Experiment: R2A with 3-octanone 5.31033333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS18740 and ABIE41_RS18745 overlap by 4 nucleotides ABIE41_RS18745 and ABIE41_RS18750 overlap by 8 nucleotides ABIE41_RS18750 and ABIE41_RS18755 overlap by 1 nucleotides ABIE41_RS18755 and ABIE41_RS18760 are separated by 28 nucleotides
ABIE41_RS18740: ABIE41_RS18740 - branched-chain amino acid ABC transporter permease, at 3,858,596 to 3,859,678
_RS18740
ABIE41_RS18745: ABIE41_RS18745 - ABC transporter ATP-binding protein, at 3,859,675 to 3,860,478
_RS18745
ABIE41_RS18750: ABIE41_RS18750 - ABC transporter permease, at 3,860,471 to 3,862,720
_RS18750
ABIE41_RS18755: ABIE41_RS18755 - phosphoenolpyruvate hydrolase family protein, at 3,862,720 to 3,863,595
_RS18755
ABIE41_RS18760: ABIE41_RS18760 - flotillin family protein, at 3,863,624 to 3,866,530
_RS18760
Position (kb)
3860
3861
3862
3863 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3859.623 kb on - strand at 3859.623 kb on - strand at 3859.969 kb on + strand, within ABIE41_RS18745 at 3859.969 kb on + strand, within ABIE41_RS18745 at 3859.969 kb on + strand, within ABIE41_RS18745 at 3859.969 kb on + strand, within ABIE41_RS18745 at 3859.970 kb on - strand, within ABIE41_RS18745 at 3859.970 kb on - strand, within ABIE41_RS18745 at 3860.017 kb on + strand, within ABIE41_RS18745 at 3860.017 kb on + strand, within ABIE41_RS18745 at 3860.125 kb on - strand, within ABIE41_RS18745 at 3860.162 kb on - strand, within ABIE41_RS18745 at 3860.162 kb on - strand, within ABIE41_RS18745 at 3860.308 kb on + strand, within ABIE41_RS18745 at 3860.308 kb on + strand, within ABIE41_RS18745 at 3860.308 kb on + strand, within ABIE41_RS18745 at 3860.309 kb on - strand, within ABIE41_RS18745 at 3860.309 kb on - strand, within ABIE41_RS18745 at 3860.309 kb on - strand, within ABIE41_RS18745 at 3860.309 kb on - strand, within ABIE41_RS18745 at 3860.373 kb on + strand, within ABIE41_RS18745 at 3860.374 kb on - strand, within ABIE41_RS18745 at 3860.374 kb on - strand, within ABIE41_RS18745 at 3860.374 kb on - strand, within ABIE41_RS18745 at 3860.440 kb on + strand at 3860.441 kb on - strand at 3860.441 kb on - strand at 3860.441 kb on - strand at 3860.441 kb on - strand at 3860.543 kb on + strand at 3860.543 kb on + strand at 3860.543 kb on + strand at 3860.543 kb on + strand at 3860.544 kb on - strand at 3860.544 kb on - strand at 3860.544 kb on - strand at 3860.747 kb on + strand, within ABIE41_RS18750 at 3860.748 kb on - strand, within ABIE41_RS18750 at 3860.748 kb on - strand, within ABIE41_RS18750 at 3860.850 kb on - strand, within ABIE41_RS18750 at 3861.080 kb on - strand, within ABIE41_RS18750 at 3861.308 kb on + strand, within ABIE41_RS18750 at 3861.309 kb on - strand, within ABIE41_RS18750 at 3861.309 kb on - strand, within ABIE41_RS18750 at 3861.774 kb on + strand, within ABIE41_RS18750 at 3861.774 kb on + strand, within ABIE41_RS18750 at 3861.775 kb on - strand, within ABIE41_RS18750 at 3861.854 kb on + strand, within ABIE41_RS18750 at 3861.855 kb on - strand, within ABIE41_RS18750 at 3861.855 kb on - strand, within ABIE41_RS18750 at 3861.855 kb on - strand, within ABIE41_RS18750 at 3862.217 kb on - strand, within ABIE41_RS18750 at 3862.286 kb on + strand, within ABIE41_RS18750 at 3862.287 kb on - strand, within ABIE41_RS18750 at 3862.368 kb on + strand, within ABIE41_RS18750 at 3862.368 kb on + strand, within ABIE41_RS18750 at 3862.368 kb on + strand, within ABIE41_RS18750 at 3862.368 kb on + strand, within ABIE41_RS18750 at 3862.368 kb on + strand, within ABIE41_RS18750 at 3862.369 kb on - strand, within ABIE41_RS18750 at 3862.369 kb on - strand, within ABIE41_RS18750 at 3862.369 kb on - strand, within ABIE41_RS18750 at 3862.532 kb on + strand at 3862.532 kb on + strand at 3862.533 kb on - strand at 3862.533 kb on - strand at 3862.748 kb on + strand at 3862.861 kb on + strand, within ABIE41_RS18755 at 3862.861 kb on + strand, within ABIE41_RS18755 at 3862.862 kb on - strand, within ABIE41_RS18755 at 3862.941 kb on + strand, within ABIE41_RS18755 at 3862.942 kb on - strand, within ABIE41_RS18755 at 3863.203 kb on + strand, within ABIE41_RS18755 at 3863.203 kb on + strand, within ABIE41_RS18755 at 3863.203 kb on + strand, within ABIE41_RS18755 at 3863.203 kb on + strand, within ABIE41_RS18755 at 3863.203 kb on + strand, within ABIE41_RS18755 at 3863.203 kb on + strand, within ABIE41_RS18755 at 3863.204 kb on - strand, within ABIE41_RS18755 at 3863.204 kb on - strand, within ABIE41_RS18755 at 3863.204 kb on - strand, within ABIE41_RS18755 at 3863.204 kb on - strand, within ABIE41_RS18755 at 3863.204 kb on - strand, within ABIE41_RS18755 at 3863.204 kb on - strand, within ABIE41_RS18755 at 3863.204 kb on - strand, within ABIE41_RS18755 at 3863.204 kb on - strand, within ABIE41_RS18755 at 3863.204 kb on - strand, within ABIE41_RS18755 at 3863.204 kb on - strand, within ABIE41_RS18755 at 3863.211 kb on - strand, within ABIE41_RS18755 at 3863.467 kb on + strand, within ABIE41_RS18755 at 3863.467 kb on + strand, within ABIE41_RS18755 at 3863.696 kb on + strand at 3863.697 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.31033333333333 mM remove 3,859,623 - +0.6 3,859,623 - -0.4 3,859,969 + ABIE41_RS18745 0.37 -0.3 3,859,969 + ABIE41_RS18745 0.37 -1.0 3,859,969 + ABIE41_RS18745 0.37 -0.9 3,859,969 + ABIE41_RS18745 0.37 -1.2 3,859,970 - ABIE41_RS18745 0.37 -1.4 3,859,970 - ABIE41_RS18745 0.37 -0.1 3,860,017 + ABIE41_RS18745 0.43 +0.0 3,860,017 + ABIE41_RS18745 