Experiment: R2A with 3-octanone 5.31033333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS12510 and ABIE41_RS12515 overlap by 4 nucleotides ABIE41_RS12515 and ABIE41_RS12520 are separated by 31 nucleotides ABIE41_RS12520 and ABIE41_RS12525 are separated by 171 nucleotides
ABIE41_RS12510: ABIE41_RS12510 - ABC transporter permease, at 2,562,811 to 2,564,043
_RS12510
ABIE41_RS12515: ABIE41_RS12515 - ABC transporter ATP-binding protein, at 2,564,040 to 2,564,801
_RS12515
ABIE41_RS12520: ABIE41_RS12520 - efflux RND transporter periplasmic adaptor subunit, at 2,564,833 to 2,566,485
_RS12520
ABIE41_RS12525: ABIE41_RS12525 - N-acetylmuramoyl-L-alanine amidase, at 2,566,657 to 2,567,469
_RS12525
Position (kb)
2564
2565
2566
2567 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2563.897 kb on + strand, within ABIE41_RS12510 at 2563.897 kb on + strand, within ABIE41_RS12510 at 2563.897 kb on + strand, within ABIE41_RS12510 at 2563.897 kb on + strand, within ABIE41_RS12510 at 2564.467 kb on + strand, within ABIE41_RS12515 at 2564.468 kb on - strand, within ABIE41_RS12515 at 2564.468 kb on - strand, within ABIE41_RS12515 at 2564.468 kb on - strand, within ABIE41_RS12515 at 2564.764 kb on + strand at 2564.764 kb on + strand at 2564.765 kb on - strand at 2564.765 kb on - strand at 2564.966 kb on + strand at 2564.967 kb on - strand at 2565.032 kb on + strand, within ABIE41_RS12520 at 2565.032 kb on + strand, within ABIE41_RS12520 at 2565.032 kb on + strand, within ABIE41_RS12520 at 2565.032 kb on + strand, within ABIE41_RS12520 at 2565.033 kb on - strand, within ABIE41_RS12520 at 2565.033 kb on - strand, within ABIE41_RS12520 at 2565.033 kb on - strand, within ABIE41_RS12520 at 2565.033 kb on - strand, within ABIE41_RS12520 at 2565.527 kb on + strand, within ABIE41_RS12520 at 2565.527 kb on + strand, within ABIE41_RS12520 at 2565.527 kb on + strand, within ABIE41_RS12520 at 2565.527 kb on + strand, within ABIE41_RS12520 at 2565.527 kb on + strand, within ABIE41_RS12520 at 2565.527 kb on + strand, within ABIE41_RS12520 at 2565.527 kb on + strand, within ABIE41_RS12520 at 2565.528 kb on - strand, within ABIE41_RS12520 at 2565.528 kb on - strand, within ABIE41_RS12520 at 2565.528 kb on - strand, within ABIE41_RS12520 at 2565.528 kb on - strand, within ABIE41_RS12520 at 2565.674 kb on + strand, within ABIE41_RS12520 at 2565.674 kb on + strand, within ABIE41_RS12520 at 2565.674 kb on + strand, within ABIE41_RS12520 at 2565.674 kb on + strand, within ABIE41_RS12520 at 2565.674 kb on + strand, within ABIE41_RS12520 at 2565.675 kb on - strand, within ABIE41_RS12520 at 2565.675 kb on - strand, within ABIE41_RS12520 at 2565.675 kb on - strand, within ABIE41_RS12520 at 2565.675 kb on - strand, within ABIE41_RS12520 at 2565.675 