Experiment: R2A with 3-octanone 2.65516666666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS13805 and ABIE41_RS13810 are separated by 74 nucleotides ABIE41_RS13810 and ABIE41_RS13815 are separated by 36 nucleotides ABIE41_RS13815 and ABIE41_RS13820 overlap by 4 nucleotides ABIE41_RS13820 and ABIE41_RS13825 are separated by 163 nucleotides
ABIE41_RS13805: ABIE41_RS13805 - circularly permuted type 2 ATP-grasp protein, at 2,831,519 to 2,832,934
_RS13805
ABIE41_RS13810: ABIE41_RS13810 - hypothetical protein, at 2,833,009 to 2,833,635
_RS13810
ABIE41_RS13815: ABIE41_RS13815 - DNA repair protein RadC, at 2,833,672 to 2,834,412
_RS13815
ABIE41_RS13820: ABIE41_RS13820 - type I methionyl aminopeptidase, at 2,834,409 to 2,835,233
_RS13820
ABIE41_RS13825: ABIE41_RS13825 - FkbM family methyltransferase, at 2,835,397 to 2,836,263
_RS13825
Position (kb)
2833
2834
2835 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2832.774 kb on + strand, within ABIE41_RS13805 at 2832.883 kb on - strand at 2832.965 kb on - strand at 2832.965 kb on - strand at 2833.026 kb on + strand at 2833.027 kb on - strand at 2833.027 kb on - strand at 2833.027 kb on - strand at 2833.101 kb on + strand, within ABIE41_RS13810 at 2833.227 kb on + strand, within ABIE41_RS13810 at 2833.228 kb on - strand, within ABIE41_RS13810 at 2833.256 kb on - strand, within ABIE41_RS13810 at 2833.256 kb on - strand, within ABIE41_RS13810 at 2833.259 kb on - strand, within ABIE41_RS13810 at 2833.259 kb on - strand, within ABIE41_RS13810 at 2833.403 kb on - strand, within ABIE41_RS13810 at 2833.404 kb on + strand, within ABIE41_RS13810 at 2833.466 kb on - strand, within ABIE41_RS13810 at 2833.471 kb on + strand, within ABIE41_RS13810 at 2833.507 kb on + strand, within ABIE41_RS13810 at 2833.507 kb on + strand, within ABIE41_RS13810 at 2833.519 kb on + strand, within ABIE41_RS13810 at 2833.519 kb on + strand, within ABIE41_RS13810 at 2833.519 kb on + strand, within ABIE41_RS13810 at 2833.519 kb on + strand, within ABIE41_RS13810 at 2833.519 kb on + strand, within ABIE41_RS13810 at 2833.520 kb on - strand, within ABIE41_RS13810 at 2833.520 kb on - strand, within ABIE41_RS13810 at 2833.535 kb on - strand, within ABIE41_RS13810 at 2833.673 kb on + strand at 2833.673 kb on + strand at 2833.673 kb on + strand at 2833.674 kb on - strand at 2833.899 kb on + strand, within ABIE41_RS13815 at 2833.899 kb on + strand, within ABIE41_RS13815 at 2833.899 kb on + strand, within ABIE41_RS13815 at 2834.013 kb on - strand, within ABIE41_RS13815 at 2834.334 kb on - strand, within ABIE41_RS13815 at 2834.809 kb on + strand, within ABIE41_RS13820 at 2834.809 kb on + strand, within ABIE41_RS13820 at 2834.809 kb on + strand, within ABIE41_RS13820 at 2834.809 kb on + strand, within ABIE41_RS13820 at 2834.809 kb on + strand, within ABIE41_RS13820 at 2834.809 kb on + strand, within ABIE41_RS13820 at 2834.810 kb on - strand, within ABIE41_RS13820 at 2834.810 kb on - strand, within ABIE41_RS13820 at 2834.810 kb on - strand, within ABIE41_RS13820 