Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisG and hisD are separated by 5 nucleotides hisD and hisC overlap by 4 nucleotides
b2019: hisG - ATP phosphoribosyltransferase (NCBI), at 2,088,216 to 2,089,115
hisG
b2020: hisD - histidinol dehydrogenase (NCBI), at 2,089,121 to 2,090,425
hisD
b2021: hisC - histidinol-phosphate aminotransferase (NCBI), at 2,090,422 to 2,091,492
hisC
Position (kb)
2089
2090
2091 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 2088.203 kb on - strand at 2088.203 kb on - strand at 2088.316 kb on + strand, within hisG at 2088.484 kb on - strand, within hisG at 2088.602 kb on + strand, within hisG at 2088.610 kb on - strand, within hisG at 2088.610 kb on - strand, within hisG at 2088.657 kb on - strand, within hisG at 2088.681 kb on - strand, within hisG at 2088.859 kb on + strand, within hisG at 2088.900 kb on + strand, within hisG at 2088.900 kb on + strand, within hisG at 2089.029 kb on + strand at 2089.104 kb on + strand at 2089.133 kb on - strand at 2089.155 kb on - strand at 2089.187 kb on - strand at 2089.234 kb on - strand at 2089.234 kb on - strand at 2089.267 kb on + strand, within hisD at 2089.326 kb on + strand, within hisD at 2089.419 kb on - strand, within hisD at 2089.504 kb on + strand, within hisD at 2089.605 kb on - strand, within hisD at 2089.755 kb on - strand, within hisD at 2089.776 kb on + strand, within hisD at 2089.844 kb on + strand, within hisD at 2089.929 kb on - strand, within hisD at 2089.929 kb on - strand, within hisD at 2090.018 kb on - strand, within hisD at 2090.018 kb on - strand, within hisD at 2090.075 kb on - strand, within hisD at 2090.124 kb on + strand, within hisD at 2090.124 kb on + strand, within hisD at 2090.132 kb on - strand, within hisD at 2090.300 kb on - strand at 2090.337 kb on - strand at 2090.419 kb on + strand at 2090.721 kb on - strand, within hisC at 2090.808 kb on - strand, within hisC at 2090.932 kb on + strand, within hisC at 2090.932 kb on + strand, within hisC at 2090.938 kb on + strand, within hisC at 2091.102 kb on + strand, within hisC at 2091.202 kb on - strand, within hisC at 2091.202 kb on - strand, within hisC at 2091.274 kb on + strand, within hisC at 2091.351 kb on + strand, within hisC
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 2,088,203 - -0.3 2,088,203 - -0.4 2,088,316 + hisG b2019 0.11 -4.6 2,088,484 - hisG b2019 0.30 -2.8 2,088,602 + hisG b2019 0.43 -5.5 2,088,610 - hisG b2019 0.44 -3.8 2,088,610 - hisG b2019 0.44 -2.4 2,088,657 - hisG b2019 0.49 -3.8 2,088,681 - hisG b2019 0.52 -2.2 2,088,859 + hisG b2019 0.71 +0.9 2,088,900 + hisG b2019 0.76 -1.2 2,088,900 + hisG b2019 0.76 -2.8 2,089,029 + -1.7 2,089,104 + -2.7 2,089,133 - -2.7 2,089,155 - -3.6 2,089,187 - -5.3 2,089,234 - -4.7 2,089,234 - -3.2 2,089,267 + hisD b2020 0.11 -5.5 2,089,326 + hisD b2020 0.16 -4.2 2,089,419 - hisD b2020 0.23 -5.3 2,089,504 + hisD b2020 0.29 -1.8 2,089,605 - hisD b2020 0.37 -2.3 2,089,755 - hisD b2020 0.49 -3.1 2,089,776 + hisD b2020 0.50 -3.7 2,089,844 + hisD b2020 0.55 -5.2 2,089,929 - hisD b2020 0.62 -2.7 2,089,929 - hisD b2020 0.62 -2.1 2,090,018 - hisD b2020 0.69 -4.4 2,090,018 - hisD b2020 0.69 -3.7 2,090,075 - hisD b2020 0.73 -1.6 2,090,124 + hisD b2020 0.77 -5.2 2,090,124 + hisD b2020 0.77 -4.7 2,090,132 - hisD b2020 0.77 -4.3 2,090,300 - -4.9 2,090,337 - -2.9 2,090,419 + +0.5 2,090,721 - hisC b2021 0.28 -1.9 2,090,808 - hisC b2021 0.36 -3.7 2,090,932 + hisC b2021 0.48 -4.8 2,090,932 + hisC b2021 0.48 -3.8 2,090,938 + hisC b2021 0.48 -5.3 2,091,102 + hisC b2021 0.63 -1.8 2,091,202 - hisC b2021 0.73 -1.9 2,091,202 - hisC b2021 0.73 -3.0 2,091,274 + hisC b2021 0.80 -4.8 2,091,351 + hisC b2021 0.87 -6.8
Or see this region's nucleotide sequence