kb on - strand, within ABIE41_RS12520 at 2566.231 kb on + strand, within ABIE41_RS12520 at 2566.231 kb on + strand, within ABIE41_RS12520 at 2566.232 kb on - strand, within ABIE41_RS12520 at 2566.287 kb on - strand, within ABIE41_RS12520 at 2566.287 kb on - strand, within ABIE41_RS12520 at 2566.287 kb on - strand, within ABIE41_RS12520 at 2566.287 kb on - strand, within ABIE41_RS12520 at 2566.594 kb on + strand at 2566.595 kb on - strand at 2566.595 kb on - strand at 2566.600 kb on - strand at 2566.796 kb on + strand, within ABIE41_RS12525 at 2567.250 kb on - strand, within ABIE41_RS12525 at 2567.321 kb on + strand, within ABIE41_RS12525 at 2567.321 kb on + strand, within ABIE41_RS12525
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.31033333333333 mM remove 2,563,897 + ABIE41_RS12510 0.88 +0.7 2,563,897 + ABIE41_RS12510 0.88 -0.7 2,563,897 + ABIE41_RS12510 0.88 +1.8 2,563,897 + ABIE41_RS12510 0.88 +0.6 2,564,467 + ABIE41_RS12515 0.56 +0.6 2,564,468 - ABIE41_RS12515 0.56 +0.3 2,564,468 - ABIE41_RS12515 0.56 +0.5 2,564,468 - ABIE41_RS12515 0.56 +1.7 2,564,764 + -1.2 2,564,764 + +0.3 2,564,765 - +0.5 2,564,765 - +0.1 2,564,966 + -0.8 2,564,967 - -0.5 2,565,032 + ABIE41_RS12520 0.12 +0.1 2,565,032 + ABIE41_RS12520 0.12 -0.4 2,565,032 + ABIE41_RS12520 0.12 +0.1 2,565,032 + ABIE41_RS12520 0.12 -0.4 2,565,033 - ABIE41_RS12520 0.12 +1.9 2,565,033 - ABIE41_RS12520 0.12 -1.8 2,565,033 - ABIE41_RS12520 0.12 +0.4 2,565,033 - ABIE41_RS12520 0.12 -0.5 2,565,527 + ABIE41_RS12520 0.42 +1.2 2,565,527 + ABIE41_RS12520 0.42 +0.4 2,565,527 + ABIE41_RS12520 0.42 +0.6 2,565,527 + ABIE41_RS12520 0.42 +0.7 2,565,527 + ABIE41_RS12520 0.42 -0.5 2,565,527 + ABIE41_RS12520 0.42 -0.6 2,565,527 + ABIE41_RS12520 0.42 +0.9 2,565,528 - ABIE41_RS12520 0.42 +0.6 2,565,528 - ABIE41_RS12520 0.42 -0.2 2,565,528 - ABIE41_RS12520 0.42 -1.5 2,565,528 - ABIE41_RS12520 0.42 -0.4 2,565,674 + ABIE41_RS12520 0.51 -1.2 2,565,674 + ABIE41_RS12520 0.51 +0.5 2,565,674 + ABIE41_RS12520 0.51 -0.2 2,565,674 + ABIE41_RS12520 0.51 -2.7 2,565,674 + ABIE41_RS12520 0.51 +3.7 2,565,675 - ABIE41_RS12520 0.51 +1.6 2,565,675 - ABIE41_RS12520 0.51 -0.4 2,565,675 - ABIE41_RS12520 0.51 +0.7 2,565,675 - ABIE41_RS12520 0.51 +0.2 2,565,675 - ABIE41_RS12520 0.51 +2.2 2,566,231 + ABIE41_RS12520 0.85 +0.0 2,566,231 + ABIE41_RS12520 0.85 -0.2 2,566,232 - ABIE41_RS12520 0.85 +0.2 2,566,287 - ABIE41_RS12520 0.88 +0.5 2,566,287 - ABIE41_RS12520 0.88 -0.4 2,566,287 - ABIE41_RS12520 0.88 -0.2 2,566,287 - ABIE41_RS12520 0.88 +0.4 2,566,594 + +2.7 2,566,595 - +0.7 2,566,595 - -0.1 2,566,600 - +1.3 2,566,796 + ABIE41_RS12525 0.17 -2.2 2,567,250 - ABIE41_RS12525 0.73 +2.2 2,567,321 + ABIE41_RS12525 0.82 -1.4 2,567,321 + ABIE41_RS12525 0.82 -2.3
Or see this region's nucleotide sequence