Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisB and hisH overlap by 1 nucleotides hisH and hisA overlap by 1 nucleotides hisA and hisF overlap by 19 nucleotides
b2022: hisB - imidazoleglycerolphosphate dehydratase and histidinol-phosphate phosphatase (VIMSS), at 2,091,492 to 2,092,559
hisB
b2023: hisH - imidazole glycerol phosphate synthase subunit HisH (NCBI), at 2,092,559 to 2,093,149
hisH
b2024: hisA - N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS), at 2,093,149 to 2,093,886
hisA
b2025: hisF - imidazole glycerol phosphate synthase subunit HisF (NCBI), at 2,093,868 to 2,094,644
hisF
Position (kb)
2092
2093
2094 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2091.593 kb on - strand at 2091.593 kb on - strand at 2091.619 kb on - strand, within hisB at 2091.659 kb on + strand, within hisB at 2091.985 kb on - strand, within hisB at 2092.013 kb on - strand, within hisB at 2092.013 kb on - strand, within hisB at 2092.070 kb on + strand, within hisB at 2092.080 kb on + strand, within hisB at 2092.114 kb on - strand, within hisB at 2092.160 kb on + strand, within hisB at 2092.171 kb on - strand, within hisB at 2092.193 kb on - strand, within hisB at 2092.193 kb on - strand, within hisB at 2092.355 kb on + strand, within hisB at 2092.355 kb on + strand, within hisB at 2092.359 kb on - strand, within hisB at 2092.406 kb on + strand, within hisB at 2092.406 kb on + strand, within hisB at 2092.412 kb on + strand, within hisB at 2092.412 kb on + strand, within hisB at 2092.464 kb on + strand at 2092.533 kb on - strand at 2092.549 kb on - strand at 2092.752 kb on - strand, within hisH at 2092.772 kb on - strand, within hisH at 2092.907 kb on + strand, within hisH at 2093.026 kb on + strand, within hisH at 2093.026 kb on + strand, within hisH at 2093.071 kb on + strand, within hisH at 2093.071 kb on + strand, within hisH at 2093.079 kb on - strand, within hisH at 2093.079 kb on - strand, within hisH at 2093.079 kb on - strand, within hisH at 2093.079 kb on - strand, within hisH at 2093.094 kb on - strand at 2093.183 kb on + strand at 2093.183 kb on + strand at 2093.454 kb on - strand, within hisA at 2093.510 kb on - strand, within hisA at 2093.818 kb on - strand at 2093.818 kb on - strand at 2093.881 kb on + strand at 2093.918 kb on + strand at 2093.978 kb on + strand, within hisF
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 2,091,593 - -2.1 2,091,593 - -2.5 2,091,619 - hisB b2022 0.12 -1.1 2,091,659 + hisB b2022 0.16 -4.9 2,091,985 - hisB b2022 0.46 -4.4 2,092,013 - hisB b2022 0.49 -1.9 2,092,013 - hisB b2022 0.49 -2.2 2,092,070 + hisB b2022 0.54 -3.9 2,092,080 + hisB b2022 0.55 -5.3 2,092,114 - hisB b2022 0.58 -2.9 2,092,160 + hisB b2022 0.63 -1.9 2,092,171 - hisB b2022 0.64 -2.8 2,092,193 - hisB b2022 0.66 -2.8 2,092,193 - hisB b2022 0.66 -3.5 2,092,355 + hisB b2022 0.81 -4.3 2,092,355 + hisB b2022 0.81 -0.6 2,092,359 - hisB b2022 0.81 -2.5 2,092,406 + hisB b2022 0.86 -2.6 2,092,406 + hisB b2022 0.86 -6.7 2,092,412 + hisB b2022 0.86 -2.8 2,092,412 + hisB b2022 0.86 -3.7 2,092,464 + -5.3 2,092,533 - -2.1 2,092,549 - -2.5 2,092,752 - hisH b2023 0.33 -2.5 2,092,772 - hisH b2023 0.36 -6.1 2,092,907 + hisH b2023 0.59 -3.6 2,093,026 + hisH b2023 0.79 -5.3 2,093,026 + hisH b2023 0.79 -4.8 2,093,071 + hisH b2023 0.87 -3.5 2,093,071 + hisH b2023 0.87 -3.9 2,093,079 - hisH b2023 0.88 -3.1 2,093,079 - hisH b2023 0.88 -4.9 2,093,079 - hisH b2023 0.88 -3.1 2,093,079 - hisH b2023 0.88 -5.3 2,093,094 - -4.3 2,093,183 + -3.0 2,093,183 + -3.7 2,093,454 - hisA b2024 0.41 -4.0 2,093,510 - hisA b2024 0.49 -2.2 2,093,818 - -1.9 2,093,818 - -2.2 2,093,881 + -4.5 2,093,918 + -4.6 2,093,978 + hisF b2025 0.14 -4.5
Or see this region's nucleotide sequence