Experiment: R2A with 3-octanone 5.31033333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS22405 and ABIE41_RS22410 overlap by 4 nucleotides ABIE41_RS22410 and ABIE41_RS22415 are separated by 1 nucleotides ABIE41_RS22415 and ABIE41_RS22420 are separated by 32 nucleotides ABIE41_RS22420 and ABIE41_RS22425 are separated by 143 nucleotides
ABIE41_RS22405: ABIE41_RS22405 - DUF1214 domain-containing protein, at 4,614,379 to 4,614,954
_RS22405
ABIE41_RS22410: ABIE41_RS22410 - hypothetical protein, at 4,614,951 to 4,615,589
_RS22410
ABIE41_RS22415: ABIE41_RS22415 - hypothetical protein, at 4,615,591 to 4,616,358
_RS22415
ABIE41_RS22420: ABIE41_RS22420 - hypothetical protein, at 4,616,391 to 4,616,621
_RS22420
ABIE41_RS22425: ABIE41_RS22425 - DUF2336 domain-containing protein, at 4,616,765 to 4,617,898
_RS22425
Position (kb) 4615
4616
4617 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4614.874 kb on + strand, within ABIE41_RS22405 at 4614.874 kb on + strand, within ABIE41_RS22405 at 4614.874 kb on + strand, within ABIE41_RS22405 at 4614.875 kb on - strand, within ABIE41_RS22405 at 4614.880 kb on - strand, within ABIE41_RS22405 at 4615.094 kb on + strand, within ABIE41_RS22410 at 4615.095 kb on - strand, within ABIE41_RS22410 at 4615.149 kb on + strand, within ABIE41_RS22410 at 4615.149 kb on + strand, within ABIE41_RS22410 at 4615.150 kb on - strand, within ABIE41_RS22410 at 4615.150 kb on - strand, within ABIE41_RS22410 at 4615.150 kb on - strand, within ABIE41_RS22410 at 4615.150 kb on - strand, within ABIE41_RS22410 at 4615.260 kb on + strand, within ABIE41_RS22410 at 4615.260 kb on + strand, within ABIE41_RS22410 at 4615.261 kb on - strand, within ABIE41_RS22410 at 4615.261 kb on - strand, within ABIE41_RS22410 at 4615.261 kb on - strand, within ABIE41_RS22410 at 4615.261 kb on - strand, within ABIE41_RS22410 at 4615.353 kb on + strand, within ABIE41_RS22410 at 4615.353 kb on + strand, within ABIE41_RS22410 at 4615.353 kb on + strand, within ABIE41_RS22410 at 4615.353 kb on + strand, within ABIE41_RS22410 at 4615.354 kb on - strand, within ABIE41_RS22410 at 4615.587 kb on + strand at 4615.588 kb on - strand at 4615.588 kb on - strand at 4615.601 kb on + strand at 4615.782 kb on - strand, within ABIE41_RS22415 at 4615.787 kb on + strand, within ABIE41_RS22415 at 4615.787 kb on + strand, within ABIE41_RS22415 at 4615.787 kb on + strand, within ABIE41_RS22415 at 4615.788 kb on - strand, within ABIE41_RS22415 at 4615.788 kb on - strand, within ABIE41_RS22415 at 4615.788 kb on - strand, within ABIE41_RS22415 at 4615.788 kb on - strand, within ABIE41_RS22415 at 4616.009 kb on + strand, within ABIE41_RS22415 at 4616.009 kb on + strand, within ABIE41_RS22415 at 4616.010 kb on - strand, within ABIE41_RS22415 at 4616.203 kb on - strand, within ABIE41_RS22415 at 4616.516 kb on - strand, within ABIE41_RS22420 at 4616.711 kb on + strand at 4616.727 kb on - strand at 4617.171 kb on + strand, within ABIE41_RS22425 at 4617.172 kb on - strand, within ABIE41_RS22425
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.31033333333333 mM remove 4,614,874 + ABIE41_RS22405 0.86 +0.7 4,614,874 + ABIE41_RS22405 0.86 +0.1 4,614,874 + ABIE41_RS22405 0.86 -0.2 4,614,875 - ABIE41_RS22405 0.86 +1.5 4,614,880 - ABIE41_RS22405 0.87 +0.4 4,615,094 + ABIE41_RS22410 0.22 -2.0 4,615,095 - ABIE41_RS22410 0.23 -1.7 4,615,149 + ABIE41_RS22410 0.31 +1.2 4,615,149 + ABIE41_RS22410 0.31 -0.2 4,615,150 - ABIE41_RS22410 0.31 +0.1 4,615,150 - ABIE41_RS22410 0.31 +1.0 4,615,150 - ABIE41_RS22410 0.31 +0.6 4,615,150 - ABIE41_RS22410 0.31 -0.2 4,615,260 + ABIE41_RS22410 0.48 +0.8 4,615,260 + ABIE41_RS22410 0.48 +1.1 4,615,261 - ABIE41_RS22410 0.49 -0.2 4,615,261 - ABIE41_RS22410 0.49 +0.6 4,615,261 - ABIE41_RS22410 0.49 +0.5 4,615,261 - ABIE41_RS22410 0.49 -2.1 4,615,353 + ABIE41_RS22410 0.63 +2.1 4,615,353 + ABIE41_RS22410 0.63 +0.3 4,615,353 + ABIE41_RS22410 0.63 -0.9 4,615,353 + ABIE41_RS22410 0.63 +0.3 4,615,354 - ABIE41_RS22410 0.63 +0.9 4,615,587 + -0.9 4,615,588 - +1.1 4,615,588 - -1.4 4,615,601 + -0.5 4,615,782 - ABIE41_RS22415 0.25 +1.8 4,615,787 + ABIE41_RS22415 0.26 +2.1 4,615,787 + ABIE41_RS22415 0.26 +1.3 4,615,787 + ABIE41_RS22415 0.26 +1.1 4,615,788 - ABIE41_RS22415 0.26 -2.7 4,615,788 - ABIE41_RS22415 0.26 -0.2 4,615,788 - ABIE41_RS22415 0.26 -2.6 4,615,788 - ABIE41_RS22415 0.26 +0.3 4,616,009 + ABIE41_RS22415 0.54 -2.4 4,616,009 + ABIE41_RS22415 0.54 -0.3 4,616,010 - ABIE41_RS22415 0.55 -0.5 4,616,203 - ABIE41_RS22415 0.80 +0.5 4,616,516 - ABIE41_RS22420 0.54 -2.9 4,616,711 + +1.0 4,616,727 - -0.2 4,617,171 + ABIE41_RS22425 0.36 -0.6 4,617,172 - ABIE41_RS22425 0.36 -1.4
Or see this region's nucleotide sequence