0.43 +0.3 3,860,125 - ABIE41_RS18745 0.56 -0.3 3,860,162 - ABIE41_RS18745 0.61 -1.0 3,860,162 - ABIE41_RS18745 0.61 +0.4 3,860,308 + ABIE41_RS18745 0.79 -0.7 3,860,308 + ABIE41_RS18745 0.79 -1.6 3,860,308 + ABIE41_RS18745 0.79 +0.5 3,860,309 - ABIE41_RS18745 0.79 +0.9 3,860,309 - ABIE41_RS18745 0.79 -0.6 3,860,309 - ABIE41_RS18745 0.79 +0.2 3,860,309 - ABIE41_RS18745 0.79 +0.8 3,860,373 + ABIE41_RS18745 0.87 +0.0 3,860,374 - ABIE41_RS18745 0.87 +2.2 3,860,374 - ABIE41_RS18745 0.87 +0.8 3,860,374 - ABIE41_RS18745 0.87 +0.4 3,860,440 + +0.2 3,860,441 - +1.5 3,860,441 - +0.4 3,860,441 - -1.5 3,860,441 - -3.0 3,860,543 + +0.1 3,860,543 + -2.4 3,860,543 + +0.2 3,860,543 + -1.8 3,860,544 - -0.3 3,860,544 - -1.8 3,860,544 - -0.6 3,860,747 + ABIE41_RS18750 0.12 -4.2 3,860,748 - ABIE41_RS18750 0.12 -0.3 3,860,748 - ABIE41_RS18750 0.12 +1.0 3,860,850 - ABIE41_RS18750 0.17 -2.3 3,861,080 - ABIE41_RS18750 0.27 -0.1 3,861,308 + ABIE41_RS18750 0.37 -1.1 3,861,309 - ABIE41_RS18750 0.37 +2.5 3,861,309 - ABIE41_RS18750 0.37 -0.4 3,861,774 + ABIE41_RS18750 0.58 -0.8 3,861,774 + ABIE41_RS18750 0.58 +2.3 3,861,775 - ABIE41_RS18750 0.58 +0.5 3,861,854 + ABIE41_RS18750 0.61 -1.1 3,861,855 - ABIE41_RS18750 0.62 +0.7 3,861,855 - ABIE41_RS18750 0.62 -1.2 3,861,855 - ABIE41_RS18750 0.62 +0.3 3,862,217 - ABIE41_RS18750 0.78 -0.6 3,862,286 + ABIE41_RS18750 0.81 -3.9 3,862,287 - ABIE41_RS18750 0.81 -0.6 3,862,368 + ABIE41_RS18750 0.84 +0.4 3,862,368 + ABIE41_RS18750 0.84 +1.0 3,862,368 + ABIE41_RS18750 0.84 +0.1 3,862,368 + ABIE41_RS18750 0.84 -0.0 3,862,368 + ABIE41_RS18750 0.84 +2.1 3,862,369 - ABIE41_RS18750 0.84 +0.1 3,862,369 - ABIE41_RS18750 0.84 -0.1 3,862,369 - ABIE41_RS18750 0.84 +1.0 3,862,532 + -0.5 3,862,532 + +1.5 3,862,533 - -0.3 3,862,533 - -2.2 3,862,748 + -2.5 3,862,861 + ABIE41_RS18755 0.16 -1.9 3,862,861 + ABIE41_RS18755 0.16 -0.2 3,862,862 - ABIE41_RS18755 0.16 +0.9 3,862,941 + ABIE41_RS18755 0.25 -1.3 3,862,942 - ABIE41_RS18755 0.25 -0.0 3,863,203 + ABIE41_RS18755 0.55 -0.4 3,863,203 + ABIE41_RS18755 0.55 -0.1 3,863,203 + ABIE41_RS18755 0.55 -0.1 3,863,203 + ABIE41_RS18755 0.55 -1.0 3,863,203 + ABIE41_RS18755 0.55 -2.1 3,863,203 + ABIE41_RS18755 0.55 +1.3 3,863,204 - ABIE41_RS18755 0.55 -0.3 3,863,204 - ABIE41_RS18755 0.55 -1.7 3,863,204 - ABIE41_RS18755 0.55 +1.8 3,863,204 - ABIE41_RS18755 0.55 +0.2 3,863,204 - ABIE41_RS18755 0.55 -0.3 3,863,204 - ABIE41_RS18755 0.55 +0.5 3,863,204 - ABIE41_RS18755 0.55 -3.2 3,863,204 - ABIE41_RS18755 0.55 -2.4 3,863,204 - ABIE41_RS18755 0.55 -2.7 3,863,204 - ABIE41_RS18755 0.55 -1.6 3,863,211 - ABIE41_RS18755 0.56 +0.7 3,863,467 + ABIE41_RS18755 0.85 -0.7 3,863,467 + ABIE41_RS18755 0.85 -0.4 3,863,696 + -0.5 3,863,697 - +0.4
Or see this region's nucleotide sequence