Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhcG and yhcH are separated by 59 nucleotides yhcH and yhcI overlap by 4 nucleotides yhcI and nanE overlap by 4 nucleotides nanE and nanT are separated by 47 nucleotides
b3220: yhcG - hypothetical protein (NCBI), at 3,365,849 to 3,366,976
yhcG
b3221: yhcH - hypothetical protein (NCBI), at 3,367,036 to 3,367,500
yhcH
b3222: yhcI - putative NAGC-like transcriptional regulator (VIMSS), at 3,367,497 to 3,368,372
yhcI
b3223: nanE - predicted N-acetylmannosamine-6-P epimerase (NCBI), at 3,368,369 to 3,369,058
nanE
b3224: nanT - sialic acid transporter (VIMSS), at 3,369,106 to 3,370,596
nanT
Position (kb)
3367
3368
3369 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3366.683 kb on - strand, within yhcG at 3366.683 kb on - strand, within yhcG at 3366.684 kb on + strand, within yhcG at 3366.684 kb on + strand, within yhcG at 3366.692 kb on - strand, within yhcG at 3366.720 kb on + strand, within yhcG at 3366.760 kb on + strand, within yhcG at 3366.760 kb on + strand, within yhcG at 3366.808 kb on + strand, within yhcG at 3366.856 kb on - strand, within yhcG at 3366.857 kb on + strand, within yhcG at 3366.906 kb on + strand at 3366.924 kb on - strand at 3366.955 kb on - strand at 3366.960 kb on + strand at 3366.960 kb on + strand at 3366.960 kb on + strand at 3366.967 kb on - strand at 3366.968 kb on - strand at 3367.219 kb on - strand, within yhcH at 3367.221 kb on + strand, within yhcH at 3367.238 kb on + strand, within yhcH at 3367.246 kb on - strand, within yhcH at 3367.260 kb on - strand, within yhcH at 3367.273 kb on - strand, within yhcH at 3367.273 kb on - strand, within yhcH at 3367.348 kb on - strand, within yhcH at 3367.386 kb on - strand, within yhcH at 3367.386 kb on - strand, within yhcH at 3367.444 kb on - strand, within yhcH at 3367.452 kb on - strand, within yhcH at 3367.454 kb on + strand at 3367.480 kb on - strand at 3367.480 kb on - strand at 3367.489 kb on + strand at 3367.523 kb on - strand at 3367.600 kb on + strand, within yhcI at 3367.600 kb on + strand, within yhcI at 3367.666 kb on - strand, within yhcI at 3367.678 kb on + strand, within yhcI at 3367.678 kb on + strand, within yhcI at 3367.691 kb on + strand, within yhcI at 3367.691 kb on + strand, within yhcI at 3367.725 kb on + strand, within yhcI at 3367.728 kb on + strand, within yhcI at 3367.768 kb on - strand, within yhcI at 3367.855 kb on - strand, within yhcI at 3367.855 kb on - strand, within yhcI at 3367.901 kb on + strand, within yhcI at 3368.002 kb on + strand, within yhcI at 3368.179 kb on - strand, within yhcI at 3368.179 kb on - strand, within yhcI at 3368.184 kb on - strand, within yhcI at 3368.285 kb on + strand at 3368.327 kb on - strand at 3368.368 kb on + strand at 3368.585 kb on + strand, within nanE at 3368.593 kb on - strand, within nanE at 3368.696 kb on - strand, within nanE at 3368.699 kb on + strand, within nanE at 3368.699 kb on + strand, within nanE at 3368.703 kb on + strand, within nanE at 3368.768 kb on + strand, within nanE at 3368.780 kb on + strand, within nanE at 3368.791 kb on + strand, within nanE at 3368.791 kb on + strand, within nanE at 3368.887 kb on + strand, within nanE at 3368.989 kb on - strand, within nanE at 3368.989 kb on - strand, within nanE at 3369.084 kb on + strand at 3369.297 kb on + strand, within nanT
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 3,366,683 - yhcG b3220 0.74 -2.1 3,366,683 - yhcG b3220 0.74 -2.1 3,366,684 + yhcG b3220 0.74 -0.4 3,366,684 + yhcG b3220 0.74 +0.8 3,366,692 - yhcG b3220 0.75 +0.6 3,366,720 + yhcG b3220 0.77 -0.2 3,366,760 + yhcG b3220 0.81 -0.0 3,366,760 + yhcG b3220 0.81 +0.3 3,366,808 + yhcG b3220 0.85 -2.9 3,366,856 - yhcG b3220 0.89 -0.2 3,366,857 + yhcG b3220 0.89 +0.7 3,366,906 + -0.0 3,366,924 - -0.3 3,366,955 - +0.5 3,366,960 + +0.4 3,366,960 + -2.4 3,366,960 + -1.0 3,366,967 - +0.4 3,366,968 - -0.5 3,367,219 - yhcH b3221 0.39 +0.8 3,367,221 + yhcH b3221 0.40 -1.7 3,367,238 + yhcH b3221 0.43 -0.7 3,367,246 - yhcH b3221 0.45 +0.3 3,367,260 - yhcH b3221 0.48 -1.0 3,367,273 - yhcH b3221 0.51 -0.4 3,367,273 - yhcH b3221 0.51 +0.6 3,367,348 - yhcH b3221 0.67 +0.4 3,367,386 - yhcH b3221 0.75 -0.0 3,367,386 - yhcH b3221 0.75 -0.7 3,367,444 - yhcH b3221 0.88 -0.1 3,367,452 - yhcH b3221 0.89 +0.2 3,367,454 + -1.1 3,367,480 - +0.5 3,367,480 - +0.8 3,367,489 + -2.0 3,367,523 - +0.4 3,367,600 + yhcI b3222 0.12 -4.2 3,367,600 + yhcI b3222 0.12 -0.1 3,367,666 - yhcI b3222 0.19 -0.4 3,367,678 + yhcI b3222 0.21 -0.7 3,367,678 + yhcI b3222 0.21 +0.5 3,367,691 + yhcI b3222 0.22 -2.3 3,367,691 + yhcI b3222 0.22 -3.0 3,367,725 + yhcI b3222 0.26 -0.3 3,367,728 + yhcI b3222 0.26 -0.5 3,367,768 - yhcI b3222 0.31 -1.7 3,367,855 - yhcI b3222 0.41 -0.5 3,367,855 - yhcI b3222 0.41 -0.3 3,367,901 + yhcI b3222 0.46 -2.6 3,368,002 + yhcI b3222 0.58 -2.7 3,368,179 - yhcI b3222 0.78 +0.4 3,368,179 - yhcI b3222 0.78 +0.5 3,368,184 - yhcI b3222 0.78 -0.8 3,368,285 + +1.2 3,368,327 - -0.6 3,368,368 + +0.3 3,368,585 + nanE b3223 0.31 +0.7 3,368,593 - nanE b3223 0.32 -1.2 3,368,696 - nanE b3223 0.47 +0.6 3,368,699 + nanE b3223 0.48 +0.3 3,368,699 + nanE b3223 0.48 -0.1 3,368,703 + nanE b3223 0.48 +0.9 3,368,768 + nanE b3223 0.58 -0.8 3,368,780 + nanE b3223 0.60 -0.8 3,368,791 + nanE b3223 0.61 +0.4 3,368,791 + nanE b3223 0.61 +0.3 3,368,887 + nanE b3223 0.75 +0.7 3,368,989 - nanE b3223 0.90 +1.0 3,368,989 - nanE b3223 0.90 +0.1 3,369,084 + -0.8 3,369,297 + nanT b3224 0.13 -0.2
Or see this region's nucleotide sequence