at 2834.810 kb on - strand, within ABIE41_RS13820 at 2834.810 kb on - strand, within ABIE41_RS13820 at 2834.861 kb on - strand, within ABIE41_RS13820 at 2834.861 kb on - strand, within ABIE41_RS13820 at 2834.861 kb on - strand, within ABIE41_RS13820 at 2834.861 kb on - strand, within ABIE41_RS13820 at 2834.905 kb on + strand, within ABIE41_RS13820 at 2834.905 kb on + strand, within ABIE41_RS13820 at 2834.906 kb on - strand, within ABIE41_RS13820 at 2834.906 kb on - strand, within ABIE41_RS13820 at 2835.056 kb on - strand, within ABIE41_RS13820 at 2835.100 kb on + strand, within ABIE41_RS13820 at 2835.101 kb on - strand, within ABIE41_RS13820 at 2835.101 kb on - strand, within ABIE41_RS13820 at 2835.279 kb on - strand at 2835.382 kb on + strand at 2835.382 kb on + strand at 2835.382 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 2.65516666666667 mM remove 2,832,774 + ABIE41_RS13805 0.89 -0.2 2,832,883 - -0.7 2,832,965 - +0.1 2,832,965 - -0.1 2,833,026 + -2.7 2,833,027 - +0.9 2,833,027 - -1.5 2,833,027 - +0.1 2,833,101 + ABIE41_RS13810 0.15 -0.7 2,833,227 + ABIE41_RS13810 0.35 +0.6 2,833,228 - ABIE41_RS13810 0.35 +0.3 2,833,256 - ABIE41_RS13810 0.39 +0.1 2,833,256 - ABIE41_RS13810 0.39 +0.3 2,833,259 - ABIE41_RS13810 0.40 -0.4 2,833,259 - ABIE41_RS13810 0.40 -0.8 2,833,403 - ABIE41_RS13810 0.63 -0.4 2,833,404 + ABIE41_RS13810 0.63 +0.6 2,833,466 - ABIE41_RS13810 0.73 -1.0 2,833,471 + ABIE41_RS13810 0.74 +0.2 2,833,507 + ABIE41_RS13810 0.79 +0.8 2,833,507 + ABIE41_RS13810 0.79 +0.4 2,833,519 + ABIE41_RS13810 0.81 +0.1 2,833,519 + ABIE41_RS13810 0.81 +1.4 2,833,519 + ABIE41_RS13810 0.81 +0.3 2,833,519 + ABIE41_RS13810 0.81 +0.2 2,833,519 + ABIE41_RS13810 0.81 -0.3 2,833,520 - ABIE41_RS13810 0.81 +0.6 2,833,520 - ABIE41_RS13810 0.81 -0.3 2,833,535 - ABIE41_RS13810 0.84 -1.0 2,833,673 + +0.8 2,833,673 + -1.8 2,833,673 + -0.5 2,833,674 - -2.8 2,833,899 + ABIE41_RS13815 0.31 -0.2 2,833,899 + ABIE41_RS13815 0.31 -0.5 2,833,899 + ABIE41_RS13815 0.31 -0.3 2,834,013 - ABIE41_RS13815 0.46 -0.5 2,834,334 - ABIE41_RS13815 0.89 -0.9 2,834,809 + ABIE41_RS13820 0.48 +0.1 2,834,809 + ABIE41_RS13820 0.48 -0.4 2,834,809 + ABIE41_RS13820 0.48 +0.2 2,834,809 + ABIE41_RS13820 0.48 -1.9 2,834,809 + ABIE41_RS13820 0.48 -1.4 2,834,809 + ABIE41_RS13820 0.48 -0.5 2,834,810 - ABIE41_RS13820 0.49 -0.8 2,834,810 - ABIE41_RS13820 0.49 -0.1 2,834,810 - ABIE41_RS13820 0.49 -1.8 2,834,810 - ABIE41_RS13820 0.49 -0.3 2,834,810 - ABIE41_RS13820 0.49 -0.9 2,834,861 - ABIE41_RS13820 0.55 -0.8 2,834,861 - ABIE41_RS13820 0.55 -0.2 2,834,861 - ABIE41_RS13820 0.55 -1.6 2,834,861 - ABIE41_RS13820 0.55 -1.2 2,834,905 + ABIE41_RS13820 0.60 -1.1 2,834,905 + ABIE41_RS13820 0.60 -0.2 2,834,906 - ABIE41_RS13820 0.60 -1.0 2,834,906 - ABIE41_RS13820 0.60 -1.3 2,835,056 - ABIE41_RS13820 0.78 -0.0 2,835,100 + ABIE41_RS13820 0.84 -0.0 2,835,101 - ABIE41_RS13820 0.84 -0.8 2,835,101 - ABIE41_RS13820 0.84 +0.2 2,835,279 - -0.6 2,835,382 + -1.3 2,835,382 + +0.6 2,835,382 + +2.2
Or see this region's nucleotide sequence