Experiment: DMSO control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS15215 and ABIE41_RS15220 are separated by 237 nucleotides ABIE41_RS15220 and ABIE41_RS15225 are separated by 31 nucleotides ABIE41_RS15225 and ABIE41_RS15230 are separated by 159 nucleotides ABIE41_RS15230 and ABIE41_RS15235 overlap by 4 nucleotides ABIE41_RS15235 and ABIE41_RS15240 overlap by 14 nucleotides
ABIE41_RS15215: ABIE41_RS15215 - glycerol kinase GlpK, at 3,113,262 to 3,114,779
_RS15215
ABIE41_RS15220: ABIE41_RS15220 - tRNA-Gln, at 3,115,017 to 3,115,090
_RS15220
ABIE41_RS15225: ABIE41_RS15225 - hypothetical protein, at 3,115,122 to 3,115,349
_RS15225
ABIE41_RS15230: ABIE41_RS15230 - glycosyltransferase, at 3,115,509 to 3,116,357
_RS15230
ABIE41_RS15235: ABIE41_RS15235 - SAM-dependent methyltransferase, at 3,116,354 to 3,116,944
_RS15235
ABIE41_RS15240: ABIE41_RS15240 - PIG-L family deacetylase, at 3,116,931 to 3,117,641
_RS15240
Position (kb)
3115
3116
3117 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3114.618 kb on + strand, within ABIE41_RS15215 at 3114.619 kb on - strand, within ABIE41_RS15215 at 3114.809 kb on - strand at 3114.809 kb on - strand at 3114.869 kb on + strand at 3115.365 kb on - strand at 3115.379 kb on + strand at 3115.379 kb on + strand at 3115.379 kb on + strand at 3115.380 kb on - strand at 3115.380 kb on - strand at 3115.384 kb on - strand at 3115.404 kb on + strand at 3115.404 kb on + strand at 3115.405 kb on - strand at 3115.472 kb on + strand at 3115.472 kb on + strand at 3115.472 kb on + strand at 3115.472 kb on + strand at 3115.472 kb on + strand at 3115.472 kb on + strand at 3115.472 kb on + strand at 3115.473 kb on - strand at 3115.473 kb on - strand at 3115.514 kb on - strand at 3115.514 kb on - strand at 3115.738 kb on + strand, within ABIE41_RS15230 at 3115.738 kb on + strand, within ABIE41_RS15230 at 3115.739 kb on - strand, within ABIE41_RS15230 at 3115.739 kb on - strand, within ABIE41_RS15230 at 3115.744 kb on + strand, within ABIE41_RS15230 at 3115.744 kb on + strand, within ABIE41_RS15230 at 3115.744 kb on + strand, within ABIE41_RS15230 at 3115.744 kb on + strand, within ABIE41_RS15230 at 3115.745 kb on - strand, within ABIE41_RS15230 at 3115.745 kb on - strand, within ABIE41_RS15230 at 3115.745 kb on - strand, within ABIE41_RS15230 at 3115.745 kb on - strand, within ABIE41_RS15230 at 3115.745 kb on - strand, within ABIE41_RS15230 at 3116.197 kb on - strand, within ABIE41_RS15230 at 3116.197 kb on - strand, within ABIE41_RS15230 at 3116.388 kb on + strand at 3116.388 kb on + strand at 3116.479 kb on - strand, within ABIE41_RS15235 at 3116.580 kb on + strand, within ABIE41_RS15235 at 3116.581 kb on - strand, within ABIE41_RS15235 at 3116.581 kb on - strand, within ABIE41_RS15235 at 3116.584 kb on - strand, within ABIE41_RS15235 at 3116.841 kb on + strand, within ABIE41_RS15235 at 3116.841 kb on + strand, within ABIE41_RS15235 at 3116.841 kb on + strand, within ABIE41_RS15235 at 3116.841 kb on + strand, within ABIE41_RS15235 at 3116.842 kb on - strand, within ABIE41_RS15235 at 3116.842 kb on - strand, within