7 245,971 - -1.5 246,048 + yafQ b0225 0.31 +0.7 246,048 + yafQ b0225 0.31 +0.3 246,048 + yafQ b0225 0.31 -0.8 246,086 - yafQ b0225 0.45 -0.8 246,086 - yafQ b0225 0.45 +0.8 246,086 - yafQ b0225 0.45 +0.5 246,086 - yafQ b0225 0.45 +1.4 246,097 - yafQ b0225 0.49 +0.3 246,294 - dinJ b0226 0.20 +1.0 246,295 - dinJ b0226 0.20 -0.9 246,304 - dinJ b0226 0.24 -0.3 246,330 - dinJ b0226 0.34 -1.8 246,338 - dinJ b0226 0.37 -0.5 246,338 - dinJ b0226 0.37 -0.1 246,411 + dinJ b0226 0.65 -0.5 246,411 + dinJ b0226 0.65 -0.7 246,742 - -0.6 246,821 - yafL b0227 0.15 +0.4 246,854 + yafL b0227 0.19 +0.6 246,859 + yafL b0227 0.20 +0.0 247,129 - yafL b0227 0.56 +0.2 247,129 - yafL b0227 0.56 +1.3 247,227 - yafL b0227 0.69 +0.5 247,227 - yafL b0227 0.69 -0.1 247,288 + yafL b0227 0.77 +0.1 247,312 - yafL b0227 0.80 +0.5 247,409 - -1.0 247,493 + -0.4 247,502 - +0.1 247,729 + yafM b0228 0.18 -0.7 247,786 - yafM b0228 0.30 -1.3 247,786 - yafM b0228 0.30 +0.7 247,797 - yafM b0228 0.32 +0.6 247,824 - yafM b0228 0.38 +0.1 247,831 - yafM b0228 0.39 -0.2 248,035 + yafM b0228 0.80 +0.1 248,067 + yafM b0228 0.86 -0.4 248,185 - -0.0 248,185 - +0.2 248,336 + -1.4 248,337 - +0.3
Or see this region's nucleotide sequence