ABIE41_RS15235 at 3116.842 kb on - strand, within ABIE41_RS15235 at 3116.842 kb on - strand, within ABIE41_RS15235 at 3116.842 kb on - strand, within ABIE41_RS15235 at 3116.842 kb on - strand, within ABIE41_RS15235 at 3116.856 kb on + strand, within ABIE41_RS15235 at 3116.913 kb on + strand at 3116.913 kb on + strand at 3116.932 kb on + strand at 3116.932 kb on + strand at 3116.933 kb on - strand at 3116.933 kb on - strand at 3116.962 kb on + strand at 3116.963 kb on - strand at 3117.142 kb on + strand, within ABIE41_RS15240 at 3117.142 kb on + strand, within ABIE41_RS15240 at 3117.143 kb on - strand, within ABIE41_RS15240 at 3117.143 kb on - strand, within ABIE41_RS15240 at 3117.143 kb on - strand, within ABIE41_RS15240 at 3117.143 kb on - strand, within ABIE41_RS15240 at 3117.209 kb on + strand, within ABIE41_RS15240 at 3117.210 kb on - strand, within ABIE41_RS15240
Per-strain Table
Position Strand Gene LocusTag Fraction DMSO control remove 3,114,618 + ABIE41_RS15215 0.89 +0.6 3,114,619 - ABIE41_RS15215 0.89 -0.1 3,114,809 - +0.5 3,114,809 - +0.0 3,114,869 + -0.7 3,115,365 - +0.1 3,115,379 + +0.9 3,115,379 + -0.5 3,115,379 + +0.0 3,115,380 - -0.7 3,115,380 - +0.9 3,115,384 - -0.5 3,115,404 + +0.4 3,115,404 + -0.5 3,115,405 - -0.2 3,115,472 + -0.4 3,115,472 + +1.3 3,115,472 + +0.4 3,115,472 + +1.9 3,115,472 + -0.3 3,115,472 + -0.0 3,115,472 + +1.0 3,115,473 - +1.6 3,115,473 - -1.6 3,115,514 - -0.2 3,115,514 - -0.1 3,115,738 + ABIE41_RS15230 0.27 +0.2 3,115,738 + ABIE41_RS15230 0.27 +0.2 3,115,739 - ABIE41_RS15230 0.27 -0.5 3,115,739 - ABIE41_RS15230 0.27 +0.8 3,115,744 + ABIE41_RS15230 0.28 +0.3 3,115,744 + ABIE41_RS15230 0.28 +1.0 3,115,744 + ABIE41_RS15230 0.28 +1.9 3,115,744 + ABIE41_RS15230 0.28 -0.5 3,115,745 - ABIE41_RS15230 0.28 +0.2 3,115,745 - ABIE41_RS15230 0.28 +0.0 3,115,745 - ABIE41_RS15230 0.28 +0.0 3,115,745 - ABIE41_RS15230 0.28 +0.1 3,115,745 - ABIE41_RS15230 0.28 +0.1 3,116,197 - ABIE41_RS15230 0.81 +0.1 3,116,197 - ABIE41_RS15230 0.81 -0.9 3,116,388 + -0.2 3,116,388 + -0.8 3,116,479 - ABIE41_RS15235 0.21 -0.3 3,116,580 + ABIE41_RS15235 0.38 -0.0 3,116,581 - ABIE41_RS15235 0.38 +1.0 3,116,581 - ABIE41_RS15235 0.38 +0.1 3,116,584 - ABIE41_RS15235 0.39 +0.3 3,116,841 + ABIE41_RS15235 0.82 -0.5 3,116,841 + ABIE41_RS15235 0.82 +0.7 3,116,841 + ABIE41_RS15235 0.82 +0.3 3,116,841 + ABIE41_RS15235 0.82 -0.5 3,116,842 - ABIE41_RS15235 0.83 -0.6 3,116,842 - ABIE41_RS15235 0.83 +0.1 3,116,842 - ABIE41_RS15235 0.83 +0.4 3,116,842 - ABIE41_RS15235 0.83 -0.5 3,116,842 - ABIE41_RS15235 0.83 -0.3 3,116,842 - ABIE41_RS15235 0.83 +1.6 3,116,856 + ABIE41_RS15235 0.85 +0.9 3,116,913 + -0.1 3,116,913 + -0.4 3,116,932 + +0.2 3,116,932 + +0.3 3,116,933 - +2.9 3,116,933 - +0.3 3,116,962 + -0.1 3,116,963 - -0.3 3,117,142 + ABIE41_RS15240 0.30 +0.2 3,117,142 + ABIE41_RS15240 0.30 +0.4 3,117,143 - ABIE41_RS15240 0.30 -0.3 3,117,143 - ABIE41_RS15240 0.30 -0.6 3,117,143 - ABIE41_RS15240 0.30 -1.6 3,117,143 - ABIE41_RS15240 0.30 +0.5 3,117,209 + ABIE41_RS15240 0.39 +1.1 3,117,210 - ABIE41_RS15240 0.39 +0.8
Or see this region's nucleotide sequence